An improvised process of isolation, purification of steviosides from <i><scp>S</scp>tevia rebaudiana </i><scp>B</scp>ertoni leaves and its biological activity

Rao, A. S.; Reddy, G. Roopa; Prasad, Edamana; Sridhar, S.; Ravikumar, Y. V. L.

doi:10.1111/j.1365-2621.2012.03134.x

Cited by 33 publications

(12 citation statements)

References 35 publications

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“…Methanol extract showed two zones on TLC plates. The methanol extract on complete non-polar compounds and greasy materials from the Stevia leaf extract, as the solvent is non-polar in nature and sweet compounds thus retained behind in the residue were partitioned into butanol from water [28,[36][37][38] . Butanol and water are immiscible solvents therefore used advantageously to remove the impurities from the residue, leaving behind the sweet compounds in the butanol layer [39,40] .…”

Section: Resultsmentioning

confidence: 99%

Extraction, Purification and Analysis of Sweet Compounds in Stevia rebaudiana Bertoni using Chromatographic Techniques

Kumari¹,

Rana²,

Sharma³

et al. 2017

pharmaceutical-sciences

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Neena, et al.: Estimation of Diterpenoid Glycosides of S. rebaudianaStevioside and rebaudioside-A are two commercially important diterpenoid glycoside natural sweeteners present in Stevia rebaudiana Bertoni. Stevioside is the major component about 300 times sweeter than sugar, while rebaudioside-A is 1.6 to 1.8 times sweeter than stevioside. Some of the modern extraction and purification techniques developed have been found to be complex and expensive. Therefore, an attempt was made to extract and purify stevioside and rebaudioside-A using simple, easy and inexpensive thinlayer chromatography and column chromatography techniques. Dried leaves were extracted with methanol followed by purification through column chromatography and further analysis was done through high performance liquid chromatography. This extraction method provided a simple and cost effective route to obtain almost pure stevioside and rebaudioside-A.

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Section: Resultsmentioning

confidence: 99%

Extraction, Purification and Analysis of Sweet Compounds in Stevia rebaudiana Bertoni using Chromatographic Techniques

Kumari¹,

Rana²,

Sharma³

et al. 2017

pharmaceutical-sciences

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“…Among the most well-known alternatives to sucrose are steviolglycosides (steviosides) [69], which are diterpene glycosides obtained from the leaves of Stevia rebaudiana Bertoni (family Asteraceae). These compounds are around 300 times sweeter than sucrose [70]. The sweetening properties of the steviol-glycosides differ from one another in sweetness and quality of the taste, so the organoleptic properties of the steviosides depend a lot on the extraction technique used to extract them from stevia leaves [70].…”

Section: Phwe Of Diterpenesmentioning

confidence: 99%

“…These compounds are around 300 times sweeter than sucrose [70]. The sweetening properties of the steviol-glycosides differ from one another in sweetness and quality of the taste, so the organoleptic properties of the steviosides depend a lot on the extraction technique used to extract them from stevia leaves [70].…”

Section: Phwe Of Diterpenesmentioning

confidence: 99%

Pressurized hot water extraction of bioactives

Plaza

Turner

2015

TrAC Trends in Analytical Chemistry

419

224

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HIGHLIGHTS  Review of pressurized hot water extraction of bioactive compounds, 2009-14  Chemical and physical properties of pressurized hot liquid water  Equipment, method optimization, applications, coupling and future prospects  Challenges with degradation and other chemical reactions during extraction ABSTRACT The purpose of this review is to give the reader a thorough background to the fundamentals and applications of pressurized hot water extraction (PHWE) for the analysis of bioactive compounds. We summarize the field in the period 2009-14, and include fundamentals of water as a solvent: equipment; method optimization; applications; coupling; and, future prospects. We highlight that solvent properties of water are tunable by changing the temperature, particularly self-ionization, dielectric constant, viscosity, diffusivity, density and surface tension. Furthermore, important aspects to consider are the risk of degradation of the analytes and other potential reactions, such as hydrolysis, caramelization and Maillard reactions that may lead to erroneous results. For the extraction of bioactive compounds, we report PHWE methods based on using water of 80-175°C and short extraction times. In conclusion, PHWE provides advantages over conventional extraction methods, such as being "greener", faster and more efficient. Why another review article on pressurized hot water extraction?Pressurized hot water extraction (PHWE) is an extraction technique that uses liquid water as extractant (extraction solvent) at temperatures above the atmospheric boiling point of water (100°C/273 K, 0.1 MPa), but below the critical point of water (374°C/647 K, 22.1 MPa) (Fig. 1). The use of PHWE in analytical chemistry started with the work in environmental analysis by Hawthorne and colleagues in the mid-1990s [1,2], and can also be referred to as subcritical water extraction (SWE), superheated water extraction and pressurized liquid extraction or accelerated solvent extraction with water as a solvent. There are a few relatively recent review articles on analytical PHWE, which the reader is recommended to read [3][4][5][6][7].The aim of this review article is to give a thorough background on the fundamental properties of water -an aspect that has been virtually overlooked in most review articles written so far about analytical PHWE. Hence, the first part of this review article concerns the fundamentals of chemical/physical properties of water and how these change with the increase in temperature, as well as how these affect the extraction performance both positively and negatively in different analytical applications.The second part deals with technical solutions of PHWE and how to conduct the experiment in practice. This technical part includes discussions on using commercially available and home-built equipment. The third part includes aspects on method optimization in PHWE. The fourth part summarizes some of the key applications and related publications mainly in the field of extraction of bioactive compounds from plants, food, ...

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“…These methods were optimized in terms of temperature, duration of the process, stability and quantity of molecules extracted, whereas the conventional methods of extraction (Soxhlet extraction, hot or cold maceration, and hydro distillation) were less desirable from both an economic and an extractive standpoint (Table 1) [31][32][33]. Table 1.…”

Section: S Rebaudiana Chemical Constituents and Extraction Proceduresmentioning

confidence: 99%

Is Stevia rebaudiana Bertoni a Non Cariogenic Sweetener? A Review

et al. 2015

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Stevia rebaudiana Bertoni is a small perennial shrub of the Asteraceae (Compositae) family that is native to South America, particularly Brazil and Paraguay, where it is known as "stevia" or "honey leaf" for its powerful sweetness. Several studies have suggested that in addition to their sweetness, steviosides and their related compounds, including rebaudioside A and isosteviol, may offer additional therapeutic benefits. These benefits include anti-hyperglycaemic, anti-hypertensive, anti-inflammatory, anti-tumor, anti-diarrheal, diuretic, and immunomodulatory actions. Additionally, critical analysis of the literature supports the anti-bacterial role of steviosides on oral bacteria flora. The aim of this review is to show the emerging results regarding the anti-cariogenic properties of S. rebaudiana Bertoni. Data shown in the present paper provide evidence that stevioside extracts from S. rebaudiana are not cariogenic. Future research should be focused on in vivo studies to evaluate the effects on dental caries of regular consumption of S. rebaudiana extract-based products.

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An improvised process of isolation, purification of steviosides from Stevia rebaudiana Bertoni leaves and its biological activity

Cited by 33 publications

References 35 publications

Extraction, Purification and Analysis of Sweet Compounds in Stevia rebaudiana Bertoni using Chromatographic Techniques

Extraction, Purification and Analysis of Sweet Compounds in Stevia rebaudiana Bertoni using Chromatographic Techniques

Pressurized hot water extraction of bioactives

Is Stevia rebaudiana Bertoni a Non Cariogenic Sweetener? A Review

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