An in-vitro comparative evaluation of quantitative release of transforming growth factor β-1 from dentin upon the action of endodontic irrigants, medicaments, ultrasonic activation, and low-level laser irradiation

Akhila, Anilkumar; Singh, Vinod P.; Varma, Kerala; Vasudevan, S; Sukhithasri, V.; Sasikumar, Salu

doi:10.4103/amjm.amjm_11_21

Cited by 3 publications

(2 citation statements)

References 60 publications

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“…Moreover, the XP-endo Finisher has improved irrigation dynamics as the file exhibits increased flexibility and enhanced expansion capacity which leads to the improvement of the penetration of the irrigant into the dentinal tubules [ 45 ]. The increase in the TGF-β1 release in the EDTA/DW/XP group is in accordance with studies that showed that irrigation activation increases the growth factor release from dentin [ 7 , 13 , 34 , 47 ]. XP-endo Finisher showed higher results when compared with passive ultrasonic irrigation during activation of the irrigants on smear layer removal and dentinal tubules opening as well as increased bacterial removal [ 48 , 49 ].…”

Section: Discussionsupporting

confidence: 90%

Impact of using XP-endo finisher and nanobubble water during EDTA dentin conditioning on TGF-β1 release in regenerative endodontic procedures

Hanafy,

Abdella Ahmed,

Salem

2024

BMC Oral Health

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Introduction Transforming Growth Factor-Beta 1 (TGF-β1) plays a crucial role in the success of Regenerative Endodontic Procedures (REPs) as they directly impact the proliferation and differentiation of stem cells. TGF-β1 is released by conditioning of the dentin matrix using 17% EDTA. EDTA was found to have deleterious effects on dentin especially in immature teeth with fragile dentin walls. Decreasing the irrigation time was reported to decrease these effects. Accordingly, enhancement and activation of the EDTA solution to maintain its efficiency in TGF-β1 release from dentin and thus compensating the reduction in irrigation time was employed. EDTA solution was enhanced by adding Nanobubble (NB) water which contains oxygen filled cavities less than 200 nm in diameter. Additionally, EDTA was activated with XP-endo Finisher rotary file. The aim of this study was to assess the impact of NB enhancement and/or XP-endo Finisher activation of the EDTA solution on the TGF-β1 release from dentin. Methods Fifty standardized root segments with open apex were allocated to two main groups according to whether EDTA was enhanced with NB water or not, and within each group whether XP-endo Finisher activation was used or not in addition to a Negative Control group. The concentration of the released TGF-β1 in the root canal was measured using enzyme-linked immunosorbent assay (ELISA). The statistical analysis was done using the Shapiro- Wilk, Kolmogorov Smirnov, ANOVA and Post-hoc Tukey tests. Results All groups released a considerable amount of TGF-β1 with the highest values in the EDTA/NB/XP group, followed by EDTA/NB, EDTA/DW/XP, EDTA/DW and Negative Control groups respectively. Conclusions The results of this study suggest that NBs can promote the success of REPs since it revealed a significant increase in the TGF-β1 release following its use in the enhancement of the EDTA solution. A comparable effect was obtained by XP-endo finisher activation of the EDTA solution. The combined use of NBs and XP-endo Finisher can be a promising addition in REPs. Accordingly, Enhancement and activation of the EDTA solution may compensate decreasing the EDTA irrigation time attempted to avoid the deleterious effect of EDTA on dentin.

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Section: Discussionsupporting

confidence: 90%

Impact of using XP-endo finisher and nanobubble water during EDTA dentin conditioning on TGF-β1 release in regenerative endodontic procedures

Hanafy,

Abdella Ahmed,

Salem

2024

BMC Oral Health

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“…SCAPs express the TGF-β receptor 1 [10], suggesting they can receive regulation from TGF-β ligands. Secreted TGF-β1 and TGF-β2 are embedded in the dentin during tooth development, and they can release from the dentin during endodontic procedures, such as root canal therapy [41][42][43]. Studies have demonstrated that TGF-β1 can inhibit the proliferation, differentiation, and mineralization of SCAPs in dose-dependent and Smad3-dependent manners [41,44].…”

Section: Transforming Growth Factor-b Superfamilymentioning

confidence: 99%

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Liu,

Gao,

2023

Biomedicines

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Dental diseases occurring on young permanent teeth usually lead to the premature arrest of tooth root development. Sustained tooth root elongation is necessary to achieve the goal of long-term preservation of affected teeth. To this end, stem cell-based regenerative endodontic treatment has been regarded as one of the most promising strategies for treating young permanent teeth with pulp and periapical infections. Endogenous stem cells residing in the apical papilla, named stem cells from the apical papilla (SCAPs), have been intensively investigated due to their critical roles in pulp regeneration and root redevelopment. The present review summarizes advances in the field of SCAPs studies and discusses the challenges that need to be further addressed.

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Citric Acid Conditioning as an Alternative to EDTA for Growth Factors Release and Stem Cell Response in Regenerative Endodontics: A Systematic Review of In Vitro Studies

Reis-Prado,

Toledo,

Nunes

et al. 2024

Journal of Endodontics

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An in-vitro comparative evaluation of quantitative release of transforming growth factor β-1 from dentin upon the action of endodontic irrigants, medicaments, ultrasonic activation, and low-level laser irradiation

Cited by 3 publications

References 60 publications

Impact of using XP-endo finisher and nanobubble water during EDTA dentin conditioning on TGF-β1 release in regenerative endodontic procedures

Impact of using XP-endo finisher and nanobubble water during EDTA dentin conditioning on TGF-β1 release in regenerative endodontic procedures

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Citric Acid Conditioning as an Alternative to EDTA for Growth Factors Release and Stem Cell Response in Regenerative Endodontics: A Systematic Review of In Vitro Studies

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