Introduction:The intent of the present research was to evaluate the antibacterial efficacy of the two different powers of diode laser and photodynamic therapy PDT by utilizing toluidine blue alone and in combination against Enterococcus faecalis.
Methods:The research was done on seventy five human extracted incisor teeth. Their crowns were removed at cementoenamel junction CEJ then the roots were cleaned and shaped. After autoclaving of the roots, they were injected and infected with Enterococcus faecalis for 72 hours. They were divided randomly to five equal categories according to type of treatment used. Group (I), high power diode laser with wave length 980 nm and output power 2 W for one minute. Group (II), toluidine blue TBO photosensitizer for five minutes. Group (III), low power 635 nm diode laser with output power 220 mw for 30 seconds. Group (IV), toluidine blue was activated by low power diode laser 635 nm and output power 220 mw for 30 seconds. Group (V), toluidine blue was activated by 980 nm diode laser and output power 2 W for one minute.Results: Antibacterial efficacy was assessed before and after treatment. 980 nm diode laser reduced bacteria by 86.4 %, toluidine blue reduced it by 19.19%, 630 nm diode laser reduced it by 13.95%, toluidine blue plus low power 635 nm diode laser reduced it by 88.13 %, and toluidine blue plus high power 980 nm diode laser reduced it by 97.76 %.
Conclusion:The most bacterial reduction was found in group toluidine blue plus high power 980 nm diode laser (group V). The combined technique could be an effective modality for elimination of Enterococcus faecalis from the root canals.