An in vitro study to elucidate the effects of Septilin TM on immune pathways

Abstract

Objective: The use of herbal immunomodulatory preparations to prevent and treat immunological complications is increasing in popularity. Rigorous in vitro, in vivo and clinical trial studies are needed to ensure safety, quality and efficacy for the wellbeing of patients. SeptilinTM, a proprietary herbal medicinal product has been reported to have immunomodulatory effects. This study investigated the in vitro effects of SeptilinTM on biomarkers of specific immune pathways by using whole blood culture assays (WBC).Materials and Methods: Stimulated and unstimulated WBC have been incubated with the product. Enzyme linked immunosorbent assays have been used to screen for IL-6, IL-10, and IFN γ as biomarkers for inflammation, humoral immunity, and cell mediated immunity, respectively.Results: SeptilinTM had no effect on the release of IL-6 production by (lipopolysaccharide) LPS stimulated WBC across all concentrations tested. However, SeptilinTM induced significant higher levels (P<0.001) of IL-6 release in unstimulated WBC across all concentrations between 0µg/ml-258µg/ml. SeptilinTM had no effect on the release of IL-10 release in unstimulated WBC across all concentrations. However the presence of SeptilinTM in phytohaemagglutinin (PHA) stimulated WBC induced significant higher release (P<0.01) of IL-10 release at concentrations between 64.5µg/ml- 258µg/ml when compared to the control. The presence of SeptilinTM in unstimulated WBC had no effect on the release of IFN γ production across all concentrations. The presence of SeptilinTM in PHA stimulated WBC release of IFN γ is inconclusive.Conclusion: This study shows that SeptilinTM has immunomodulatory effects on WBC in vitro.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.238-244

An in vitro study to elucidate the effects of Product Nkabinde on immune response in peripheral blood mononuclear cells of healthy donors

“…Similar findings of increased anti-inflammatory cytokine (IL-10), pro-inflammatory cytokines (IL-1α, IL-1β, TNF-α), and chemokine production were observed in PBMCs treated with the combination of PHA and PN water extracts. This study strengthens evidence that PN can illicit increased production of MIP1-β, IL-1α, and IL-1β after treatment with traditional medicine as previous shown with other traditional medicines ( Barak et al, 2002 ; Denzler et al, 2010 ; Hoosen and Pool, 2018 ). Pro-inflammatory cytokines like TNF-α, IL-1α, IL-1β, and chemokine MIP1-β have been implicated in multiple immunopathological conditions ( Sandeep Varma et al, 2011 ).…”

A Cross-Sectional Analysis of Knowledge, Beliefs and Practices Regarding Traditional Medicine Use among Pharmacy Students of Different Private and Government Universities of Lahore, Pakistan

“…Candidates of the study were also assessed regarding medicinal plants utilization against day to day issues and diseases. The outcome of the current survey showed that majority of the participants were using medicinal plants to treat many ailments and previous studies also supported this investigation as medicinal plants are enriched source of phytochemicals that have potential therapeutic activities to cure diseases 37,38 . However, many of the effects of medicinal plants are not completely understood, yet found effective as therapeutic agents and still there is a need to conduct more research in this area of study 39,40 .…”

Irritation Test and Melanin Whitening Cream Epigallocatechin Gallate Green Tea Leaf with Mexameter and Self-Assessment

“…Table III 25 stated that green tea extract with a concentration of 20% in its flavonoid content had activity as a tyrosinase inhibitor so that it would prevent the formation of melanin due to UV-B rays, polyphenols in green tea could function to protect the skin from UV radiation and inhibit the process melanogenesis so that the increase in the amount of melanin was inhibited and the tannin content in green tea could function as a lightener with an anti-tyrosinase mechanism so that melanin production could be inhibited. The results of this study lacked effectiveness as a lightening agent [26][27][28] . Limitations and shortcomings in this study were the activities of respondents who were exposed to sunlight so that it could inhibit the work of epigallocatechin gallate lightening cream and the inhibition of the malagonesis process was less than optimal.…”