An In Vivo Study of Lactobacillus rhamnosus (PTCC 1637) as a New Therapeutic Candidate in Esophageal Cancer

Abstract

Statement of Novelty. Esophageal cancer is one of the most common types of cancer globally. Nowadays, Lactobacilli with probiotic potency is a preventing factor in cancer and many diseases. The anti-tumor properties of these bacteria have been indicated in various studies. Objective. This study is aimed at investigating the effect of probiotic Lactobacillus rhamnosus on esophageal cancer in vivo and in vitro. Methods and Results. In this study, the cytotoxicity effects of L. rhamnosus supernatant and whole-cell culture on a cancer cell line (Kyse30) compared to 5fu were evaluated by the MTT assay. The real-time PCR method was used to analyse the L. rhamnosus supernatant effect on the expression of Wnt signaling pathway genes. An in vivo investigation in nude mice was done to assess the anti-tumor activity of L. rhamnosus supernatant and whole-cell culture. Both supernatant and whole-cell culture of L. rhamnosus reduced cell survival (Kyse30) P < 0.001 . The supernatant of this bacterium significantly reduced the expression of Wnt signaling pathway genes. Administration of supernatant and whole-cell culture of L. rhamnosus expressively reduced tumor growth compared to the control group. The effects of this bacterium on tumor necrosis were quite evident, pathologically P < 0.01 . Conclusion. This study is the first report that assessed the potential impact of L. rhamnosus, especially its supernatant on esophageal cancer and Wnt signaling pathway genes. Therefore, this bacterium can be a harmless candidate for esophageal cancer therapy.

Anticancer Activity of Postbiotic Mediators Derived from Lactobacillus Rhamnosus GG and Lactobacillus Reuteri on Acute Lymphoblastic Leukemia Cells

“…LGG and LR PMs. In contrast to our study, when cells were treated with LR, the increased expression of BAX and casp3 genes in HT29 cells and a decrease in Wnt signaling pathway gene expression has been observed, which impacts cell proliferation and differentiation in Kyse30 cells [24,25]. Furthermore, a study…”

“…The observed reduction in Jurkat cell viability after treatment with LGG and LR PMs is in line with previous research reporting the anticancer potential of probiotics and their derived products, specifically in leukemia [21][22][23]. Our results are consistent with other studies reporting the anticancer effects of probiotic bacteria and their metabolites on various cancer cell lines, including colon, breast, and esophageal cancer [22,24,25]. It should also be noted that the cytotoxic impacts of Lactobacillus strains on cancerous cells are not limited to their live forms.…”

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

“…It has been suggested that the communication between probiotic intestinal bacteria and their mammalian hosts involves immune control by LAB MVs 27 . The significant effects of whole-cell components and supernatants of probiotic lactobacilli in preventing, inhibiting, and treating various cancers, such as lung, colon, breast, colorectal, stomach, and others, are also taken into consideration 31 , 32 . However, to the best of our current knowledge, there have been no studies on the anticancer properties of MVs isolated from probiotic Lentilactobacillus buchneri.…”

Esophageal cancer and bacterial part of gut microbiota – A multidisciplinary point of view

“…Another probiotic Lactobacillus rhamnosus (PTCC 1637) may also be promising in case of esophageal cancer, what has been confirmed in Hashemi-Khah et al. study ( Hashemi-Khah et al., 2022 ). Despite the fact that overall Lactobacillus spp.…”