An in vivo swine study for xeno-grafts of calcium sulfate-based bone grafts with human dental pulp stem cells (hDPSCs)

Kuo, Ting‐Shen; Lee, Sheng Yang; Wu, Hong Da; Poma, Malosi; Wu, Yu Wei; Yang, Jen Chang

doi:10.1016/j.msec.2015.01.092

Cited by 39 publications

(49 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All but four studies in large‐animal models reported the use of autologous cells; two studies reported either the use of allogeneic adult (De Kok et al ., ) or puppy‐derived cells (Yamada et al ., ) in dogs and two studies reported the use of human dental‐derived cells [dental pulp stem cells (DPSCs) or stem cells from human exfoliated deciduous teeth (SHED)] in dogs (Behnia et al ., ) or minipigs (Kuo et al ., ). Among the small‐animal models, nine studies reported the use of allogeneic cells, including DPSCs (Liu et al ., ), periosteal‐ (Sun et al ., ) or periodontal‐ligament‐derived stem cells (PDLSCs) (Su et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…In one study, an ‘immunomodulatory’ effect of amnion‐derived cells was observed when implanted with β‐TCP scaffolds in immunocompetent rats, via suppression of the physiological host response and milder macrophage infiltration, compared with cell‐free scaffolds (Jiawen et al ., ). Interestingly, two studies reported implantation of SHED or DPSC in large animals – dogs (Behnia et al ., ) and minipigs (Kuo et al ., ) – without adverse reactions. Similar results were reported in other studies of alveolar CSD (not included in the present review because they reported only qualitative outcomes; see the Supplementary material online), following implantation of human‐derived cells in minipigs (placenta‐MSCs; Lee et al ., ), rabbits (adipose‐MSCs; see the Supplementary material online, Linero and Chaparro, 2014), rats (adipose‐MSCs; see the Supplementary material online, Streckbein et al ., 2013; and gingiva‐MSC; see the Supplementary material online, Wang et al ., 2011), and mice (maxillofacial‐BMSCs; see the Supplementary material online, Steinhardt et al ., 2008).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Shanbhag

Pandis

Mustafa

et al. 2016

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Regeneration of large, 'critical-size' bone defects remains a clinical challenge. Bone tissue engineering (BTE) is emerging as a promising alternative to autogenous, allogeneic and biomaterial-based bone grafting. The objective of this systematic review was to answer the focused question: in animal models, do cell-based BTE strategies enhance regeneration in alveolar bone critical-size defects (CSDs), compared with grafting with only biomaterial scaffolds or autogenous bone? Following PRISMA guidelines, electronic databases were searched for controlled animal studies reporting maxillary or mandibular CSD and implantation of mesenchymal stem cells (MSCs) or osteoblasts (OBs) seeded on biomaterial scaffolds. A random effects meta-analysis was performed for the outcome histomorphometric new bone formation (%NBF). Thirty-six studies were included that reported on large- (monkeys, dogs, sheep, minipigs) and small-animal (rabbits, rats) models. On average, studies presented with an unclear-to-high risk of bias and short observation times. In most studies, MSCs or OBs were used in combination with alloplastic mineral-phase scaffolds. In five studies, cells were modified by ex vivo gene transfer of bone morphogenetic proteins (BMPs). The meta-analysis indicated statistically significant benefits in favour of: (1) cell-loaded vs. cell-free scaffolds [weighted mean difference (WMD) 15.59-49.15% and 8.60-13.85% NBF in large- and small-animal models, respectively]; and (2) BMP-gene-modified vs. unmodified cells (WMD 10.06-20.83% NBF in small-animal models). Results of cell-loaded scaffolds vs. autogenous bone were inconclusive. Overall, heterogeneity in the meta-analysis was high (I > 90%). In summary, alveolar bone regeneration is enhanced by addition of osteogenic cells to biomaterial scaffolds. The direction and estimates of treatment effect are useful to predict therapeutic efficacy and guide future clinical trials of BTE. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Shanbhag

Pandis

Mustafa

et al. 2016

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…Calcium sulfate, tricalcium phosphate, and hydroxyapatite are also well-known commercial synthetic ceramic bone grafts. They exhibit resorption periods of 1-2, 6-12 and 12-36 months respectively (30,31). We hypothesized that the resorption period of CaSO 4 would parallel the healing period of a calvarial defect in mice.…”

Section: A Composite Gelatin/caso 4 Scaffold Increases the Expressionmentioning

confidence: 99%

Mesenchymal Stem Cells Within Gelatin/CaSO₄ Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration

