An in vitro fermentation study on the effects of Dendrobium officinale polysaccharides on human intestinal microbiota from fecal microbiota transplantation donors

Effects of different types of potato resistant starches on intestinal microbiota and short‐chain fatty acids under in vitro fermentation

“…The F/B ratio was reported as a biomarker for obesity (Dalby et al, 2017). In this study, the F/B ratio in each group was reduced relative to the BO group, suggesting that RS1, RS2, RS3a, RS3b, RS4, RSM and inulin could prevent obesity (Fu et al, 2019). RS2 showed a higher F/B reduction rate, whereas RS1, RS3a, RS3b, RS4, RSM and inulin showed similar F/B rates (Fig.…”

In vitro fecal fermentation characteristics of bamboo shoot (Phyllostachys edulis) polysaccharide

“…The total bacterial genomic DNA of the fermentation samples (1 mL) was extracted using the QIAamp Fast DNA Stool Mini kit (QIAGEN, Valencia, CA, United States) according to the manufacturer’s manual. The DNA integrity was confirmed by 1% agarose gel electrophoresis ( Fu et al, 2019 ), and the microorganism DNA concentrations were subsequently verified using a Nanodrop 3300 fluorospectrometer (Thermo Fisher Scientific, Massachusetts, USA). The primers 515F and 806R ( Caporaso et al, 2012 ) were used to amplify the V3–V4 variable region of the bacterial 16S rRNA gene.…”

Ecological Adaptation and Succession of Human Fecal Microbial Communities in an Automated In Vitro Fermentation System

“…Previous in vitro studies employed short-chain fatty acid (SCFA) concentrations as a proxy to determine microbial community stability ( 37 – 39 ). SCFAs are predominantly produced by a limited number of bacteria ( 40 ), and there is compelling evidence from both in vitro and in vivo studies that the SCFA concentrations might not reflect the community structure ( 41 – 43 ).…”