An in vitro study of monosaccharide absorption in echinoid gut

Bamford, David; West, Benjamin A.; Jeal, F

doi:10.1016/0300-9629(72)90438-0

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…This system provides an estimation of the total amount of nutrients and other molecules potentially absorbed by the intestine after enzyme hydrolysis. Nevertheless, a better estimation of nutrient absorption in vitro can be provided by the everted intestine technique (Bamford et al 1972). This uses segments from different parts of the intestine maintained in oxygenated Ringer solution to measure the movement of molecules across the cellular membrane barrier under different conditions.…”

Section: Configurationsmentioning

confidence: 99%

Application of in vitro digestibility methods in aquaculture: constraints and perspectives

Moyano

Rodrigáñez

Díaz

et al. 2014

Reviews in Aquaculture

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In vitro assays simulating the digestion process have been extensively used in the evaluation of the nutritional quality of foods and feeds for humans and terrestrial animals. Such techniques have been comparatively less used in nutrition of aquacultured species, although in recent years the interest in developing alternatives to the conventional in vivo digestibility assays has increased. Using 65 published papers as a reference, this review summarizes the different approaches and configurations used to date in the simulation of the digestion of food by aquatic animals, as well as the main limitations detected in the different methods and the potential advantages derived from their application. Some recommendations are suggested to develop suitable in vitro assays oriented either to the nutritional evaluation of feed ingredients or to get a more detailed knowledge on the interactions among digestive enzymes and feed components.

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Section: Configurationsmentioning

confidence: 99%

Application of in vitro digestibility methods in aquaculture: constraints and perspectives

Moyano

Rodrigáñez

Díaz

et al. 2014

Reviews in Aquaculture

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“…For this reason the present data have been treated in this way. Such corrections factors, which may be accounted for as diffusion components, have been applied in previous studies (Arme & Read, 1968;Southworth & Read, 1972;Manjra & Dusanic, 1972;Bamford, West & Jeal, 1972).…”

Section: Kinetic Studiesmentioning

confidence: 99%

Sugar absorption by foetal and neonatal rat intestine in vitro.

Bamford¹,

Ingham²

1975

The Journal of Physiology

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1. Sugar absorption by foetal and neonatal rat intestine has been examined using an in vitro accumulation technique. 2. The capacity for active sugar absorption is present in the rat intestine by the 17th day of gestation. Considerable variation in uptake occurs during the first month post partum; the greatest uptake place during the first week. 3. The absorption of sugars by the developing gut resembles in several respects that by the mature intestine. However, the adult pattern of functional localization along the intestine is not fully established until after the 3rd week post partum. 4. Kinetic studies indicate that variations with age in the distribution of sugar ‘carriers’ along the intestine, rather than changes in the ‘carriers’ themselves, account for the observed variations in absorption.

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“…The everted intestine system has been used for some time as a model to study nutrient absorption (Bamford et al. 1972) and mainly to modulate drug uptake for the development of new drugs in pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Comparative intestinal absorption of amino acids in rainbow trout (Oncorhynchus mykiss), totoaba (Totoaba macdonaldi) and Pacific bluefin tuna (Thunnus orientalis)

Rosas

Vázquez-Duhalt

Tinoco

et al. 2008

Aquaculture Nutrition

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The kinetics of amino acid absorption in the proximal section of three fish species were studied using an everted intestine technique and a pancreatic digest of casein (Tryptone), as the model amino acid mixture. Fresh intestine of rainbow trout (Oncorhynchus mykiss) was used to set up the experimental system, and results were compared with those obtained using intestines from totoaba (Totoaba macdonaldi) and bluefin tuna fish (Thunnus orientalis). The kinetics of amino acid absorption was sigmoidal for all amino acids and for each species of fish tested. In general, specific absorptions of essential amino acids were higher than those of non‐essential ones. No correlation between the concentration of amino acids in the Tryptone solution and the corresponding absorptions was found. The maximum specific absorption rates of all amino acids for trout were 10 times higher than those determined for totoaba and bluefin tuna. The relative amounts of the different amino acids preferentially absorbed in all three species were different. Results obtained from the everted technique may be applicable to the design of formulated diets for large fish species with commercial value.

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An in vitro study of monosaccharide absorption in echinoid gut

Cited by 11 publications

References 13 publications

Application of in vitro digestibility methods in aquaculture: constraints and perspectives

Application of in vitro digestibility methods in aquaculture: constraints and perspectives

Sugar absorption by foetal and neonatal rat intestine in vitro.

Comparative intestinal absorption of amino acids in rainbow trout (Oncorhynchus mykiss), totoaba (Totoaba macdonaldi) and Pacific bluefin tuna (Thunnus orientalis)

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