Aquino-Martínez

Rodríguez-Carballo

Gámez

et al. 2016

Tissue Engineering Part A

View full text Add to dashboard Cite

The delivery of osteogenic factors is a proven therapeutic strategy to promote bone regeneration. Bone morphogenetic proteins (BMPs) constitute a family of cytokines with well-known osteogenic and bone regenerative abilities. However, clinical uses of BMPs require high doses that have been associated with complications such as osteolysis, ectopic bone formation, or hematoma formation. In the present work, we sought to improve bone tissue engineering through an approach that combines the use of bone marrow-derived mesenchymal stem cells (BMMSCs), composite scaffolds, and osteoinductive agents. We employed a composite gelatin/CaSO4 scaffold that allows for an early expansion of seeded BMMSCs, which is followed by an increased level of osteogenic differentiation after 10 days in culture. Furthermore, this scaffold enhanced bone formation by BMMSCs in a mouse model of critical-sized calvarial defect. More importantly, our results demonstrate that ex vivo pretreatment of BMMSCs with low amounts of BMP-2 (2 nM) and Wnt3a (50 ng/mL) for 24 h cooperatively increases the expression of osteogenic markers in vitro and bone regeneration in the critical-sized calvarial defect mouse model. These data provide a strong rationale for the development of an ex vivo cooperative use of BMP-2 and Wnt3a. Osteogenic factor cooperation might be applied to reduce the required amount of growth factors while obtaining higher therapeutic effects.

show abstract

“…To study the local bone formation using hDPSCs and SHED, cranial defects were created in rats on 11 manuscri-pts, 25 , 35 , 40 , 41 , 44 , 48 , 49 , 51 , 55 , 56 in mice on 4 manuscripts, 17 , 42 , 59 , 72 and in only one manuscript they were made on rabbits. 61 Furthermore, 10 studies created maxillary and mandibular bone defects 29 , 45 , 46 , 52 , 53 , 58 , 60 , 62 , 65 , 69 to assess the potential of hDPSCs for bone tissue engineering. In 3 out of these 10 studies, the researchers used the alveolar bone to test the tissue engineering strategies after extraction of third molars of maxillary 60 and mandibular 29 , 46 regions in humans.…”

Section: The Experimental Designsmentioning

confidence: 99%

“… 69 Six studies were conducted in different animal models. Among these studies, one used rats and created the bone defect in the anterior part of the maxilla, 58 one used rat and created the defect in the left side of mandible, 62 one used rabbits and created the defect between the first premolar and the mental foramen, 45 one used mini pigs to create the defects in the mesial region of the maxilla and mandibular first molars, 52 one used mini pigs and created the defect in front of the mandibular angle, 53 and one used dogs and created the defect on each side of the inferior mandibular border. 65 Furthermore, one study evaluated the potential of hDPSCs for bone tissue engineering in rabbits tibia submitted to distraction osteogenesis 68 and one study investigated the osteogenic potential of hDPSCs in an ovine model of induced osteonecrosis of femoral head.…”

Section: The Experimental Designsmentioning

confidence: 99%

The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

et al. 2018

View full text Add to dashboard Cite

Dental pulp represents a promising and easily accessible source of mesenchymal stem cells for clinical applications. Many studies have investigated the use of human dental pulp stem cells and stem cells isolated from the dental pulp of human exfoliated deciduous teeth for bone tissue engineering in vivo. However, the type of scaffold used to support the proliferation and differentiation of dental stem cells, the animal model, the type of bone defect created, and the methods for evaluation of results were extremely heterogeneous among these studies conducted. With this issue in mind, the main objective of this study is to present and summarize, through a systematic review of the literature, in vivo studies in which the efficacy of human dental pulp stem cells and stem cells from human exfoliated deciduous teeth (SHED) for bone regeneration was evaluated. The article search was conducted in PubMed/MEDLINE and Web of Science databases. Original research articles assessing potential of human dental pulp stem cells and SHED for in vivo bone tissue engineering, published from 1984 to November 2017, were selected and evaluated in this review according to the following eligibility criteria: published in English, assessing dental stem cells of human origin and evaluating in vivo bone tissue formation in animal models or in humans. From the initial 1576 potentially relevant articles identified, 128 were excluded due to the fact that they were duplicates and 1392 were considered ineligible as they did not meet the inclusion criteria. As a result, 56 articles remained and were fully analyzed in this systematic review. The results obtained in this systematic review open new avenues to perform bone tissue engineering for patients with bone defects and emphasize the importance of using human dental pulp stem cells and SHED to repair actual bone defects in an appropriate animal model.

show abstract

An in vivo swine study for xeno-grafts of calcium sulfate-based bone grafts with human dental pulp stem cells (hDPSCs)

Cited by 39 publications

References 31 publications

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Mesenchymal Stem Cells Within Gelatin/CaSO₄ Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration

The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

Contact Info

Product

Resources

About

An in vivo swine study for xeno-grafts of calcium sulfate-based bone grafts with human dental pulp stem cells (hDPSCs)

Cited by 39 publications

References 31 publications

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration

The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

Contact Info

Product

Resources

About

Mesenchymal Stem Cells Within Gelatin/CaSO₄ Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration