: An improved technique for mounting Heterodera cysts for light microscopy

Correia, Fernando; Abrantes, Isabel

doi:10.1163/005125997x00101

Cited by 3 publications

(2 citation statements)

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“…Stylet knob shape of the second-stage juveniles J2 was also used for PCNs identification ( EPPO, 2017 PM 7/40). The vulval cones are mounted in a 2% glycerin-agar medium as described by Correia and Abrantes (1997) and the collected larvae are killed under a low flame and then transferred to a drop of immersion oil or a drop of glycerol. Observations and measurements were made using an Olympus BX43 microscope.…”

Section: Methodsmentioning

confidence: 99%

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

Hajjaji

Mhand

Rhallabi

et al. 2021

Journal of Nematology

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Potato cyst nematodes (PCNs) are the most important potato pest causing major crop losses across the world with a quarantine status in many countries. In Morocco, several potato crops are infected with PCNs and the monitoring of potato production as well as the control of import and export of potato seeds are currently carried out by morphological methods. The present work was aimed to use molecular and morphometric methods for identifying and differentiating PCN species in Morocco for the first time. The morphological identification of PCN species from collected soil samples were carried out using the shape of the cysts, the length of the stylet, the number of cuticular ridges, and the Granek’s ratio. The J2 had a slightly shorter body length, the number of cuticular ridges was 9 and the Granek’s ratio averaged 2.2. The morphobiometric analysis revealed proximity of the Moroccan population to G. pallida species. PCNs sampled from contaminated fields were analyzed molecularly using PCR. DNA amplification was performed using the multiplex PCR method and PCR-RFLP from the ITS region of the total genomic DNA compared to multiplex PCR-specific DNA sequences. All confirmed the presence G. pallida in all samples of the Moroccan PCN populations.

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Section: Methodsmentioning

confidence: 99%

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

Hajjaji

Mhand

Rhallabi

et al. 2021

Journal of Nematology

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“…Cyst and second stage juveniles were measured for identification (Mulvey and Golden, 1983). The glycerine–agar technique was used for vulval cone preparations of cyst (Correia and Abrantes, 1997). All cysts were identified as H. cruciferae .…”

Section: Extraction Of Cysts and Collecting Of Eggsmentioning

confidence: 99%

Biological Control of Heterodera cruciferae (Tylenchida: Heteroderidae) Franklin 1945 with Fluorescent Pseudomonas spp.

Aksoy¹,

Mennan²

2004

Journal of Phytopathology

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This study was conducted to access the ability of fluorescent Pseudomonas spp. to suppress cyst nematode Heterodera cruciferae, which was caused by reproduction. The yield loss of cruciferous plants in infested soil with H. cruciferae was examined in in vitro and in vivo experiments. For this purpose, fluorescent Pseudomonas spp. was isolated from infested soil with cyst nematode H. cruciferae and a selected strain of fluorescent Pseudomonas [Fluorescent Pseudomonas strain no. 6 (FPs6)] used on cyst, females and eggs of H. cruciferae to study the biological control. In the in vitro study, eggs of H. cruciferae were infected by FPs6 on King's B Agar and consequently the growth and hatching of eggs were inhibited. Furthermore, J1 and J2 being grown in diseased eggs as they were infected by FPs6. In the in vivo study, plant lengths, leaf areas, wet weights, dry weights and root lengths of seedlings were investigated. There were no considerable differences among FPs6, FsP6 + Cyst and Control groups on plant length and root length (P > 0.05). But there were considerable differences between Cyst and Control groups on plant length and root length (P > 0.05).

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Integrative diagnosis of carrot cyst nematode (Heterodera carotae) using morphology and several molecular markers for an accurate identification

et al. 2017

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: An improved technique for mounting Heterodera cysts for light microscopy

Cited by 3 publications

References 0 publications

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

Biological Control of Heterodera cruciferae (Tylenchida: Heteroderidae) Franklin 1945 with Fluorescent Pseudomonas spp.

Integrative diagnosis of carrot cyst nematode (Heterodera carotae) using morphology and several molecular markers for an accurate identification

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