An In Vitro Comparative Study of Multisource Derived Human Mesenchymal Stem Cells for Bone Tissue Engineering

Zhang, Yunpeng; Xing, Yingjie; Jia, Linglu; Ji, Yongming; Zhao, Bin; Yang, Wen; Xu, Xin

doi:10.1089/scd.2018.0119

Cited by 86 publications

(85 citation statements)

References 46 publications

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“…Referring to cell sources, ASCs and BM-MSCs present similar absolute values, that are slightly superior to DPSCs values but no signi cant. Interestingly, this pattern in ALP activity also appears in recent studies that compare multisources derived MSCs seeded on plastic surfaces, [20,21] although these studies obtained signi cant differences. The absence of signi cance in our results could be due to the osteoinductive properties that both CaPs present, [44,45] which the plastic surface lacks.…”

Section: Discussionsupporting

confidence: 58%

“…[15] Considering the osteogenic potential as a property in uenced by the tissue, several comparative studies of MSCs derived from various sources on different surfaces have been published. [16][17][18][19][20][21] Despite these, a clari cation is still needed regarding the in uence of the cell source and surface in the osteogenic potential. Osteogenic potential maximization in a context of the bone-regeneration process would be an important step.…”

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confidence: 99%

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Dental Pulp Stem Cells: an Alternative Source of Mesenchymal Stem Cells with Potential Osteogenic Regenerative Capacities

Mucientes¹,

Herranz²,

Moro³

et al. 2020

Preprint

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Background: Bone innate ability to repair without scaring is surpassed by major bone damage. Current gold-standard strategies do not achieve a full recovery of the bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells, like mesenchymal stem cells (MSCs), and bioactive ceramic scaffolds, like calcium phosphate-based (CaPs), are promising. Biological properties of the MSCs, including osteogenic potential, are influenced by the tissue source. The aim of this study is to define the MSC source and construct (MSC and scaffold combination) most interesting for its clinical application in the context of bone regeneration.Methods: MSCs of 9 healthy donors were isolated from adipose tissue, bone marrow and dental pulp. MSCs were cultured both on plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate) to compare their biological features: proliferation rate, osteogenic potential, cell viability and activity, ability to colonize the CaPs and ALP activity. Results: iTRAQ results generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. On plastic, MSCs isolated from dental pulp (DPSCs) were the MSCs with the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs are the MSCs with the greatest capacity to colonize bioceramics. Furthermore, results show a trend for DPSCs are the MSCs with the most robust increase in the ALP activity.Conclusion: Based in our results, we propose DPSCs as a suitable MSCs for bone/dental regeneration cell-based strategies.

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Section: Discussionsupporting

confidence: 58%

mentioning

confidence: 99%

Dental Pulp Stem Cells: an Alternative Source of Mesenchymal Stem Cells with Potential Osteogenic Regenerative Capacities

Mucientes¹,

Herranz²,

Moro³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In particular, in bone tissue engineering field, several cells have been exploited such as adipose-derived stem cells (ADSCs) or birth-associated perinatal tissue umbilical cord (UMSCs). Another big family of cells that can be used is the one of dental origins, such as dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), gingival MSCs (GMSCs), and dental follicle stem cells (DFSCs) [23]. Some studies are also employing jaw bone mesenchymal stem cells (JBMSCs) [24], amnion mesenchymal stem cells (AMSCs) [25], endometrial stem cells (EnSCs) [26].…”

Section: Cells Sourcementioning

confidence: 99%

Natural and Synthetic Polymers for Bone Scaffolds Optimization

et al. 2020

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Bone tissue is the structural component of the body, which allows locomotion, protects vital internal organs, and provides the maintenance of mineral homeostasis. Several bone-related pathologies generate critical-size bone defects that our organism is not able to heal spontaneously and require a therapeutic action. Conventional therapies span from pharmacological to interventional methodologies, all of them characterized by several drawbacks. To circumvent these effects, tissue engineering and regenerative medicine are innovative and promising approaches that exploit the capability of bone progenitors, especially mesenchymal stem cells, to differentiate into functional bone cells. So far, several materials have been tested in order to guarantee the specific requirements for bone tissue regeneration, ranging from the material biocompatibility to the ideal 3D bone-like architectural structure. In this review, we analyse the state-of-the-art of the most widespread polymeric scaffold materials and their application in in vitro and in vivo models, in order to evaluate their usability in the field of bone tissue engineering. Here, we will present several adopted strategies in scaffold production, from the different combination of materials, to chemical factor inclusion, embedding of cells, and manufacturing technology improvement.

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“…Over the past few decades, rapid development in the field of dental research led to a promising prospect in stem cell–based tissue regeneration and cell therapies for infection‐related diseases. In addition to the oral and maxillofacial area, data have shown that PDLSCs might be a good potential alternative to BMMSCs for bone tissue engineering . Therefore, it is of great importance to have a better understanding of the behavior of PDLSCs exposed to bacterial toxins and reveal the impact of E. coli LPS on osteogenesis and potential molecular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide from Escherichia coli stimulates osteogenic differentiation of human periodontal ligament stem cells through Wnt/β‐catenin–induced TAZ elevation

Xing

Zhang

Jia

et al. 2018

Molecular Oral Microbiology

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Human periodontal ligament stem cells (PDLSCs), a type of dental tissue–derived mesenchymal stem cells (MSCs), can be clinically applied in periodontal tissue regeneration to treat periodontitis, which is initiated and sustained by bacteria. Lipopolysaccharide (LPS), the major component of the outer membrane of gram‐negative bacteria, is a pertinent deleterious factor in the oral microenvironment. The aim of this study was to investigate the effect of LPS on the proliferation and osteogenic differentiation of PDLSCs, as well as the mechanisms involved. Proliferation and osteogenic differentiation of PDLSCs were detected under the stimulation of Escherichia coli–derived LPS. The data showed that E. coli–derived LPS did not affect the proliferation, viability, and cell cycle of PDLSCs. Furthermore, it promoted osteogenic differentiation with the activation of TAZ. Lentivirus‐mediated depletion of TAZ (transcriptional activator with a PDZ motif) was used to determine the role of TAZ on LPS‐induced enhancement of osteogenesis. PDLSCs cultured in osteogenic media with or without LPS and DKK1 (Wnt/β‐catenin pathway inhibitor) were used to determine the regulatory effect of Wnt signaling. We found that TAZ depletion offset LPS‐induced enhancement of osteogenesis. Moreover, treatment with DKK1 offset LPS‐induced TAZ elevation and osteogenic promotion. In conclusion, E. coli–derived LPS promoted osteogenic differentiation of PDLSCs by fortifying TAZ activity. The elevation and activation of TAZ were mostly mediated by the Wnt/β‐catenin pathway. PDLSC‐governed alveolar bone tissue regeneration was not necessarily reduced under bacterial conditions and could be modulated by Wnt and TAZ.

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An In Vitro Comparative Study of Multisource Derived Human Mesenchymal Stem Cells for Bone Tissue Engineering

Cited by 86 publications

References 46 publications

Dental Pulp Stem Cells: an Alternative Source of Mesenchymal Stem Cells with Potential Osteogenic Regenerative Capacities

Dental Pulp Stem Cells: an Alternative Source of Mesenchymal Stem Cells with Potential Osteogenic Regenerative Capacities

Natural and Synthetic Polymers for Bone Scaffolds Optimization

Lipopolysaccharide from Escherichia coli stimulates osteogenic differentiation of human periodontal ligament stem cells through Wnt/β‐catenin–induced TAZ elevation

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