An in vitro promoting role for hydrogen peroxide in human sperm capacitation

Griveau, J. E.; Renard, Philippe; Lannou, D. Le

doi:10.1111/j.1365-2605.1994.tb01260.x

Cited by 185 publications

(78 citation statements)

References 40 publications

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“…9). As the latter specifically scavenges hydrogen peroxide, these data support the notion that this oxidant is a physiological mediator of sperm function, driving both the tyrosine phosphorylation events associated with sperm capacitation (Bize et al 1991, Griveau et al 1994, Rivlin et al 2004, O'Flaherty et al 2006a and facilitating the acrosome reaction (Hsu et al 1999, O'Flaherty et al 2005. It is interesting to note that overall, phenylalanine is a much more powerful stimulus for the acrosome reaction than progesterone.…”

Section: Discussionsupporting

confidence: 64%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an L-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the case with free radical-mediated phenomena, prolonged exposure of human spermatozoa to phenylalanine resulted in the stimulation of apoptosis as indicated by significant increases in mitochondrial superoxide generation and the activation of intracellular caspases. These results confirm the existence of an LAAO in human spermatozoa with a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis.

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Section: Discussionsupporting

confidence: 64%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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“…Particular attention has been given to the harmful effects of ROS on in vitro spermatozoa and the implications of this in semen preparation in In Vitro Fertilization (IVF) programs. However, some reports have shown that ROS can trigger in vitro physiological sperm functions, such as capacitation and hyperactivated motility (103)(104)(105). These positive effects are strictly related to the equilibrium between ROS and the scavenger systems.…”

Section: Seminal Fluid and Ros Damagementioning

confidence: 99%

Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids PUFA scavenger mechanisms and possible scavenger therapies

Lenzi¹

2000

Front Biosci

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The lipid metabolism in sperm cells is important both as one of the main sources for energy production and for cell structure. The double leaflets of the membrane should be considered not simply as a passive lipid film, but as a very specialized structure. The complete maturation of the sperm cell membrane is attained after testicular lipid biosynthetic processes and after passage through the epididymis. A special composition of membrane phospholipids, rich in polyunsaturated fatty acids (PUFA), and the different composition of sperm and immature germ cell membrane are described and discussed.Testis germ cells as well as epididymal maturing spermatozoa are endowed with enzymatic and nonenzymatic scavenger systems to prevent lipoperoxidative damage. Catalase, superoxide dismutase and GSHdependent oxidoreductases are present in variable amounts in the different developmental stages. Phospholipid hydroperoxide GSH peroxidase (PHGPx) activity and alpha tochopherol of epididymal spermatozoa are considered in detail. Their distribution and roles in caput and cauda epididymal sperm cells are discussed.Seminal plasma also has a highly specialized scavenger system that defends the sperm membrane against lipoperoxidation and the degree of PUFA insaturation acts to achieve the same goal. Systemic predisposition and a number of pathologies can lead to an anti-oxidant/prooxidant disequilibrium. Scavengers, such as GSH, can be used to treat these cases as they can restore the physiological constitution of PUFA in the cell membrane. The results of GSH therapy are presented and discussed. PHOSPHOLIPIDS OF THE SPERM PLASMA MEMBRANE: THEIR PHYSIOLOGY AND ROLE Preliminary remarksSperm membranes play a very active role in sperm fertilization capacity and in sperm-oocyte cross talk, and its biochemical constitution is one of the main field of interest in the study of sperm physiology and pathology. Spermatozoa are polarized cells with structurally and functionally distinct domains. The two leaflets in the membrane of the cap region, overlying the acrosomal vesicle are the domains sensitive to the capacitation stimuli (1). When the various steps of capacitation have induced an increase in the fluidity of the cap region, a fusogenic process, which possesses already the structural premise, starts between this membrane and the outer acrosomal vesicle membrane. The final event consists in the formation of pores that allow a dispersion of the acrosomal enzymes acrosine and hyaluronidase. A

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“…Different oxygen radicals such as superoxide anion, nitric oxide, and hydrogen peroxide have been related to sperm capacitation (Griveau et al 1994, de Lamirande et al 2009) and the addition of ROS yields an increase in PY in a variety of species (Baumber et al 2003, Roy & Atreja 2008, Kota et al 2010, Dona et al 2011. It has recently been shown that ROS generation during capacitation may be necessary for cholesterol efflux from the sperm plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Macías‐García¹,

González‐Fernández²,

Loux³

et al. 2015

Reproduction

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Repeatable methods for IVF have not been established in the horse, reflecting the failure of standard capacitating media to induce changes required for fertilization capacity in equine sperm. One important step in capacitation is membrane cholesterol efflux, which in other species is triggered by cholesterol oxidation and is typically enhanced using albumin as a sterol acceptor. We incubated equine sperm in the presence of calcium, BSA, and bicarbonate, alone or in combination. Bicarbonate induced an increase in reactive oxygen species (ROS) that was abolished by the addition of calcium or BSA. Bicarbonate induced protein tyrosine phosphorylation (PY), even in the presence of calcium or BSA. Incubation at high pH enhanced PY but did not increase ROS production. Notably, no combination of these factors was associated with significant cholesterol efflux, as assessed by fluorescent quantitative cholesterol assay and confirmed by filipin staining. By contrast, sperm treated with methyl-b-cyclodextrin showed a significant reduction in cholesterol levels, but no significant increase in PY or ROS. Presence of BSA increased sperm binding to bovine zonae pellucidae in all three stallions. These results show that presence of serum albumin is not associated with a reduction in membrane cholesterol levels in equine sperm, highlighting the failure of equine sperm to exhibit core capacitation-related changes in a standard capacitating medium. These data indicate an atypical relationship among cholesterol efflux, ROS production, and PY in equine sperm. Our findings may help to elucidate factors affecting failure of equine IVF under standard conditions.

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An in vitro promoting role for hydrogen peroxide in human sperm capacitation

Cited by 185 publications

References 40 publications

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids PUFA scavenger mechanisms and possible scavenger therapies

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

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