An in vitro model for characterizing the post‐migratory cranial neural crest cells of the first branchial arch

Zhao, Hu; Bringas, Pablo; Chai, Yang

doi:10.1002/dvdy.20588

Cited by 33 publications

(33 citation statements)

References 43 publications

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“…The neural tube has been used as the source of migratory neural crest cells in vitro in experiments with other vertebrates as well, including the mouse (e.g., Lo et al, 1997;Bannerman et al, 2000;Epstein et al, 2000) and the axolotl (e.g., Cerny et al, 2005). Other laboratories have obtained migratory neural crest cells from isolated neural folds (Basch et al, 2000) or branchial arches (Zhao et al, 2006); however, there is no universally accepted standard model for studying neural crest cells in vitro, and our having chosen the neural tube explant model makes the present results more comparable with our earlier work.…”

Section: Neural Crest Cell Culturesupporting

confidence: 67%

Microarray analysis of homocysteine‐responsive genes in cardiac neural crest cells in vitro

Rosenquist

Bennett

Brauer

et al. 2007

Developmental Dynamics

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The amino acid homocysteine increases in the serum when there is insufficient folic acid or vitamin B 12 , or with certain mutations in enzymes important in methionine metabolism. Elevated homocysteine is related to increased risk for cardiovascular and other diseases in adults and elevated maternal homocysteine increases the risk for certain congenital defects, especially those that result from abnormal development of the neural crest and neural tube. Experiments with the avian embryo model have shown that elevated homocysteine perturbs neural crest/neural tube migration in vitro and in vivo. Whereas there have been numerous studies of homocysteine-induced changes in gene expression in adult cells, there is no previous report of a homocysteine-responsive transcriptome in the embryonic neural crest. We treated neural crest cells in vitro with exogenous homocysteine in a protocol that induces significant changes in neural crest cell migration. We used microarray analysis and expression profiling to identify 65 transcripts of genes of known function that were altered by homocysteine. The largest set of effected genes (19) included those with a role in cell migration and adhesion. Other major groups were genes involved in metabolism (13); DNA/RNA interaction (11); cell proliferation/apoptosis (10); and transporter/receptor (6). Although the genes identified in this experiment were consistent with prior observations of the effect of homocysteine upon neural crest cell function, none had been identified previously as response to homocysteine in adult cells.

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Section: Neural Crest Cell Culturesupporting

confidence: 67%

Microarray analysis of homocysteine‐responsive genes in cardiac neural crest cells in vitro

Rosenquist

Bennett

Brauer

et al. 2007

Developmental Dynamics

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“…The original method for isolating NC progenitors was to culture neural tubes, wait for NCCs to start migrating and then select for p75 + cells [18]. However, using Wnt1-Cre/R26R transgenic mouse, Zhao et al [20], have demonstrated that some non-crest cells also migrate. Additionally, p75 expression is not restricted to progenitor cells but is also observed in differentiated cells.…”

Section: Discussionmentioning

confidence: 99%

“…Postmigratory cranial NCCs have also been isolated from the first branchial arch of E10.5 mouse embryos [20] [21]. Amazingly, postmigratory cranial NCCs transplanted into a host with a calvaria defect differentiated into osteocytes and contributed to the repair of the defect.…”

Section: Isolation Of Ncscsmentioning

confidence: 99%

Neural crest stem cells: discovery, properties and potential for therapy

2012

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Neural crest (NC) cells are a migratory cell population synonymous with vertebrate evolution. They generate a wide variety of cell and tissue types during embryonic and adult development including cartilage and bone, connective tissue, pigment and endocrine cells as well as neurons and glia amongst many others. Such incredible lineage potential combined with a limited capacity for self-renewal, which persists even into adult life, demonstrates that NC cells bear the key hallmarks of stem and progenitor cells. In this review, we describe the identification, characterization and isolation of NC stem and progenitor cells from different tissues in both embryo and adult organisms. We discuss their specific properties and their potential application in cell-based tissue and disease-specific repair.

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“…Furthermore, by providing critical feedback to the surface ectoderm, CNC can provide species-specific patterning information during craniofacial development, highlighting the importance of tissue-tissue interaction in regulating organogenesis (Schneider and Helms, 2003;Helms et al, 2005). In the mouse model, it has been demonstrated elegantly that CNC cells retain a remarkable degree of plasticity, even after their migration into the branchial arch (Zhao et al, 2006). The second arch CNC cells have a cell-autonomous requirement for the Hoxa2 gene for their intrinsic patterning program (Santagati et al, 2005).…”

Section: Concerted Action Of Growth and Transcription Factors In Detementioning

confidence: 99%

“…The second arch CNC cells have a cell-autonomous requirement for the Hoxa2 gene for their intrinsic patterning program (Santagati et al, 2005). In vitro studies have also begun to address the plasticity of postmigratory CNC cells (Zhao et al, 2006). Taken together, it is clear that the fate of mouse postmigratory CNC cells is determined through the reciprocal signaling between neural crest mesenchyme and the surrounding environment, where timing is an essential component.…”

Section: Concerted Action Of Growth and Transcription Factors In Detementioning

confidence: 99%

Recent advances in craniofacial morphogenesis

Chai

Maxson

2006

Developmental Dynamics

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536

503

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Craniofacial malformations are involved in three fourths of all congenital birth defects in humans, affecting the development of head, face, or neck. Tremendous progress in the study of craniofacial development has been made that places this field at the forefront of biomedical research. A concerted effort among evolutionary and developmental biologists, human geneticists, and tissue engineers has revealed important information on the molecular mechanisms that are crucial for the patterning and formation of craniofacial structures. Here, we highlight recent advances in our understanding of evo-devo as it relates to craniofacial morphogenesis, fate determination of cranial neural crest cells, and specific signaling pathways in regulating tissue-tissue interactions during patterning of craniofacial apparatus and the morphogenesis of tooth, mandible, and palate. Together, these findings will be beneficial for the understanding, treatment, and prevention of human congenital malformations and establish the foundation for craniofacial tissue regeneration. Developmental Dynamics 235:2353-2375, 2006.

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An in vitro model for characterizing the post‐migratory cranial neural crest cells of the first branchial arch

Cited by 33 publications

References 43 publications

Microarray analysis of homocysteine‐responsive genes in cardiac neural crest cells in vitro

Microarray analysis of homocysteine‐responsive genes in cardiac neural crest cells in vitro

Neural crest stem cells: discovery, properties and potential for therapy

Recent advances in craniofacial morphogenesis

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