An in vitro evaluation of fibrinogen and gelatin containing cryogels as dermal regeneration scaffolds

Allan, Iain; Tolhurst, Bryony A.; Shevchenko, Rostislav V.; Dainiak, Maria B.; Illsley, Matthew; Иванов, А. Н.; Jungvid, Hans; Galaev, Igor Yu.; James, Stuart L.; Mikhalovsky, Sergey V.

doi:10.1039/c6bm00133e

Cited by 18 publications

(15 citation statements)

References 30 publications

(34 reference statements)

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“…In this study, we investigated the bioactivity of nanobioglass on bone regeneration by fabricating bioglass-embedded methacrylated gelatin-based cryogel. To the best of our knowledge, there has been no prior report on methacrylated gelatin-based cryogel with bioglass—which would bring together the synergistic effect of cryogel, ECM for cell proliferation and host cell infiltration [16,39,40], and bioglass as a bioceramic—for enhancement of mineralization and osteogenic differentiation. We evaluated the mechanical strength, internal structure, cytotoxicity, and osteogenic differentiation ability and further carried out bone regeneration study in mouse cranial defect model.…”

Section: Introductionmentioning

confidence: 99%

Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects

et al. 2018

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Cryogels have recently gained interest in the field of tissue engineering as they inherently possess an interconnected macroporous structure. Considered to be suitable for scaffold cryogel fabrication, methacrylated gelatin (GelMA) is a modified form of gelatin valued for its ability to retain cell adhesion site. Bioglass nanoparticles have also attracted attention in the field due to their osteoinductive and osteoconductive behavior. Here, we prepare methacrylated gelatin cryogel with varying concentration of bioglass nanoparticles to study its potential for bone regeneration. We demonstrate that an increase in bioglass concentration in cryogel leads to improved mechanical property and augmented osteogenic differentiation of mesenchymal cells during in vitro testing. Furthermore, in vivo testing in mice cranial defect model shows that highest concentration of bioglass nanoparticles (2.5 w/w %) incorporated in GelMA cryogel induces the most bone formation compared to the other tested groups, as studied by micro-CT and histology. The in vitro and in vivo results highlight the potential of bioglass nanoparticles incorporated in GelMA cryogel for bone regeneration.

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Section: Introductionmentioning

confidence: 99%

Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects

et al. 2018

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“…Since typical internal structure of cryogels exhibits a pattern of interconnected macropores with diameters up to 100 or 200 μm, their permeable network may facilitate immobilization and/or molecular recognition of high molecular weight analytes. The large pores accommodate not only biopolymers but also biological cells that can be visualized by immunofluorescent staining and confocal laser scanning microscopy [9, 10, 11]. Regarding manufacturing of thin, flat translucent cryogels, the question arises if these can be made as monolithic and mechanically transferable porous films, and the present work gives a positive answer.…”

Section: Introductionmentioning

confidence: 88%

Thin poly(vinyl alcohol) cryogels: reactive groups, macropores and translucency in microtiter plate assays

Ivanov

Ljunggren

2019

Heliyon

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Thin macroporous poly(vinyl alcohol) (PVA) hydrogels were produced by cross-linking of PVA in a semi-frozen state with glutaraldehyde (GA) on glass slides or in the wells of microtiter plates. The 100-130 μm-thick gels were mechanically transferable, squamous translucent films with a high porosity of 7.2 ± 0.3 mL/g dry PVA i.e. similar to larger cylindrical PVA monoliths of the same composition. Additional treatment of the gels with 1% GA increased the aldehyde group content from 0.7 to 2.4 μmol/mL as estimated using dinitrophenylhydrazine (DNPH) reagent. Translucency of the gels allowed registration of UV-visible spectra of the DNPH-stained films. The catalytic activity of trypsin covalently immobilized on thin gels in the microtiter plates was estimated with chromogenic substrate directly in the wells, and indicated that the amount of protein immobilized was at least 0.34 mg/mL gel. Human immunoglobulin G (IgG) immobilized on thin gels at 0.1–10 mg/mL starting concentrations could be detected in a concentration-dependent manner due to recognition by anti-human rabbit IgG conjugated with peroxidase and photometric registration of the enzymatic activity. The results indicate good permeability of the hydrogel pores for macromolecular biospecific reagents and suggest applications of thin reactive PVA hydrogels in photometric analytical techniques.

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“…High migration of human dermal fibroblast by elevated fibrinogen concentration [89] Gelatin-fibrinogen cryogel Reproduction of fibroblasts and migration of them within the scaffolds [90] Chitosan-gluconic acid conjugate/poly(vinyl alcohol) cryogels…”

Section: Scaffolds Outcome Referencementioning

confidence: 99%

Biomedical Applications of Polymeric Cryogels

et al. 2019

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The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over traditional gel carriers in the field of biochromatography and related biomedical applications. These matrices nearly mimic the three-dimensional structure of native tissue extracellular matrix. In addition, mechanical, osmotic and chemical stability of cryogels make them attractive polymeric materials for the construction of scaffolds in tissue engineering applications and in vitro cell culture, separation materials for many different processes such as immobilization of biomolecules, capturing of target molecules, and controlled drug delivery. The low mass transfer resistance of cryogel matrices makes them useful in chromatographic applications with the immobilization of different affinity ligands to these materials. Cryogels have been introduced as gel matrices prepared using partially frozen monomer or polymer solutions at temperature below zero. These materials can be produced with different shapes and are of interest in the therapeutic area. This review highlights the recent advances in cryogelation technologies by emphasizing their biomedical applications to supply an overview of their rising stars day to day.

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An in vitro evaluation of fibrinogen and gelatin containing cryogels as dermal regeneration scaffolds

Cited by 18 publications

References 30 publications

Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects

Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects

Thin poly(vinyl alcohol) cryogels: reactive groups, macropores and translucency in microtiter plate assays

Biomedical Applications of Polymeric Cryogels

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