An Increase in Death Rate of Anopheles stephensi Fed on Rabbits Immunized with Mosquito Antigen12

Alger, Nelda E.; Cabrera, Edelberto J.

doi:10.1093/jee/65.1.165

Cited by 77 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the intervention can work against different species of malaria parasites transmitted by different species of mosquitoes. Second, mosquito midgut-based TBI would have the added benefit of potentially decreasing mosquito survivorship and͞or fecundity (20)(21)(22)(23)(24)(25). Third, antimidgut antibodies also may disrupt mosquito digestion͞ absorption enough to retard normal oocyst development in previously infected mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship

Lal

Patterson

Sacci

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The mosquito midgut plays a central role in the sporogonic development of malaria parasites. We have found that polyclonal sera, produced against mosquito midguts, blocked the passage of Plasmodium falciparum ookinetes across the midgut, leading to a significant reduction of infections in mosquitoes. Anti-midgut mAbs were produced that display broad-spectrum activity, blocking parasite development of both P. falciparum and Plasmodium vivax parasites in five different species of mosquitoes. In addition to their parasite transmission-blocking activity, these mAbs also reduced mosquito survivorship and fecundity. These results reveal that mosquito midgut-based antibodies have the potential to reduce malaria transmission in a synergistic manner by lowering both vector competence, through transmission-blocking effects on parasite development, and vector abundance, by decreasing mosquito survivorship and egg laying capacity. Because the intervention can block transmission of different malaria parasite species in various species of mosquitoes, vaccines against such midgut receptors may block malaria transmission worldwide.O f the several hundred species of Anopheles mosquitoes worldwide, only about 20 are major vectors of human malaria. Several components contribute to this fact, including the propensity of a given species to feed on humans, the average longevity of a mosquito species, and the innate ability of a species to permit development of the parasite (i.e., vector competence). Different intervention strategies attempt to reduce malaria transmission by reducing these various components. Reducing vector competence is the primary objective behind two rapidly evolving areas of research in malaria and other vector-borne diseases, namely the replacement of natural vector populations with populations genetically engineered for refractoriness and transmission-blocking immunity (TBI).Transmission-blocking vaccines in malaria are envisioned to inhibit the developmental stages of the malaria parasites in the mosquito midgut. The first 48 h after ingestion of an infective blood meal is the most critical time period for malaria parasite development within the mosquito. It is during this time that the parasite fertilization takes place and the resulting zygotes transform into motile ookinetes. Ookinetes traverse the midgut and lodge between the basal lamina and midgut epithelia, where they encyst and develop into sporozoite-producing oocysts.Several studies with rodent and human malaria parasites have revealed that the ookinete-to-oocyst transition is the most vulnerable link in sporogonic development of malaria parasites (1-3). Thus, the developing parasite stages in the mosquito midgut provide an ideal target for transmission-blocking vaccines. Efforts in transmission-blocking vaccines in malaria have focused primarily on the use of specific parasite antigens as targets of immune intervention in the mosquito midgut. Antibodies against parasite antigens block fertilization and͞or prevent the movement of ookinetes across the...

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship

Lal

Patterson

Sacci

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…To our knowledge, this is the first example of a single antigen that can be targeted by IgG in a mosquito blood meal to consistently and significantly reduce mosquito survivorship. Previous vaccine development strategies targeting mosquitoes have immunized animals with whole mosquito tissues, which could never be employed as a vaccine antigen (Alger and Cabrera, 1972;Almeida and Billingsley, 1998), or against a single midgut antigen. These resulted in small and highly variable reductions in survivorship and fecundity and differed between mosquito species, immunized hosts and trials (Dubin et al, 1948;Alger and Cabrera, 1972;Hatfield, 1988a,b;Ramasamy et al, 1988;Vaughan and Azad, 1988;Srikrishnaraj et al, 1993;Noden et al, 1995;Billingsley, 1998, 2002;Lal et al, 2001;Foy et al, 2003;Billingsley et al, 2008;da Costa et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Mosquitocidal properties of IgG targeting the glutamate-gated chloride channel in three mosquito disease vectors (Diptera: Culicidae)

Meyers

Gray

Foy

2015

Journal of Experimental Biology

View full text Add to dashboard Cite

The glutamate-gated chloride channel (GluCl) is a highly sensitive insecticide target of the avermectin class of insecticides. As an alternative to using chemical insecticides to kill mosquitoes, we tested the effects of purified immunoglobulin G (IgG) targeting the extracellular domain of GluCl from Anopheles gambiae (AgGluCl) on the survivorship of three key mosquito disease vectors: Anopheles gambiae s.s., Aedes aegypti and Culex tarsalis. When administered through a single blood meal, anti-AgGluCl IgG reduced the survivorship of A. gambiae in a dose-dependent manner (LC 50 : 2.82 mg ml −1 , range 2.68-2.96 mg ml −1

show abstract

“…All animals used in these mosquitocidal immunity studies were immunized with a mix of crude or partially purified mosquito antigens. The methods for isolating antigen has varied from using whole ground mosquitoes (1,49) to immunizing with midgut antigens that only bind wheat germ agglutinin (41); also, at least five different mosquito species have been studied. Likewise, the vaccinated host animals ranged from rabbits to guinea pigs to mice, and most studies reported that only one or a few of the animals in the experiment generated an immune response that affected mosquito physiology.…”

Section: Discussionmentioning

confidence: 99%

Induction of Mosquitocidal Activity in Mice Immunized withAnopheles gambiaeMidgut cDNA

Foy¹,

Magalhães²,

Injera³

et al. 2003

Infect Immun

View full text Add to dashboard Cite

Vaccines that induce mosquito-killing (mosquitocidal) activity could substantially reduce the transmission of certain mosquito-borne diseases, especially vaccines against African malaria vectors, such as the mosquito Anopheles gambiae. To generate and characterize antimosquito immunity we immunized groups of mice with two individual A. gambiae midgut cDNAs, Ag-Aper1 (a secreted peritrophic matrix protein) and AgMuc1 (a midgut-bound mucin), and an A. gambiae midgut cDNA library from blood-fed mosquitoes. We observed significantly increased mortality among mosquitoes that fed on either the AgMuc1-or the cDNA library-immunized mice compared to that of controls, but no differences were observed among those fed on Ag-Aper1-immunized mice. Analysis of the humoral and cellular immune responses from mice showed that the induced mosquitocidal effect was associated with immune profiles characterized by elevated tumor necrosis factor alpha and gamma interferon cytokine levels and very low antibody titers. Furthermore, an additional immunization of cDNA library-immunized mice with midgut protein shifted immunity toward a Th2-type immune response, characterized by elevated antibody titers and high interleukin-5 and interleukin-10 cytokine levels; importantly, mosquitoes feeding on these mice exhibited no undo mortality. Finally, when immune sera was ingested by mosquitoes through a membrane feeder, no effect on mosquito mortality was observed, indicating that serum factors alone were not responsible for the mosquitocidal effect. Our results demonstrate that mosquitocidal immunity in mice can be consistently generated by midgut cDNA immunization and suggest this cDNA-induced mosquitocidal immunity is cell mediated.

show abstract

An Increase in Death Rate of Anopheles stephensi Fed on Rabbits Immunized with Mosquito Antigen12

Cited by 77 publications

References 0 publications

Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship

Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship

Mosquitocidal properties of IgG targeting the glutamate-gated chloride channel in three mosquito disease vectors (Diptera: Culicidae)

Induction of Mosquitocidal Activity in Mice Immunized withAnopheles gambiaeMidgut cDNA

Contact Info

Product

Resources

About