An Improved System for in vitro Propagation of Banana (Musa acuminate L.) Cultivars

Farahani, Farah; Aminpoor, H.; Sheidai, M.; Noormohammadi, Zahra; Mazinani, Mehdi Hosseini

doi:10.3923/ajps.2008.116.118

Cited by 22 publications

(7 citation statements)

References 3 publications

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“…This implies that different combinations of hormone concentrations of Naphthalene acetic acid (NAA), 6-Benzylaminopurine (BAP) and Kinetin (KIN) could enhance direct multiple shoot induction and proliferation in maize genotypes. This conforms to the observations made by (Farahani et al, 2008;Krishna et al, 2013;Feyisola et al, 2015). The performance of shoot proliferation and rooting traits at different growth stages of in vitro regeneration of maize revealed that two, three and four weeks are not significantly different from each other for number of shoots, but significantly different (P<0.05) from first week.…”

Section: Pearson Correlation Coefficient Of In Vitro Treatment Combinations Of Auxin (Naa) Cytokinin (Bap and Kinetin) Growth Stages On Ssupporting

confidence: 93%

In vitro Regeneration and Proliferation of Maize (Zea mays L.) Genotypes through Direct Organogenesis

2019

JNSR

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The variability of in vitro regeneration and proliferation of maize genotypes POOLISSRQPMX, DTSR-WCO, TZLCOMP4C3, TZE COMP 3C2, EV99QPM, POP66 SR/ACR94-YQPM, DTSR-WC, SAMMAZ 19S-14DT and TZEE-YPOPSTRC4 was investigated. Explants were regenerated through direct organogenesis using 0.1 mg/l NAA + 2.0 mg/l BAP, 0.1 mg/l NAA + 2.0 mg/l KIN, 0.3 mg/l NAA + 3.0 mg/l BAP, 0.3 mg/l NAA + 3.0 mg/l KIN, 0.5 mg/l NAA + 4.0 mg/l BAP, 0.5 mg/l NAA + 4.0 mg/l KIN and MS medium only. The regenerated maize yielded the highest number of multiple shoots (1.70 cm) and shoot length (3.35 cm) on MS medium supplemented with 0.3 mg/l NAA + 3.0 mg/l BAP within 31 days. Genotype EV99QPM and DTSR-WCO had the highest number of shoots, rooting traits respectively.

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Section: Pearson Correlation Coefficient Of In Vitro Treatment Combinations Of Auxin (Naa) Cytokinin (Bap and Kinetin) Growth Stages On Ssupporting

confidence: 93%

In vitro Regeneration and Proliferation of Maize (Zea mays L.) Genotypes through Direct Organogenesis

2019

JNSR

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“…The use of thidiazuron in banana micropropagation could be more economical than adenine-based cytokinins [56]. But high concentrations of thidiazuron can induce appearance of abnormal forms [57]. Thidiazuron at 0.2 mg/l induces more abnormal forms than benzylaminopurine at 5 mg/l [58].…”

Section: Influence Of Growth Regulators On Banana and Plantain Micropropagationmentioning

confidence: 99%

Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

Agbadje

Agbidinoukoun

Zandjanakou-Tachin³

et al. 2021

EJBIO

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Bananas and plantains are among the most important food crops in Central and West Africa. Their plantation is lead to many problems. In the recent decades, biotechnology tools using in vitro culture technics are used for the mass and free disease plantlets production in order to increase the bananas production and the yield. The main way of in vitro tissue culture at this end is the direct organogenesis i.e., the ability of plant tissues to form various organs de novo by shoots or roots induction to differentiate from a cell or cell clusters. This review aims to summarize the main results obtained in the organogenesis of bananas and plantains (Musa spp.) under in vitro conditions and to identify the challenges during the process. The research articles used in this review show that micropropagation is a reliable alternative to conventional production system of bananas and plantains planting material. However, the use of the in vitro micropropagation for bananas and plantains entails choosing the optimal explant type and size according to objectives. Benzylaminopurine remains the preferred cytokinin for in vitro banana and plantain shoot proliferation, while the use of thidiazuron appears to be more and more common. Whichever cytokinin used, the optimal cytokinin concentration for shoot proliferation is genotype dependent. This review also focuses on the causes and control measures of the two major banana and plantain micropropagation constraints: lethal tissues browning/darkening and microbial contaminations. It showed that applying the suitable and available control measure, according to the evolution of culture, is necessary. All this available information on the in vitro conditions makes banana and plantain cultivars in vitro organogenesis possible.

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“…Hormones usually tend to show the maximum shooting response at its optimum concentration. Farahani et al (2008) reported that the shoot multiplication of Musa acuminata was affected by the concentration of BAP. Later in 2015, Ferdous et al revealed the maximum single shoot formation and longest shoot formation in M. acuminata at 0.5 mg/L BAP.…”

Section: Effect Of Cacl 2 On Shoot Necrosismentioning

confidence: 99%

Improved protocol for efficient regeneration of coconut (Cocos nucifera L.) anther derived embryos

Bandupriya

Waidyarathne²

2021

J. Natn. Sci. Foundation Sri Lanka

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CaCl 2 on the reduction of shoot necrosis was also tested. CaCl 2 showed a significant (p = 0.001) effect on reducing the shoot necrosis. The lowest occurrence of shoot necrosis (25.0 %) was observed in 4.0 mM CaCl 2 treatment. Continuous sub-culturing of shoots with initial signs of shoot necrosis to elevated CaCl 2 levels until the rooting stage facilitated the recovery. Transfer of the shoots frequently into a fresh medium was not beneficial for the suppression of necrosis. Shoots maintained in the medium enriched with 4.0 mM CaCl 2 were transferred for acclimatisation, and this is the first report of transferring haploid coconut plants to acclimatisation conditions. The rooted shoots produced through the optimised protocol were acclimatised successfully. The prevention of shoot loss due to shoot necrosis will be beneficial for further refinement of the coconut anther culture protocol.

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An Improved System for in vitro Propagation of Banana (Musa acuminate L.) Cultivars

Cited by 22 publications

References 3 publications

In vitro Regeneration and Proliferation of Maize (Zea mays L.) Genotypes through Direct Organogenesis

In vitro Regeneration and Proliferation of Maize (Zea mays L.) Genotypes through Direct Organogenesis

Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

Improved protocol for efficient regeneration of coconut (Cocos nucifera L.) anther derived embryos

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