An in vitro Reconstructed Human Skin Equivalent Model to Study the Role of Skin Integration Around Percutaneous Devices Against Bacterial Infection

Bolle, Eleonore C.L.; Verderosa, Anthony D.; Dhouib, Rabeb; Parker, Tony J.; Dargaville, Tim R.; Totsika, Makrina

doi:10.3389/fmicb.2020.00670

Cited by 9 publications

(16 citation statements)

References 49 publications

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“…In addition, Rademacher et al [60] pointed out that blocking AhR in HSEs infected with S. epidermidis resulted in increased growth of S. epidermidis . These models of HSEs are also well suited to study skin infections such as superficial fungal infections ( T. rubrum ) [61, 62] or anaerobic skin infections [63].…”

Section: The Microbiological Impact Of Bacteria Viruses and Fungi On ...mentioning

confidence: 99%

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

Cousin,

Misery,

de Vries

et al. 2023

Dermatology

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Background: This review summarizes uses and new applications for dermatological research of in vitro culture models of human skin explants (HSEs). In the last decade, many innovations have appeared in the literature and an exponential number of studies have been recorded in various fields of application such as process culture engineering, stem cell extractions methodology, or cell-to-cell interaction studies under physiological and pathological conditions, wound-healing, and inflammation. Most studies also concerned pharmacology, cosmetology, and photobiology. However, these topics will not be considered in our review. Summary: A better understanding of the mechanisms driving intercellular relationships, at work in the maintenance of 3D tissue architectures has led to the improvement of cell culture techniques. Many papers have focused on the physiological ways that govern in vitro tissue maintenance of HSEs. The analysis of the necessary mechanical stress, intercellular and cell-matrix interactions, allows the maintenance and prolonged use of HSEs in culture for up to 15 days, regardless of the great variability of study protocols from one laboratory to another and in accordance with the objectives set. Because of their close similarities to fresh skin, HSEs are increasingly used to study skin barrier repair and wound healing physiology. Easy to use in co-culture, this model allows a better understanding of the connections and interactions between the peripheral nervous system, the skin and the immune system. The development of the concept of an integrated neuro-immuno-cutaneous system at work in skin physiology and pathology highlighted by this article represents one of the new technical challenges in the field of in vitro culture of HSE. This review of the literature also reveals the importance of using such models in pathology. As sources of stem cells, HSEs are the basis for the development of new tissue engineering models such as organoids or optical clearing tissues technology. This study identifies the main advances and cross-cutting issues in the use of HSE.

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Section: The Microbiological Impact Of Bacteria Viruses and Fungi On ...mentioning

confidence: 99%

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

Cousin,

Misery,

de Vries

et al. 2023

Dermatology

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“…et al, 2021;Predojević et al, 2022). Similar complex in vitro models have been established to study the host-pathogen interactions in the airway mucosa (Marrazzo et al, 2016;Hasan et al, 2018), intestine (Pearce et al, 2018;García-Díaz et al, 2022) and skin (Bolle et al, 2020), but have not yet been used for evaluation of PnDs. The antimicrobial properties of PnDs have also been evaluated in vivo using several different methods.…”

Section: Investigating the Antimicrobial Effects Of Perinatal Derivat...mentioning

confidence: 99%

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Silini

Ramuta

Pires

et al. 2022

Front. Bioeng. Biotechnol.

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Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration.

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“…This artificial epidermis is regularly comprised of keratinocyte cells, attaining a platform to investigate various mechanisms, such as wound healing and regeneration (Deshayes et al 2018 ). Similar in vitro systems can also host studies for the evaluation of new biocompatible and infection-resistant percutaneous devices (Bolle et al 2020a , b ) or to simply uncover the individual effects of different bacterial strains (Jahanshahi et al 2020 ; Koval et al 2019 ; Popov et al 2014 ; Zinoviev et al 2020 ). In spite of this potential, the majority of in vitro skin models are deprived of an efficient circulatory network, hindering its exposure to biologically relevant immune responses (Kim et al 2019 ; Miyazaki et al 2019 ; Saleh and Bryant 2017 ).…”

Section: Skin Models For the Study Of Driveline Infectionsmentioning

confidence: 99%

“…For instance, the biochemical functionalization of 2-dimensional (2D) silicon and velour substrates with type I collagen is shown to reduce non-specific protein adsorption and enhance fibroblast adhesion (Hussain et al 2016 ), indicating potential benefits from the dermal functionalization of LVAD drivelines. Similarly, fibroblast seeding on top of 3-dimensional (3D) mock drivelines promoted upward epidermal growth, inhibiting bacterial migration in HSE (Bolle et al 2020a , b ). Overall, the strategy of driveline functionalization can exploit previously recorded cell-material relationships to expedite the design of power transfer systems with enhanced biocompatibility and infectious-resistant effects.…”

Section: Alternative Solutions For High-power Transfermentioning

confidence: 99%

Systems of conductive skin for power transfer in clinical applications

Kourouklis

Kaemmel

et al. 2021

Eur Biophys J

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The primary aim of this article is to review the clinical challenges related to the supply of power in implanted left ventricular assist devices (LVADs) by means of transcutaneous drivelines. In effect of that, we present the preventive measures and post-operative protocols that are regularly employed to address the leading problem of driveline infections. Due to the lack of reliable wireless solutions for power transfer in LVADs, the development of new driveline configurations remains at the forefront of different strategies that aim to power LVADs in a less destructive manner. To this end, skin damage and breach formation around transcutaneous LVAD drivelines represent key challenges before improving the current standard of care. For this reason, we assess recent strategies on the surface functionalization of LVAD drivelines, which aim to limit the incidence of driveline infection by directing the responses of the skin tissue. Moreover, we propose a class of power transfer systems that could leverage the ability of skin tissue to effectively heal short diameter wounds. In this direction, we employed a novel method to generate thin conductive wires of controllable surface topography with the potential to minimize skin disruption and eliminate the problem of driveline infections. Our initial results suggest the viability of the small diameter wires for the investigation of new power transfer systems for LVADs. Overall, this review uniquely compiles a diverse number of topics with the aim to instigate new research ventures on the design of power transfer systems for IMDs, and specifically LVADs.

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An in vitro Reconstructed Human Skin Equivalent Model to Study the Role of Skin Integration Around Percutaneous Devices Against Bacterial Infection

Cited by 9 publications

References 49 publications

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

Emergence of New Concepts in Skin Physiopathology through the Use of in vitro Human Skin Explants Models

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Systems of conductive skin for power transfer in clinical applications

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