An improved transformation system for Lombardy poplar (<i>Populus nigra</i>var.<i>italica</i>)

Nishiguchi, Mitsuru; Yoshida, Kazumasa; Mohri, Takeshi; Igasaki, Tomohiro; Shinohara, Kenji

doi:10.1007/s10310-006-0203-1

Cited by 10 publications

(13 citation statements)

References 22 publications

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“…In agreement with a report [11], we found that a combination of 0.5 mg/L IBA and 0.02 mg/L NAA was the most effective dose for root development in Lombardy poplar (Figure 1(C) and 1(D)), although 0.5 mg/L IBA alone could induce roots in 50% of the regenerated shoots ( Table 2). Doubling the concentration of IBA and NAA in the medium did not improve the root induction efficiency ( Table 2).…”

Section: Root Inductionsupporting

confidence: 80%

“…Then, four weeks old calli developed in CIM were repeatedly subcultured in SIM containing 2 mg/L trans-zeatin and 0.2 mg/L benzylaminopurine (BA), followed by re-transfer to root induction medium (RIM). In later by Nishiguchi et al [11], much simple regeneration method had been developed for Lombardy poplar regeneration, without callus formation. But, still they relied on pre incubation of explants in CIM (0.5 mg/L 2,4-D and 1 mg/L BA) for 6 days.…”

Section: Introductionmentioning

confidence: 99%

“…These methods consists of two steps of tissue culture stages; first, stem or leaf explants were incubated on the medium containing high concentration of plant hormone auxin (in most case, it is called callus inducing medium, CIM) for several days then transferred to shoot induction medium (SIM) that dominantly contains plant hormone cytokinin. The system is also available for Agrobacterium-mediated gene transformation [10,11,15]. In an early study of Lombardy poplar regeneration by Mohri et al [15], 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) was added to CIM for calli induction.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro plant regeneration of Lombardy poplar [11] or its close hybrids [10,[12][13][14] was achieved from leaf or stem explants. These methods consists of two steps of tissue culture stages; first, stem or leaf explants were incubated on the medium containing high concentration of plant hormone auxin (in most case, it is called callus inducing medium, CIM) for several days then transferred to shoot induction medium (SIM) that dominantly contains plant hormone cytokinin.…”

Section: Introductionmentioning

confidence: 99%

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An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Biswas¹,

Mohri²,

Kogawara³

et al. 2012

AJPS

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We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.

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Section: Root Inductionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Biswas¹,

Mohri²,

Kogawara³

et al. 2012

AJPS

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“…For example, transformation protocols have been developed for P. alba (Okumura et al, 2006), P. tremula (Fladung et al, 1997;Tzfira et al, 1997), P. tremuloides (Cseke et al, 2007), P. tremula × P. alba (Leple et al, 1992), P. tremula × P. tremuloides (Fladung et al, 1997), Populus canescens × P. grandidentada and P. tremuloides × P. davidiana (Dai et al, 2003). Successful transformation have been reported for species and hybrids from sections Aigeiros (cottonwood) and Tacamahaca (balsam poplar) including P. deltoides, P. trichocarpa "Nisqually-1" Song et al, 2006), P. trichocarpa × P. deltoides (De Block, 1990;Han et al, 2000), P. deltoides × P. nigra (Heuchelin et al, 1997), P. alba × P. grandidentata (Fillatti et al, 1987) and P. nigra × P. trichocarpa (McCown et al, 1991), P. sieboldii × P. grandidentata (Matsunaga et al, 2002), P. nigra "Italica" (Nishiguchi et al, 2006) and P. ciliata (Thakur et al, 2005). The most widely used transformation "laboratory rat," has been the Populus tremula × alba hybrid clone known as INRA 717-1B4 (Leple et al, 1992).…”

Section: Transformation Methods In Populusmentioning

confidence: 99%

Transformation as a Tool for Genetic Analysis in Populus

Busov

Strauss

Pilate³

2009

Genetics and Genomics of Populus

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We summarize the outlook for using transformation as a genetic tool in Populus. Transformation approaches avoid the major obstacle to performing genetics experiments in trees -namely long generation cycles and the difficulty of inbreeding to reveal loss of function alleles. Dominant transgenic alleles allow modifications in gene function to be readily observed in primary transformants. Although transformation has been mainly used for reverse genetics (where the gene sequence of interest is known), transgenic mutagenesis approaches such as activation tagging and gene/enhancer traps have also been shown to enable forward genetics (where the phenotype, not the gene, is known). We outline challenges and needs for more efficient use of transformation tools. These include expansion of the transformation toolbox (e.g., promoters, vectors, targeting), and improved ability to conduct field trials to study gene function in native and plantation environments (in spite of regulatory obstacles). Because of the power of transformation, it will remain a major genetic research tool for dissection of gene function in Populus for many years to come. It is the key biological attribute that makes poplar the most powerful model organism for genetic analysis of woody plant growth, adaptation, and development.

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Populus alba cationic cell-wall-bound peroxidase (CWPO-C) regulates the plant growth and affects auxin concentration in Arabidopsis thaliana

Yoshikay-Benitez

Yokoyama

Ohira

et al. 2022

Physiol Mol Biol Plants

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The poplar cationic cell-wall-bound peroxidase (CWPO-C) mediates the oxidative polymerization of lignin precursors, especially sinapyl alcohols, and high molecular weight compounds that cannot be oxidized by other plant peroxidases, including horseradish peroxidase C. Therefore, CWPO-C is believed to be a lignification-specific peroxidase, but direct evidence of its function is lacking. Thus, the CWPO-C expression pattern in Arabidopsis thaliana (Arabidopsis) was determined using the β-glucuronidase gene as a reporter. Our data indicated that CWPO-C was expressed in young organs, including the meristem, leaf, root, flower, and young xylem in the upper part of the stem. Compared with the wild-type control, transgenic Arabidopsis plants overexpressing CWPO-C had shorter stems. Approximately 60% of the plants in the transgenic line with the highest CWPO-C content had curled stems. These results indicate that CWPO-C plays a role in cell elongation. When plants were placed horizontally, induced CWPO-C expression was detected in the curved part of the stem during the gravitropic response. The stem curvature associated with gravitropism is controlled by auxin localization. The time needed for Arabidopsis plants overexpressing CWPO-C placed horizontally to bend by 90° was almost double the time required for the similarly treated wild-type controls. Moreover, the auxin content was significantly lower in the CWPO-C-overexpressing plants than in the wild-type plants. These results strongly suggest that CWPO-C has pleiotropic effects on plant growth and indole-3-acetic acid (IAA) accumulation. These effects may be mediated by altered IAA concentration due to oxidation.

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An improved transformation system for Lombardy poplar (Populus nigravar.italica)

Cited by 10 publications

References 22 publications

An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Transformation as a Tool for Genetic Analysis in Populus

Populus alba cationic cell-wall-bound peroxidase (CWPO-C) regulates the plant growth and affects auxin concentration in Arabidopsis thaliana

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