An in vitro and in silico identification of antibiofilm small molecules from seawater metaclone SWMC166 against Vibrio cholerae O1

Rajalaxmi, Murugan; Shafreen, Rajamohamed Beema; Chithiraiselvi, Karuppiah; Pandian, Shunmugiah Karutha

doi:10.1016/j.mcp.2018.03.003

Cited by 5 publications

(2 citation statements)

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“…Undirected approaches with large compound libraries are gaining traction in anti‐biofilm discovery. For instance, functional metagenomics from seawater samples are employed to identify anti‐biofilm compounds against Vibrio cholerae and P. aeruginosa , while transcriptomics and proteomics are used to study the anti‐biofilm activity of 3‐furancarboxaldehyde on Group A S treptococcus…”

Section: Development Of Anti‐biofilm Chemotherapymentioning

confidence: 99%

Demolishing the great wall of biofilms in Gram‐negative bacteria: To disrupt or disperse?

Chua

2019

Medicinal Research Reviews

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Section: Development Of Anti‐biofilm Chemotherapymentioning

confidence: 99%

Demolishing the great wall of biofilms in Gram‐negative bacteria: To disrupt or disperse?

Chua

2019

Medicinal Research Reviews

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“…Interestingly, inter-species bacterially secreted QS compounds have been also identified [9,10]. Significant efforts have been directed in recent years towards development of anti-bacterial therapeutic strategies based upon identification of antagonists or agonists in QS cascades [11,12]. Such strategies have attracted considerable interest, as they circumvent the emergence of bacterial resistance to antibiotic compounds, a major and growing challenge in anti-bacterial therapeutics [13].…”

Section: Introductionmentioning

confidence: 99%

Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites

et al. 2021

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Background Probiotic milk-fermented microorganism mixtures (e.g., yogurt, kefir) are perceived as contributing to human health, and possibly capable of protecting against bacterial infections. Co-existence of probiotic microorganisms are likely maintained via complex biomolecular mechanisms, secreted metabolites mediating cell-cell communication, and other yet-unknown biochemical pathways. In particular, deciphering molecular mechanisms by which probiotic microorganisms inhibit proliferation of pathogenic bacteria would be highly important for understanding both the potential benefits of probiotic foods as well as maintenance of healthy gut microbiome. Results The microbiome of a unique milk-fermented microorganism mixture was determined, revealing a predominance of the fungus Kluyveromyces marxianus. We further identified a new fungus-secreted metabolite—tryptophol acetate—which inhibits bacterial communication and virulence. We discovered that tryptophol acetate blocks quorum sensing (QS) of several Gram-negative bacteria, particularly Vibrio cholerae, a prominent gut pathogen. Notably, this is the first report of tryptophol acetate production by a yeast and role of the molecule as a signaling agent. Furthermore, mechanisms underscoring the anti-QS and anti-virulence activities of tryptophol acetate were elucidated, specifically down- or upregulation of distinct genes associated with V. cholerae QS and virulence pathways. Conclusions This study illuminates a yet-unrecognized mechanism for cross-kingdom inhibition of pathogenic bacteria cell-cell communication in a probiotic microorganism mixture. A newly identified fungus-secreted molecule—tryptophol acetate—was shown to disrupt quorum sensing pathways of the human gut pathogen V. cholerae. Cross-kingdom interference in quorum sensing may play important roles in enabling microorganism co-existence in multi-population environments, such as probiotic foods and the gut microbiome. This discovery may account for anti-virulence properties of the human microbiome and could aid elucidating health benefits of probiotic products against bacterially associated diseases.

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Quorum sensing inhibitors applications: A new prospect for mitigation of microbiologically influenced corrosion

Lamin

Kaksonen

Cole

et al. 2022

Bioelectrochemistry

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An in vitro and in silico identification of antibiofilm small molecules from seawater metaclone SWMC166 against Vibrio cholerae O1

Cited by 5 publications

References 43 publications

Demolishing the great wall of biofilms in Gram‐negative bacteria: To disrupt or disperse?

Demolishing the great wall of biofilms in Gram‐negative bacteria: To disrupt or disperse?

Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites

Quorum sensing inhibitors applications: A new prospect for mitigation of microbiologically influenced corrosion

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