An In vitro evaluation of effect of implant abutment on human gingival epithelial keratinocytes

Bagchi, Paulami; Alfawzan, Ahmed Ali; Magar, Swaroopkumar M; Priya, Ritu; Kochhar, Anuraj Singh; Agrawal, Sneh; Almutairi, Faris Jaser

doi:10.4103/aam.aam_116_20

Cited by 4 publications

(1 citation statement)

References 31 publications

(34 reference statements)

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“…The two cell types differ in shape, behavior, and reaction to external stimuli [114][115][116][117][118][119][120][121][122][123][124][125][126]. Various dental restorations and protheses such as crowns, implant abutments, and tissue bars, are surrounded by both connective tissue and junctional epithelium [115,[127][128][129][130][131][132][133][134][135][136]. The present, successful results warrant further studies on epithelial cells for potential synergistic benefits to establish soft tissue seal around zirconia.…”

Section: Discussionmentioning

confidence: 91%

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Suzumura,

Matsuura,

Komatsu

et al. 2023

Cells

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Soft tissue adhesion and sealing around dental and maxillofacial implants, related prosthetic components, and crowns are a clinical imperative to prevent adverse outcomes of periodontitis and periimplantitis. Zirconia is often used to fabricate implant components and crowns. Here, we hypothesized that UV treatment of zirconia would induce unique behaviors in fibroblasts that favor the establishment of a soft tissue seal. Human oral fibroblasts were cultured on zirconia specimens to confluency before placing a second zirconia specimen (either untreated or treated with one minute of 172 nm vacuum UV (VUV) light) next to the first specimen separated by a gap of 150 µm. After seven days of culture, fibroblasts only transmigrated onto VUV-treated zirconia, forming a 2.36 mm volume zone and 5.30 mm leading edge. Cells migrating on VUV-treated zirconia were enlarged, with robust formation of multidirectional cytoplastic projections, even on day seven. Fibroblasts were also cultured on horizontally placed and 45° and 60° tilted zirconia specimens, with the latter configurations compromising initial attachment and proliferation. However, VUV treatment of zirconia mitigated the negative impact of tilting, with higher tilt angles increasing the difference in cellular behavior between control and VUV-treated specimens. Fibroblast size, perimeter, and diameter on day seven were greater than on day one exclusively on VUV-treated zirconia. VUV treatment reduced surface elemental carbon and induced superhydrophilicity, confirming the removal of the hydrocarbon pellicle. Similar effects of VUV treatment were observed on glazed zirconia specimens with silica surfaces. One-minute VUV photofunctionalization of zirconia and silica therefore promotes human oral fibroblast attachment and proliferation, especially under challenging culture conditions, and induces specimen-to-specimen transmigration and sustainable photofunctionalization for at least seven days.

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Section: Discussionmentioning

confidence: 91%

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Suzumura,

Matsuura,

Komatsu

et al. 2023

Cells

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Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Mu,

Wang,

et al. 2024

Phys. Scr.

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The gingival epithelium plays a crucial role in achieving long-term stability of dental implants, and the hydrogenated TiO2 nanotubes with a superhydrophilic surface exhibit more excellent biological activity than pure titanium implants. However, the effects of the hydrogenated TiO2 nanotubes on human gingival epithelial cells remain unclear. Here, we fabricated hydrogenated TiO2 nanotubes using anodization and hydrogenation to investigate the adhesion of human gingival epithelial cells (HGEs) on structured surfaces in vitro. The topography, roughness, and wettability of three sample types—titanium (Ti), TiO2 nanotubes (TNTs), and hydrogenated TiO2 nanotubes (H2-TNTs)—were characterized. To evaluate cell adhesion, the HGEs were co-cultured with these specimens. This allowed for the examination of both the adhesion morphology and the number of cells adhering to each material's surface. Expression levels of genes and proteins related to cell adhesion were also assessed. H2-TNTs demonstrated nanoscale topography similar to TNTs in terms of diameter and height and maintained a superhydrophilic surface (with a static water contact angle of < 5°). The number of HGEs adhering to H2-TNTs was notably higher. Furthermore, HGEs on H2-TNTs displayed a more stretched morphology in comparison to the other two groups. Notably, the expression levels of adhesion-related genes and proteins in H2-TNTs surpassed those of the other two groups. Hence superhydrophilic H2-TNTs significantly enhance the adhesion ability of HGEs on the material surface.

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Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

et al. 2022

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Objectives: This study aimed to investigate the response of human gingival fibroblasts (HGFB) and human gingival keratinocytes (HGKC) towards different dental implant abutment materials. Methods: Five materials were investigated: (1) titanium (Ti), (2) titanium nitride (TiN), (3) cobalt-chromium (CoCr), (4) zirconia (ZrO2), and (5) modified polyether ether ketone (m-PEEK). Both cell lines were cultured, expanded, and seeded in accordance with the protocol of their supplier. Cell proliferation and cytotoxicity were evaluated at days 1, 3, 5, and 10 using colourimetric viability and cytotoxicity assays. Data were analysed via two-way ANOVA, one-way ANOVA, and Tukey’s post hoc test (p < 0.05 for all tests). Results: There was a statistically significant difference in cell proliferation of HGKC and HGFB cells in contact with different abutment materials at different time points, with no significant interaction between different materials. There was a significant effect on cell proliferation and cytotoxicity with different exposure times (p < 0.0001) for each material. Cell proliferation rates were comparable for both cell lines at the beginning of the study, however, HGFB showed higher proliferation rates for all materials at day 10 with better proliferation activities with ZrO and m-PEEK (40.27%) and (48.38%) respectively. HGKC showed significant interactions (p < 0.0001) in cytotoxicity between different materials. Conclusion: The present in vitro assessment investigated the biocompatibility of different abutment materials with soft tissue cells (HGFB and HGKC). The findings suggest that m-PEEK and TiN are biologically compatible materials with human cells that represent the soft tissue and can be considered as alternative implant abutment materials to Ti and ZrO2, especially when the aesthetic is of concern.

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An In vitro evaluation of effect of implant abutment on human gingival epithelial keratinocytes

Cited by 4 publications

References 31 publications

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

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An In vitro evaluation of effect of implant abutment on human gingival epithelial keratinocytes

Cited by 4 publications

References 31 publications

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Anodic oxidation- and hydrogenation-produced TiO2 nanotubes on titanium and its effect on gingival epithelial cell adhesion ability

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

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Anodic oxidation- and hydrogenation-produced TiO₂ nanotubes on titanium and its effect on gingival epithelial cell adhesion ability