An in-vacuum, pixelated detection system for mass spectrometric analysis and imaging of macromolecules

Jungmann, Julia H.; Smith, Donald F.; Kiss, András; MacAleese, Luke; Buijs, Ronald; Heeren, Ron M. A.

doi:10.1016/j.ijms.2013.02.010

Cited by 21 publications

(39 citation statements)

References 31 publications

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“…Thus, it is capable of providing complementary information to the positive mode measurements. This new functionality of the system was previously demonstrated on peptide and protein standards with MALDI . However, no negative mode images have been recorded thus far with a pixelated detector.…”

Section: Resultsmentioning

confidence: 99%

“…The latest development in the field of microscope mode MSI has been the introduction of pixelated detectors, such as the Medipix/Timepix detector family. [25][26][27][28][29][30] In these detectors, every pixel acts as an individual detector capable of recording the time-of-flight of the arriving ions with respect to an external trigger signal. Combined with MCPs, they offer multiplexed ion detection capabilities where every ion hit is registered by multiple pixels.…”

mentioning

confidence: 99%

“…The capabilities of the Timepix detectors were previously demonstrated for microscope mode MALDI imaging. [27][28][29][30] In earlier work, we presented the first example of microscope mode SIMS imaging with a Timepix detector. [31] The potential of this detection system for SIMS microscope mode MSI, namely superior spatial resolving power and signal-to-noise, was demonstrated.…”

mentioning

confidence: 99%

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Cluster secondary ion mass spectrometry microscope mode mass spectrometry imaging

Kiss

Smith

Jungmann

et al. 2013

Rapid Comm Mass Spectrometry

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Cluster secondary ion mass spectrometry microscope mode mass spectrometry imaging

Kiss

Smith

Jungmann

et al. 2013

Rapid Comm Mass Spectrometry

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“…The sample is irradiated with a diffused laser and specialized ion optics preserve the generated ions’ relative two-dimensional spatial localization before their detection by a positionsensitive detector. The Heeren group has developed—and is continually improving—mass microscopes that allow this detection modality [57–61]. Several types of samples have been imaged using the mass microscopes [58,62–64].…”

Section: Ms-based Approachesmentioning

confidence: 99%

Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples

Ong

Tillmaand

Makurath

et al. 2015

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Technologies to assay single cells and their extracellular microenvironments are valuable in elucidating biological function, but there are challenges. Sample volumes are low, the physicochemical parameters of the analytes vary widely, and the cellular environment is chemically complex. In addition, the inherent difficulty of isolating individual cells and handling small volume samples complicates many experimental protocols. Here we highlight a number of mass spectrometry (MS)-based measurement approaches for characterizing the chemical content of small volume analytes, with a focus on methods used to detect intracellular and extracellular metabolites and peptides from samples as small as individual cells. MS has become one of the most effective means for analyzing small biological samples due to its high sensitivity, low analyte consumption, compatibility with a wide array of sampling approaches, and ability to detect a large number of analytes with different properties without preselection. Having access to a flexible portfolio of MS-based methods allows quantitative, qualitative, untargeted, targeted, multiplexed, spatially resolved investigations of single cells and their similarly scaled extracellular environments. Combining MS with on-line and off-line sample conditioning tools, such as microfluidic and capillary electrophoresis systems, significantly increases the analytical coverage of the sample’s metabolome and peptidome, and improves individual analyte characterization / identification. Small volume assays help to reveal the causes and manifestations of biological and pathological variability, as well as the functional heterogeneity of individual cells within their microenvironments and within cellular populations.

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“…Previously, the use of the Timepix detector with other MS technologies such as secondary ion mass spectrometry (SIMS) [21] and a TOF instrument [22,23] has been demonstrated for MSI with a spatial resolution of few micrometers. In this paper, the implementation of a Timepix detector system on a QMS with a cooling octopole ion guide is presented.…”

Section: Introductionmentioning

confidence: 99%

Experimental Investigation of the 2D Ion Beam Profile Generated by an ESI Octopole-QMS System

Syed

Eijkel

Kistemaker

et al. 2014

J. Am. Soc. Mass Spectrom.

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In this paper, we have employed an ion imaging approach to investigate the behavior of ions exiting from a quadrupole mass spectrometer (QMS) system that employs a radio frequency octopole ion guide before the QMS. An in-vacuum active pixel detector (Timepix) is employed at the exit of the QMS to image the ion patterns. The detector assembly simultaneously records the ion impact position and number of ions per pixel in every measurement frame. The transmission characteristics of the ion beam exiting the QMS are studied using this imaging detector under different operating conditions. Experimental results confirm that the ion spatial distribution exiting the QMS is heavily influenced by ion injection conditions. Furthermore, ion images from Timepix measurements of protein standards demonstrate the capability to enhance the quality of the mass spectral information and provide a detailed insight in the spatial distribution of different charge states (and hence different m/z) ions exiting the QMS.

show abstract

An in-vacuum, pixelated detection system for mass spectrometric analysis and imaging of macromolecules

Cited by 21 publications

References 31 publications

Cluster secondary ion mass spectrometry microscope mode mass spectrometry imaging

Cluster secondary ion mass spectrometry microscope mode mass spectrometry imaging

Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples

Experimental Investigation of the 2D Ion Beam Profile Generated by an ESI Octopole-QMS System

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