An in vitro cytotoxicity study of 5-fluorouracil encapsulated chitosan/gold nanocomposites towards MCF-7 cells

Nivethaa, E. A. K.; Dhanavel, S.; Narayanan, Vijaykrishnan; Vasu, Chenthamarakshan; Stephen, A.

doi:10.1039/c4ra11615a

Cited by 89 publications

(41 citation statements)

References 48 publications

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“…Both signals had higher intensity after incubation the mycelium with Au(III) ions, but the band of amide I was shifted to 1647 cm −1 , similarly to the weak intensive band at 1335 cm −1 confirmed v (C − N) presence of amide III . The shift of amide I signal might be connected with the neutralization of the protonated in the acidic condition

{NH}_{3}^{+}

group to receive NH 2 rest …”

Section: Resultsmentioning

confidence: 86%

Biomineralization of gold by Mucor plumbeus: The progress in understanding the mechanism of nanoparticles’ formation

Maliszewska

Tylus

Chęcmanowski

et al. 2017

Biotechnology Progress

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This contribution describes the deposition of gold nanoparticles by microbial reduction of Au(III) ions using the mycelium of Mucor plumbeus. Biosorption as the major mechanism of Au(III) ions binding by the fungal cells and the reduction of them to the form of Au(0) on/in the cell wall, followed by the transportation of the synthesized gold nanoparticles to the cytoplasm, is postulated. The probable mechanism behind the reduction of Au(III) ions is discussed, leading to the conclusion that this process is nonenzymatic one. Chitosan of the fungal cell wall is most likely to be the major molecule involved in biomineralization of gold by the mycelium of M. plumbeus. Separation of gold nanoparticles from the cells has been carried out by the ultrasonic disintegration and the obtained nanostructures were characterized by UV-vis spectroscopy and transmission electron micrograph analysis. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1381-1392, 2017.

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{NH}_{3}^{+}

group to receive NH 2 rest …”

Section: Resultsmentioning

confidence: 86%

Biomineralization of gold by Mucor plumbeus: The progress in understanding the mechanism of nanoparticles’ formation

Maliszewska

Tylus

Chęcmanowski

et al. 2017

Biotechnology Progress

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“…The viability of the cell population was more than 60%, even after 48 h exposure to the highest concentration of CS-PPy NCs (500 μg/mL), indicating a very low cytotoxicity and good biocompatibility for the CS-PPy NCs. Nivethaa et al [41] in 2015 used chitosan/gold nanocomposite with encapsulation of 5-FU. They exhibit good antiproliferative activity towards MCF-7 cells while being non-toxic to the surrounding non-carcinogenic cells.…”

Section: Resultsmentioning

confidence: 99%

Effect of Gold Nanocomposite on the Cytotoxicity of Human Cancer Cell Lines

Abaza¹,

Mahmoud²,

Elsheikh³

2018

JPP

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Gold nanoparticles (AuNPs) have a biological property that have attracted significant attention in this decade, especially in the field of biomedical application with great therapeutic potential. Additionally, the chitosan molecule appears to be a suitable polymeric complex used in this field. The aim of the current study is to explores the cytotoxicity and the IC 50 value of Au-doped chitosan-poly (vinyl alcohol) (Cs/PVA/Au) nanocomposite that was developed by gamma irradiation with promising anticancer activity. The anti-cancer activity of the prepared nanocomposites was demonstrated in human liver cancer cell line HEPG2 and MCF7 breast cancer cell lines. It has significant effects against both cancer cell line. Concluding that, the future nanomedicine will be impacted greatly by the collaboration of biomedical research and developing nanoparticle therapy in the right directions, which will improve the outcome of cancer patients. It is expected that the next decade will reveal real potential for metal nanoparticles to cross the regulatory barrier into clinical use as effective therapeutics.

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“…The X-ray powder diffraction (XRD) spectrum of 5-FU shows the characteristic diffraction peak of the crystalline drug at 28.26° (40). The halo pattern in the diffractogram of chitosan (13°, 19.8°) (41) is indicative of its semi-crystalline nature (42), while the absence of diffraction peaks in the pattern of sodium alginate is indicative of its amorphous nature. No characteristic peaks of crystalline 5-FU were detected in the diffractograms of the alginate bead formulations, suggesting either the presence of the drug in an amorphous state or a drug concentration below the detection limit of the instrument.…”

Section: X-ray Powder Diffractionmentioning

confidence: 99%

Controlled Release of 5-Fluorouracil from Alginate Beads Encapsulated in 3D Printed pH-Responsive Solid Dosage Forms

et al. 2018

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Three-dimensional printing is being steadily deployed as manufacturing technology for the development of personalized pharmaceutical dosage forms. In the present study, we developed a hollow pH-responsive 3D printed tablet encapsulating drug loaded non-coated and chitosan-coated alginate beads for the targeted colonic delivery of 5-fluorouracil (5-FU). A mixture of Eudragit® L100-55 and Eudragit® S100 was fabricated by means of hot-melt extrusion (HME) and the produced filaments were printed utilizing a fused deposition modeling (FDM) 3D printer to form the pH-responsive layer of the tablet with the rest comprising of a water-insoluble poly-lactic acid (PLA) layer. The filaments and alginate particles were characterized for their physicochemical properties (thermogravimetric analysis, differential scanning calorimetry, X-ray diffraction), their surface topography was visualized by scanning electron microscopy and the filaments' mechanical properties were assessed by instrumented indentation testing and tensile testing. The optimized filament formulation was 3D printed and the structural integrity of the hollow tablet in increasing pH media (pH 1.2 to pH 7.4) was assessed by means of time-lapsed microfocus computed tomography (μCT). In vitro release studies demonstrated controlled release of 5-FU from the alginate beads encapsulated within the hollow pH-sensitive tablet matrix at pH values corresponding to the colonic environment (pH 7.4). The present study highlights the potential of additive manufacturing in fabricating controlled-release dosage forms rendering them pertinent formulations for further in vivo evaluation.

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An in vitro cytotoxicity study of 5-fluorouracil encapsulated chitosan/gold nanocomposites towards MCF-7 cells

Abstract: Schematic outlining the synthesis of 5FU loaded CS/Au nanocomposite and its application.

Cited by 89 publications

References 48 publications

Biomineralization of gold by Mucor plumbeus: The progress in understanding the mechanism of nanoparticles’ formation

Biomineralization of gold by Mucor plumbeus: The progress in understanding the mechanism of nanoparticles’ formation

Effect of Gold Nanocomposite on the Cytotoxicity of Human Cancer Cell Lines

Controlled Release of 5-Fluorouracil from Alginate Beads Encapsulated in 3D Printed pH-Responsive Solid Dosage Forms

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