Enterococcus faecalisis a Gram-positive commensal inhabitant of the intestinal tract of humans, animals, and insects. However, it is also an opportunistic pathogen and has emerged as a leading cause of hospital-acquired extraintestinal infections. Fluoroquinolones have been frequently used to treatE. faecalisinfections, and the emergence of fluoroquinolone-resistantE. faecalisstrains has recently been reported in several countries. Thus, the identifications of new antibiotics specifically directed toE. faecalismay be envisaged. In this paper, a new series ofN-1,3-benzothiazol-2-ylbenzamides have been designed, synthesized, and evaluated for theirin vitroantimicrobial activities. Among the tested compounds,3iwas active againstE. faecalis.