An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose Part I. Qualitative separation

Haworth, C.; Heathcote, J. G.

doi:10.1016/0021-9673(64)80151-5

Cited by 76 publications

(10 citation statements)

References 5 publications

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“…The chromatograms were developed with 2-propanol-methyl ethyl ketone-1 N HCI (60:15:25). Plates were allowed to develop to 13 cm (about 2.5 h) by the method of Haworth and Heathcote (10). The plates were dried under infrared heat for about 5 min, and the chromatograms were sprayed with a ninhydrin reagent (Gelman Instrument Co., Ann Arbor, Mich.) and placed in a drying oven.…”

Section: Methodsmentioning

confidence: 99%

Basis for the Design of Anticandidal Agents from Studies of Peptide Utilization in Candida albicans

Lichliter¹,

Naider²,

Becker

1976

Antimicrob Agents Chemother

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The growth of Candida albicans WD 18-4, a methionine and lysine double auxotroph, on a variety of methionine- and lysine-containing peptides was determined. This yeast does not excrete extracellular peptidases. Thus, the growth response to peptides containing the required amino acid is a measure of peptide transport. A variety of methionine-containing peptides such as Met-Met, Met-Met-Met, and Met-Met-Met-Met-Met are transported. Acylation of the N-terminus of transported peptides does not affect their transport, but derivitization of the C-terminus prevents peptide uptake. In contrast, all lysine-containing peptides tested, except Lys-Gly, were not growth substrates. The inability of a peptide to substitute for the requisite amino acid was not due to the absence of cellular peptidases or to toxicity of the nonutilized peptides. Several potentially toxic amino acids were carried into Candida as a component of transported peptides. This establishes the peptide transport system as a possible tool for the design of antibiotics for Candida albicans .

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Section: Methodsmentioning

confidence: 99%

Basis for the Design of Anticandidal Agents from Studies of Peptide Utilization in Candida albicans

Lichliter¹,

Naider²,

Becker

1976

Antimicrob Agents Chemother

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“…Aliquots (50 ,ul) of the redissolved aqueous fraction were chromatographed two-dimensionally on cellulose thin layers as described by Haworth and Heathcote (10). The isobutyric acid-based solvent of Crowley et al (9) was used in the first dimension and n-butylalcohol-acetic acid-water (12:3:5, v/v/v) in the second.…”

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confidence: 99%

Effect of Water Stress on Proline Synthesis from Radioactive Precursors

et al. 1976

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Barley (ffordeum vulgare L. var. Prior) leaves converted more '4C-glutamic acid to free proline when water-stressed than when turgid; neither decreased protein synthesis nor isotope trapping by the enlarged free proline pools found in wilted tissue seemed to account for the result. This apparent stimulation of proline biosynthesis in wilted leaves was not observed when radioactive ornithine or P5C (A1-pyrroline-5-carboxylate, an intermediate following glutamate in proline synthesis) were used as proline precursors unless proline levels were high as a result of previous water stress. We interpret this to mean that any stimulation of proline synthesis by water stress must act on P5C formation rather than its reduction to proline. Experiments showing greater apparent conversion of 14C-glutamate to proline do not unequivocally prove that proline synthesis is stimulated by water stress, as P5C feeding studies show that proline oxidation is inhibited under comparable conditions. This inhibition could account, at least in part, for increased proline labeling, and must be considered an alternate possibility.Although several plants have been reported to accumulate free proline during periods of water deficit (2,12,15,16,18) or when subjected to other stress (7, 8), the biochemical changes linking water stress and proline accumulation are not well understood. Barnett and Naylor (2) and Morris et al. (13) have observed increased incorporation of 14C-glutamic acid into proline in wilted leaves as compared to turgid leaves of Bermuda grass and turnip. This increase in radiotracer incorporation might reflect a stimulation of proline biosynthesis by water stress, but the data presented did not fully eliminate the possibility that inhibited protein synthesis might account for the results.The Bermuda grass experiment was done after a long period of drought, so that the large amount of free proline already present during "4C-glutamate feeding could have acted as a trapping pool for radioactive proline, accounting for the results in the absence of an actual increase in the rate of conversion of glutamate to proline. In this paper we describe 14C-glutamate feeding experiments designed to avoid these difficulties in interpretation, and to localize the point in the proline biosynthetic pathway at which a stress-induced stimulation would most likely occur.

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“…Salient features of the changes in glucose metabolism during the early hours of germination are as follows. (a) Glucose uptake increases 2-fold by 3 hr and 4-fold by 7 hr. (b) The utilization of labeled glucose in the tissue increases from 54% at I hr to 85% at 3 hr, and remains at that level by 7 hr.…”

Section: Resultsmentioning

confidence: 99%

“…Tritiated alanine and glutamic acid were used as internal markers in some of the separations. The components of each fraction were then identified by TLC according to Haworth and Heathcote (7). The organic acid fraction was applied to an AG 1-formate form column (1 x 14 cm), and eluted with 400 ml of a linear gradient of 0 to 6 N formic acid at a rate of 0.9 ml/min.…”

Section: Methodsmentioning

confidence: 99%

Glucose Metabolism and Retention of Glucose Metabolites in the Wheat Embryo during Early Germination

Ginzburg¹

1978

Plant Physiol.

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There is a lag period in the growth of excised wheat (Tritkum vulgare Host.) embryos extending from 0.5 hour to 4.5 hours of their imbibition in water. During this time there is a sharp increase in the embryos' capability to retain several amino and organic acids, to synthesize cell wall components and starch, and to take up glucose. Their capability to metabolize glucose also increases by 30%. Elevation of the ATP content of 1-hour embryos by incubation in 3 mM adenosine is not sufficient to bring about these changes. These changes may be a part of a metabolic adjustment in the embryos which increases their growth potential.

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An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose Part I. Qualitative separation

Cited by 76 publications

References 5 publications

Basis for the Design of Anticandidal Agents from Studies of Peptide Utilization in Candida albicans

Basis for the Design of Anticandidal Agents from Studies of Peptide Utilization in Candida albicans

Effect of Water Stress on Proline Synthesis from Radioactive Precursors

Glucose Metabolism and Retention of Glucose Metabolites in the Wheat Embryo during Early Germination

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