An in vitro model for adhesion of bacteria to human tooth root surfaces

Switalski, Lech M.; Butcher, Wade G.

doi:10.1016/0003-9969(94)90111-2

Cited by 7 publications

(7 citation statements)

References 24 publications

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“…Apparently, this is due to saturation of specific receptors, as well as to covering and, in a sense, neutralization of components mediating the charge interactions. A role for collagen recognition in bacterial adherence has been documented by other recent studies Switalski et al, 1993;Switalski and Butcher, 1994) in which streptococcal and staphylococcal strains were shown to bind soluble collagen as well as attach to collagenous substrata.…”

Section: Discussionmentioning

confidence: 82%

“…Two strains were selected for further characterization of the interaction: L. casei rhamnosus 103, which bound collagen, henceforth referred to as a positive strain; and L. casei rhamnosus 101, which did not bind collagen, referred to as a negative strain. These strains were previously characterized with respect to attachment to dentin (Switalski and Butcher, 1994), which correlates with their ability to recognize collagen.…”

Section: Resultsmentioning

confidence: 99%

“…# 101 and #103 were kindly provided by Dr. J.J. Zambon (State University of New York, Buffalo). These strains were speciated by means of a commercially available kit (API Rapid CH; Analytab Products, Plainview, NY) and classified as L. casei rhamnosus (Switalski and Butcher, 1994). These and other strains were grown on MRS agar (Difco) in an atmosphere of 80% N2, 10% H2, and 10% CO2 at 37°C, overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Binding of 1251-labeled collagen type I to two strains of L. casei rhamnosus has been characterized in some detail. These strains were previously characterized with respect to their attachment to dentin (Switalski and Butcher, 1994). The process of 1251-collagen binding was mediated via specific as well as charge interactions.…”

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confidence: 99%

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Specific and Charge Interactions Mediate Collagen Recognition by Oral Lactobacilli

et al. 1995

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The mechanisms by which oral lactobacilli, one of the three major genera of cariogenic bacteria, attach to tooth surfaces are unknown. We hypothesize that recognition of collagen, the major component of dentin, may be a mechanism which localizes these bacteria to exposed root surfaces as well as to carious lesions which have penetrated the dentin. We found that the majority of oral Lactobacillus spp. strains recognize and bind collagen type I. Binding of 125I-labeled collagen type I to two strains of L. casei rhamnosus has been characterized in some detail. These strains were previously characterized with respect to their attachment to dentin (Switalski and Butcher, 1994). The process of 125I-collagen binding was mediated via specific as well as charge interactions. The putative adhesin-mediated (specific) interaction involved a limited number of bacterial surface components (2 x 10(3)/cell). Under conditions conducive for non-specific interactions (low ionic strength), the binding was higher by an order of magnitude. Collagen binding strains were found to adhere to collagen-coated surfaces, while strains unable to bind collagen adhered to a much lesser extent. Adherence of bacteria to collagen-coated surfaces could be competitively inhibited with collagen. These interactions may target collagen-binding strains of lactobacilli to dentin collagen in the oral cavity and thus play a role in the pathogenesis of root surface and/or coronal caries. Interference with this collagen-mediated attachment of lactobacilli may provide effective means of caries control, particularly in view of the fact that other oral acidogenic microbiota also interact with collagen.

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Section: Discussionmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Specific and Charge Interactions Mediate Collagen Recognition by Oral Lactobacilli

et al. 1995

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“…S. mutans fimbria preparations of caries-free clinical isolates had more fimbrial components, glucosyltransferase and P1 antigen than caries-active preparations (21). Decreases in the amount of P1 on the cell surface have been related to a decrease in cell hydrophobicity and adhesion to tooth surfaces (7,33), while glucosyltransferase is implicated in promoting the accumulation of streptococci on teeth (25). The current study may provide preliminary evidence that physiological differences between caries-free, caries-active and root caries isolates such as P1, glucosyltransferase or fimbria antigens may interfere with polymorphonuclear leukocyte recognition and/or activation.…”

Section: Discussionmentioning

confidence: 99%

Differential activation of human neutrophils by Streptococcus mutans isolates from root surface lesions and caries‐free and caries‐active subjects

Moore

Gregory

Switalski

et al. 1998

Oral Microbiology and Immunology

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Phagocytosis of bacterial pathogens is an important defense mechanism and may contribute to regulating Streptococcus mutans-induced dental caries, particularly at root surfaces. This study was undertaken to examine and compare differences in polymorphonuclear leukocyte or neutrophil activation by clinical isolates of S. mutans collected from the saliva of caries-free or caries-active individuals with S. mutans isolates from root surface lesions. S. mutans clinical isolates (5 caries-free, 5 caries-active, 5 root caries isolates and a laboratory strain) were incubated with neutrophils in the presence of normal human serum and the luminol dependent chemiluminescence was measured for 1 h at 37 degrees C. Results indicated that the caries active and laboratory strains activated neutrophils equally. The mean integration stimulated by caries-free strains, however, displayed a 25-30% enhanced neutrophil activation over the caries-active and laboratory strains. In contrast, neutrophil activation by root caries strains of S. mutans was 45-50% lower than all other S. mutans strains, possibly suggesting a natural selection for S. mutans strains that can evade neutrophil recognition and subsequent phagocytosis. Stimulation of neutrophils with the cell wall and membrane surface component preparations indicated that extracts from all four groups activated neutrophils significantly. Again, caries-free preparations activated neutrophils significantly more than caries active, laboratory strain and root caries isolates. This selection may become more important on root surfaces due to increased exposure to crevicular fluid and neutrophils. The data provide evidence for the presence or onset of mechanisms or biological alterations in S. mutans developed to circumvent neutrophil recognition and/or phagocytosis, thus increasing S. mutans survival and colonization on tooth surfaces, resulting in an enhanced risk of dental caries, particularly at root surfaces.

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Adsorption of saliva-coated and plain streptococcal cells to the surfaces of hydroxyapatite beads

Tanaka

Ebara

Otsuka

et al. 1996

Archives of Oral Biology

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An in vitro model for adhesion of bacteria to human tooth root surfaces

Cited by 7 publications

References 24 publications

Specific and Charge Interactions Mediate Collagen Recognition by Oral Lactobacilli

Specific and Charge Interactions Mediate Collagen Recognition by Oral Lactobacilli

Differential activation of human neutrophils by Streptococcus mutans isolates from root surface lesions and caries‐free and caries‐active subjects

Adsorption of saliva-coated and plain streptococcal cells to the surfaces of hydroxyapatite beads

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