An in vitro study of osteoblast vitality influenced by the vitamins C and E

Abstract

Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.g. scurvy). The main interest in this study is that vitamin C and E are known to influence bone formation, e.g. vitamin C plays the key role in the synthesis of collagen, the major component of the extracellular bone matrix.In the present study we evaluate the effect of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) on the proliferation and differentiation of primary bovine osteoblasts in vitro. Starting from standard growth medium we minimized the foetal calf serum to reduce their stimulatory effect on proliferation.An improved growth and an increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin was observed while increasing the ascorbic acid concentration up to 200 μg/ml. Furthermore the effects of α-tocopherol on cell growth and cell differentiation were examined, whereby neither improved growth nor increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin were detected.Further investigations are necessary to target at better supportive effect of vitamins on bone regeneration, and healing.

Ex vivo culture platform for assessment of cartilage repair treatment strategies

“…7C). The equilibrium modulus of the native cartilage tissue was preserved during 56 days of culture, similar to GAG con- 2002; Urban et al, 2012). In contrast to chondrocytes, bone cells require β-glycerophosphate, dexamethasone and serum, providing growth factors (Urban et al, 2012;Langenbach and Handschel, 2013).…”

Effect of vitamin E on human periodontal ligament fibroblasts

“…α -tocopherol is the most active and abundant form of Vitamin E. But it was found that when used individually it does not improve cell growth (14) which is why TRF was the choice of this study. It is worthy to mention that (15) addressed the issue of proliferation using a combination of α-tocopherols and selenium on gingival and periodontal fibroblasts using an in vitro model that mimics periodontal wound-healing process.…”

Coffee Consumption Might Reduce the Risk of Osteopenia/Osteoporosis in Premenopausal Taiwanese Women

“…This could help these individuals in preventing the diseases by modifying their lifestyles like physical activities and nutritional intake. Osteoclastogenesis and osteoblastogenesis are some potential protective mechanisms of Vitamin C's role on bone health [29,30,31,32]. Previous cross-sectional studies have shown positive though inconsistent effects of Vitamin C on osteoporosis [33,34,35,36].…”