An in Vivo Quantifiable Model of Cochlear Neuronal Degeneration Induced by Central Process Injury

Sekiya, Takao; Hatayama, Toru; Shimamura, Norihito; Suzuki, Shigeharu

doi:10.1006/exnr.1999.7280

Cited by 34 publications

(58 citation statements)

References 25 publications

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“…To access the nerve a suboccipital craniotomy is performed followed by compression of the nerve and the labyrinthine artery between the brainstem and the temporal bone at the internal auditory meatus. The results have been promising but include rather traumatizing surgery (Sekiya et al, 2000). In vitro rat cochlear studies also report that sodium salicylate can selectively induce auditory neuronal degeneration (Zheng and Gao, 1996).…”

Section: Discussionmentioning

confidence: 96%

β-Bungarotoxin application to the round window: An in vivo deafferentation model of the inner ear

Palmgren

Jin

et al. 2010

Hearing Research

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Section: Discussionmentioning

confidence: 96%

β-Bungarotoxin application to the round window: An in vivo deafferentation model of the inner ear

Palmgren

Jin

et al. 2010

Hearing Research

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“…At the internal auditory meatus of rats, the facial nerve is situated most rostrally and the cochlear nerve is situated most caudally, and the superior and inferior vestibular nerves are situated between the facial and cochlear nerves (15). The internal auditory arteries (IAA) are consistently located in the lower half of the space between the facial nerve and the vestibulocochlear nerve (15).…”

Section: Animal Preparation and Cochlear Ner6e Compression Proceduresmentioning

confidence: 99%

“…In our experimental model, cochlear neuronal degeneration was induced by compression applied on the cerebellopontine (CP) angle portion of the cochlear nerve, and the cochlear nerve was injured by a compression speed-dependent method that has been reported in detail elsewhere (15). Briefly, 30 male Sprague-Dawley rats weighing 550-650 g were anaesthetized with pentobarbital sodium administered intraperitoneally.…”

Section: Animal Preparation and Cochlear Ner6e Compression Proceduresmentioning

confidence: 99%

“…Quantifiable in 6i6o experimental studies in deprivation of the central target have not been performed earlier, probably due to the lack of a dependable experimental model in which the central connection of spiral ganglion cells to the brainstem can be quantifiably and reproducibly interrupted (15). Such injury of the cochlear neurons plays a critical role in various hearing disturbances, as exemplified by complications of surgery for vestibular schwannomas in which the patients' residual hearing is often endangered by intraoperative trauma to the central axon of cochlear neurons (16,17).…”

Section: Introductionmentioning

confidence: 98%

“…the cochlear nucleus (3). We present here the results of our in 6i6o study in which the effectiveness of NGF, BDNF and NT-3 in preventing cochlear neuronal death was investigated in adult rats whose central connection was interrupted, but in whom the peripheral connection was spared (15).…”

Section: Introductionmentioning

confidence: 98%

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Cerebellopontine Angle Cisternal Infusion of NGF, BDNF and NT-3: Effects on Cochlear Neurons Disconnected from Central Target, Cochlear Nucleus: Anin vivoquantitative study

Sekiya

Shimamura²,

Hatayama³

et al. 2000

Acta Oto-Laryngologica

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Cochlear neurons need their synaptic contacts with both their peripheral (organ of Corti) and central (cochlear nucleus) targets for survival. We examined the in vivo effectiveness of the neurotrophins (NGF, BDNF and NT-3) on cochlear neuronal survival using our in vivo model, in which the central connection alone was selectively and quantitatively interrupted. The particular neurotrophins evaluated in the present study did not appear to have cochlear nerve rescue potential. However, the experimental model reported here can serve as a useful tool to investigate cochlear neuronal degeneration from the central side, which may lead to identification of effective mediators in the future.

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Trauma‐specific insults to the cochlear nucleus in the rat

Sekiya

Viberg

Kojima

et al. 2012

J of Neuroscience Research

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The effect of acoustic overstimulation on the neuronal number of the cochlear nucleus (CN) was investigated by using unbiased stereological methods in rats. We found that, after 9 weeks of recovery, neurons in the anteroventral cochlear nucleus (AVCN) degenerated, whereas those in the posteroventral and dorsal cochlear nuclei (PVCN and DCN) were preserved. The noise trauma induced near complete loss of the outer hair cells throughout the cochlea, and the inner hair cells were preserved only in the more apical regions. This pattern of selective loss of AVCN neurons in this study was different from trauma induced by auditory deafferentation by mechanical compression of auditory neurons. In contrast to noise trauma, mechanical compression caused loss of neurons in the PVCN and DCN. After 5 weeks of recovery from mechanical compression, there was no loss of inner or outer hair cells. These findings indicate that auditory deprivation, induced by different experimental manipulations, can have strikingly different consequences for the central auditory system. We hypothesized that AVCN neuronal death was induced by excitotoxic mechanisms via AMPA-type glutamate receptors and that excitatory neuronal circuits developed after acoustic overstimulation protected the PVCN and DCN against neuronal death. The results of the present study demonstrate that hearing loss from different etiologies will cause different patterns of neuronal degeneration in the CN. These findings are important for enhancing the performance of cochlear implants and auditory brainstem implants, because diverse types of hearing loss can selectively affect neuronal degeneration of the CN.

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An in Vivo Quantifiable Model of Cochlear Neuronal Degeneration Induced by Central Process Injury

Cited by 34 publications

References 25 publications

β-Bungarotoxin application to the round window: An in vivo deafferentation model of the inner ear

β-Bungarotoxin application to the round window: An in vivo deafferentation model of the inner ear

Cerebellopontine Angle Cisternal Infusion of NGF, BDNF and NT-3: Effects on Cochlear Neurons Disconnected from Central Target, Cochlear Nucleus: Anin vivoquantitative study

Trauma‐specific insults to the cochlear nucleus in the rat

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