An in vivo analysis of Cr6+ induced biochemical, genotoxicological and transcriptional profiling of genes related to oxidative stress, DNA damage and apoptosis in liver of fish, Channa punctatus (Bloch, 1793)

Awasthi, Yashika; Ratn, Arun; Prasad, R Raghu; Kumar, Manoj; Trivedi, Sunil P.

doi:10.1016/j.aquatox.2018.05.001

Cited by 71 publications

(39 citation statements)

References 63 publications

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“…Results of the present study clearly show that intranasal instillation of PD caused significant depletion of GSH and marked increase of MDA which is mainly attributed to oxidative stress caused by Cr VI. Our results are in consistent with the previous studies which showed that Cr VI induced oxidative stress by increasing MDA as well as decreasing GSH, SOD and CAT anti-oxidant enzyme activities in rats [ 31 , 32 ]. Meanwhile, treatment with L-carnitine significantly protected the cellular constituents via declining MDA and elevating GSH levels, which was previously proven [ 15 ].…”

Section: Discussionsupporting

confidence: 94%

L-carnitine alleviated acute lung injuries induced by potassium dichromate in rats: involvement of Nrf2/HO-1 signaling pathway

Salama

Fayed

Elgohary

2021

Heliyon

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The activation of the Nrf2/HO-1 signaling pathway regulates cellular antioxidant stress and exerts anti-inflammatory and cytoprotective effects against acute lung injury (ALI). The present study aimed to evaluate the therapeutic role of L-carnitine (LC) against potassium dichromate (PD) - induced acute lung injury in adult male albino rats via modulation of Nrf2/HO-1 signaling pathway. For this purpose, forty rats were randomly allocated into 5 groups (8 rats each). The normal group received intranasal (i.n.) saline, while the ALI group received intranasal instillation of PD as a single dose of 2 mg/kg. The 3d – 5th groups received PD then after 24 h administered L-carnitine (25, 50 and 100 mg/kg; orally) for 3 consecutive days. The therapeutic effect of L-carnitine was evaluated by assessment of serum levels of glutathione (GSH) and malondialdehyde (MDA) along with measurement of lung contents of transforming growth factor β1 (TGFβ1), protein kinase B (AKT), Nuclear factor erythroid-2 related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 enzyme (NQO1) and glutathione cysteine ligase modifier subunit (GCLM) expression. Post-treatment with L-carnitine effectively increased the levels of GSH and AKT, elevated Nrf2 and its target genes and decreased the levels of MDA and TGFβ1 in comparison with PD control rats. Additionally, L-carnitine effectively reduced the number of goblet cell, inhibited the mucus formation in bronchioles and interstitial inflammatory infiltrate as well as alleviated the destruction of alveolar walls, and the congestion of blood vessels in lung tissue induced by PD. Our findings showed that L-carnitine may be a promising therapeutic agent against PD-induced acute lung injury.

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Section: Discussionsupporting

confidence: 94%

L-carnitine alleviated acute lung injuries induced by potassium dichromate in rats: involvement of Nrf2/HO-1 signaling pathway

Salama

Fayed

Elgohary

2021

Heliyon

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“…These responses of AChE were similar to the response of EROD activity, but showed higher sensitivity than the EROD activity in both water-column species and benthic species. The physiological response of AChE is generally increased primarily by neurotoxins [66][67][68][69][70][71]. Our measured AChE activity showed significantly greater response in the I z .…”

Section: Detection Of Physiological Activities Based On Acetylcholinementioning

confidence: 52%

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Bae¹,

Atique²,

Yoon³

et al. 2020

Pol. J. Environ. Stud.

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Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T L), and tail extent moment (TEM) in impacted zone (I z) were 2.0-3.6-fold greater than in controls (C z). Nucleus image analysis showed that the nucleus had circular particle forms in the C z as compared with a longitudinal oval form with broken particles from the nucleus in the I z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I z than in the C z. Such DNA damages and greater physiological responses in the I z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.

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“…Healthy specimens (30 ± 5.0 g; 15 ± 1.0 cm) of sh, acquired through shermen from aquatic habitats of Lucknow (Longitude 26° 55′ N, Latitude 80° 59′ E), Uttar Pradesh, India. Fish were transported to the laboratory and treatment of 0.05% KMnO 4 solution for 2-5 min was given, to avoid external infections if any (Awasthi et al, 2018). Fish were acclimatized to laboratory conditions for 15 days in 160 L properly ventilated glass aquaria (100 × 40 × 40 cm 3 ) loaded with 100 L of 15 days aged tap water (APHA, 2012).…”

Section: Methodsmentioning

confidence: 99%

“…The liver and kidney are the primary organs responsible for the regulation of metabolic pathways, maintenance of body equilibrium and detoxi cation (Awasthi et al, 2018;Kumar et al, 2019;Ratn et al, 2018) therefore, mercury can affect these organs severely and impair their functional role by interfering with key physiological and metabolic processes. Assessment of different biomarkers in blood is an imperative and sensitive index, they play a crucial role in toxicological studies, the obtained experimental data help in clinical correlation (Olayode et al, 2020;Perveen et al, 2019).…”

mentioning

confidence: 99%

Mercuric Chloride induced Oxidative Stress, Genotoxicity, Haematological Changes and Histopathological Alterations in Fish Channa Punctatus (Bloch, 1793).

Trivedi

Singh

Trivedi

et al. 2021

Preprint

Self Cite

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The current study was strived to investigate the adverse effects of Mercuric chloride (HgCl2) overload in the fish Channa punctatus. Two sub-lethal test concentrations of HgCl2 viz., 1/20th and 1/10th of 96 h LC50 i.e., 0.03 mg/L and 0.07 mg/L, respectively, were used for exposure. Blood, liver and kidney tissues of the control and exposed specimens were sampled at intervals of 15, 30 and 45 days to assess alterations in oxidative stress, genotoxicity haematological parameters and histopathology. Significant changes in Hb%, RBC count, WBC count, antioxidant enzyme activity viz., super oxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione reductase (GR) were recorded. Micronuclei (MN) induction, nuclear abnormalities (NA) and histopathological alterations were also observed in the exposed fish. Significant (p < 0.05) increase in activities of SOD, CAT, GSH, GR and after 45 d decrease in the level of GSH and GR suggest an undermined anti-oxidative defence system in the fish exposed to HgCl2. Histological examination of liver and kidney showed serious tissue injury and histological alterations. Significant increase in MN and NA frequencies reveal the DNA damage in erythrocytes of fish and haematological changes show the toxicological potential of HgCl2. The observed genotoxicity, biochemical, haematological and histological changes in the present study provide the most extensive insight of HgCl2 stress in Channa punctatus.

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An in vivo analysis of Cr6+ induced biochemical, genotoxicological and transcriptional profiling of genes related to oxidative stress, DNA damage and apoptosis in liver of fish, Channa punctatus (Bloch, 1793)

Cited by 71 publications

References 63 publications

L-carnitine alleviated acute lung injuries induced by potassium dichromate in rats: involvement of Nrf2/HO-1 signaling pathway

L-carnitine alleviated acute lung injuries induced by potassium dichromate in rats: involvement of Nrf2/HO-1 signaling pathway

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Mercuric Chloride induced Oxidative Stress, Genotoxicity, Haematological Changes and Histopathological Alterations in Fish Channa Punctatus (Bloch, 1793).

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