An in vitro assay based on surface plasmon resonance to predict the in vivo circulation kinetics of liposomes

Crielaard, Bart J.; Yousefi, Afrouz; Schillemans, JP; Vermehren, Charlotte; Buyens, Kevin; Braeckmans, Kevin; Lammers, Twan; Storm, Gert

doi:10.1016/j.jconrel.2011.07.023

Cited by 31 publications

(29 citation statements)

References 63 publications

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“…Anionic and cationic charged nanoparticles favour proteins with pI o 5.5 and pI 4 5.5 respectively. 61,62 The hydrophobicity of the nanoparticle also exerts effects on the protein corona by stabilizing protein binding and encouraging opsonin interactions. 7,28 As the size and surface charge change, nanoparticles may begin to repel serum protein, actively bind proteoglycans, 44 or form corona with varying abundances of high density lipoprotein (HDL), low density lipoprotein (LDL), and acute phase proteins.…”

Section: The Relationship Between the Synthetic And Biological Identimentioning

confidence: 99%

“…Serum protein adsorption to nanoparticles coated with targeting ligands may block bio-recognition molecules from binding to the target receptor through steric effects. 21,62 Others have noted that the greatest reduction of non-specific serum protein adsorption occurs at an optimal surface density, in which the PEG conformation on the nanoparticle surface is in an intermediate conformation between the ''mushroom'' to ''brush'' configuration. The uncoated surface can adsorb the serum protein and the protein corona can mask the nanoparticle surface by sterically hindering receptor access to targeting ligands, and inhibit nanoparticle targeting capabilities by decreasing the probability of a favourable receptor-ligand binding event.…”

Section: How Does Nanoparticle-blood Interaction Influence Tumour Tarmentioning

confidence: 99%

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Nanoparticle–blood interactions: the implications on solid tumour targeting

et al. 2015

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Nanoparticles are suitable platforms for cancer targeting and diagnostic applications. Typically, less than 10% of all systemically administered nanoparticles accumulate in the tumour. Here we explore the interactions of blood components with nanoparticles and describe how these interactions influence solid tumour targeting. In the blood, serum proteins adsorb onto nanoparticles to form a protein corona in a manner dependent on nanoparticle physicochemical properties. These serum proteins can block nanoparticle tumour targeting ligands from binding to tumour cell receptors. Additionally, serum proteins can also encourage nanoparticle uptake by macrophages, which decreases nanoparticle availability in the blood and limits tumour accumulation. The formation of this protein corona will also increase the nanoparticle hydrodynamic size or induce aggregation, which makes nanoparticles too large to enter into the tumour through pores of the leaky vessels, and prevents their deep penetration into tumours for cell targeting. Recent studies have focused on developing new chemical strategies to reduce or eliminate serum protein adsorption, and rescue the targeting potential of nanoparticles to tumour cells. An in-depth and complete understanding of nanoparticle-blood interactions is key to designing nanoparticles with optimal physicochemical properties with high tumour accumulation. The purpose of this review article is to describe how the protein corona alters the targeting of nanoparticles to solid tumours and explains current solutions to solve this problem.

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Section: The Relationship Between the Synthetic And Biological Identimentioning

confidence: 99%

Section: How Does Nanoparticle-blood Interaction Influence Tumour Tarmentioning

confidence: 99%

Nanoparticle–blood interactions: the implications on solid tumour targeting

et al. 2015

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“…One strategy to increase intracellular uptake is to attach targeting ligands to non-viral vectors to increase receptor-mediated endocytosis. These ligands mostly target nutrient uptake receptors such as transferrin, folate, and low-density lipoprotein receptors (LDL) 12,13 . Although this leads to improvement in cellular uptake and gene delivery, other more powerful strategies should be taken into consideration.…”

Section: Changes In the Composition Of Lipoplexes Or Polyplexes Upon mentioning

confidence: 99%

Nucleic Acid Delivery: The Missing Pieces of the Puzzle?

2012

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Conspectus The ability of gene or RNA interference (RNAi) delivery to increase or decrease virtually any protein in a cell opens the path for cures to most diseases that afflict humans. However, their high molecular weight, anionic nature, and instability in the presence of enzymes, pose major obstacles to nucleic acid delivery and frustrates their use as human therapies. This Account describes current ideas on the mechanisms in non-viral nucleic acid delivery and how lipidic and polymeric carriers overcome some of the critical barriers to delivery. A multitude of polymeric and lipidic vectors have been developed over the last 20 years, only a small fraction of them have progressed into clinical trials. Given that none of these vectors has received FDA approval, indicates that the current vectors do not yet have suitable properties for effective in vivo nucleic acid delivery. Nucleic acid delivery is a multistep process and inefficiencies at any stage result in a dramatic decrease in gene delivery or gene silencing. Despite this, the majority of studies investigating synthetic vectors focus solely on optimization of endosomal escape. A small number of studies address how to improve uptake via targeted delivery. A smaller fraction examine the intracellular fate of the delivery systems and nucleic acid cargo. The internalization of genes into the cell nucleus remains an inefficient and mysterious process. In the case of DNA delivery, strategies to increase and accelerate the migration of DNA through the cytoplasm and transport it through the nuclear membrane are required. The barriers to siRNA delivery are fewer: siRNA is more readily released from the carrier, siRNA is more resistant to enzymatic degradation and the target is in the cytoplasm; hence, siRNA delivery systems are becoming a clinical reality. With regard to siRNA therapy, the exact cytoplasmic location of RISC formation and activity is unknown. This makes specific targeting of the RISC for more efficient siRNA delivery difficult. Furthermore, identifying the factors favoring the binding of siRNA to Ago-2 and understanding how the half-life of siRNA and Ago-2/siRNA complex in the cytoplasm can be modulated without interfering with RISC functions that are essential for normal cell activity could increase siRNA delivery efficiency. In this manuscript we concisely review the current synthetic vectors and for a few of these, propose alternative strategies. We suggest how certain cellular mechanisms might be exploited to improve gene transfection and silencing. Finally, we raise the question if some carriers are delivering the siRNA to cells capable of repackaging the siRNA into exosomes. The exosomes would then transport the siRNA into a subsequent population of cells where the siRNA effect is manifest. This piggy-back mechanism may be responsible for reported deep tissue siRNA effects using certain carriers.

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“…A higher sensitivity is expected for the latter option due to the higher mass of the NP providing that the binding of the proteins to the sensor surface does not alter their typical configuration regarding protein/NP interactions. Previous SPR studies on the interactions of nanovehicles with proteins of the bloodstream include the investigation of the corona formation by employing serum or plasma [22,23] and the interaction of nanoparticles with individual proteins known to adsorb onto the surface of nanocarriers [24], an approach that has been considered for the present experimental design.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

Ianni

Islan

Chain

et al. 2017

Journal of Nanomaterials

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The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the "protein corona" around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN.

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An in vitro assay based on surface plasmon resonance to predict the in vivo circulation kinetics of liposomes

Cited by 31 publications

References 63 publications

Nanoparticle–blood interactions: the implications on solid tumour targeting

Nanoparticle–blood interactions: the implications on solid tumour targeting

Nucleic Acid Delivery: The Missing Pieces of the Puzzle?

Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance

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