An Improved Screening Method for Evaluation of Resistance to Phytophthora Blight in Sesame Cultivars and Accessions

Oh, Eunyoung; Kim, Sung-Up; Asekova, Sovetgul; Yoon, Yisang; Lee, Jeong‐Dong

doi:10.9787/pbb.2018.6.3.305

Cited by 7 publications

(17 citation statements)

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“…Besides the RIL populations, the germplasm lines ( n = 87) were also evaluated for their pathogenic response to P. nicotianae . The infection range observed in the plants was categorized from 0–9 scale from P. nicotianae resistance to susceptible (Oh et al, 2018). Of the 87 lines, 21 lines were categorized as resistant (R), 4 lines as moderately resistant (MR), 12 lines as moderately susceptible (MS), and 50 lines as susceptible (S) to P. nicotianae isolate KACC48121.…”

Section: Resultsmentioning

confidence: 99%

“…Three P. nicotianae isolates (KACC48120, 48121, No2040) were selected from to test the seedling resistance of 87 sesame accessions, since they contained different virulence genes among each other and were the commonly used P. nicotianae isolates for resistance genetic analysis in Korea (Oh et al, 2018; Table S1). KACC48120, No2040, and KACC48121 were collected from Gyeongju (35°85’ N, 129°22’ E), Yecheon (36°65’ N, 128°45’ E) of Gyeongsangbuk-do provinces, and Naju, Jeollanam-do province (35°01’ N, 126°71’ E), respectively, in the sesame growing area of Jeollanam-do and Gyeongsangbuk-do provinces.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Considerable variability in reactions of sesame germplasm to P. nicotianae has been reported worldwide and several research groups have done germplasm evaluation studies to select for the resistant varieties (Oh et al, 2018). So far, the Korean National Agrobiodiversity Germplasm has identified four P. nicotianae isolates, namely ‘KACC48120’, ‘48121’, ‘No2526’ and ‘2040’ (Oh et al, 2018). Oh et al (2018) evaluated sesame genotypes for P. nicotianae disease resistance in greenhouse nursery that were artificially inoculated with four strains, namely, KACC48120, 48121, No2526 and 2040.…”

Section: Introductionmentioning

confidence: 99%

“…So far, the Korean National Agrobiodiversity Germplasm has identified four P. nicotianae isolates, namely ‘KACC48120’, ‘48121’, ‘No2526’ and ‘2040’ (Oh et al, 2018). Oh et al (2018) evaluated sesame genotypes for P. nicotianae disease resistance in greenhouse nursery that were artificially inoculated with four strains, namely, KACC48120, 48121, No2526 and 2040. These strains were collected from infected sesame plants in Gyeongju, Naju, Gunwi, and Yecheon province fields in South Korea.…”

Section: Introductionmentioning

confidence: 99%

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A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

Asekova

Kulkarni

et al. 2020

Preprint

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SummaryPhytophthora blight, caused by pathogen Phytophthora nicotianae, is responsible for a huge reduction in sesame (Sesamum indicum L.) crop yields. In this study, we utilized a combinatorial approach involving biparental QTL mapping and genome-wide association (GWAS) analysis to identify genes associated with Phytophthora blight resistance in sesame. Evaluation of resistant of the parental varieties (Goenbaek, Osan and Milsung) and the RILs of both the populations in greenhouse conditions suggested the qualitative nature of the trait.. The genetic map comprised thirteen LGs covering a total map length of 887.49 cM with an average inter-marker distance of 4.69 cM. Significant QTLs explaining phenotypic variation in the range of 2.25% to 69.24% were identified on chromosomes 10 and 13 (Chr10 and Chr13). A resistance locus detected on Chr10 was found to be highly significant. The association of this locus to PBR was also identified through BSA and single marker analysis in Goenbaek × Milsung cross and through genome-wide association mapping of 87 sesame accessions. The GWAS analysis identified 44 SNP loci significantly associated with Phytophthora disease-resistant traits on Chr10. Further, the haplotype block analysis conducted in order to find whether the SNPs associated with resistance in this study showed that the SNPs are in high LD with the resistance QTL. We obtained a total of 68 candidate genes, which included a number of defense-related R genes. One of the genes, SIN_1019016 (At1g58390) showed high expression in the resistant parent. The results from this study would be highly useful in identifying genetic and molecular factors associated with Phytophthora blight resistance in sesame.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

Asekova

Kulkarni

et al. 2020

Preprint

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An Integrated Approach of QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame (Sesamum indicum L.)

Asekova

Kulkarni

et al. 2021

Front. Plant Sci.

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Phytophthora blight (PB) caused by Phytophthora nicotianae is a highly destructive disease in sesame (Sesamum indicum L.). In this study, we used linkage mapping and genome-wide association study (GWAS) to identify quantitative trait loci (QTL) and candidate genes associated with PB resistance. The QTL mapping in 90 RILs of the Goenbaek × Osan cross using genotyping-by-sequencing detected significant QTLs for PB resistance on chromosome 10, explaining 12.79%–13.34% of phenotypic variation. Association of this locus to PB resistance was also revealed through bulked segregant analysis in second RIL population (Goenbaek × Milsung cross) comprising 188 RILs. The GWAS of 87 sesame accessions evaluated against three P. nicotianae isolates identified 29 SNPs on chromosome 10 significantly associated with PB resistance. These SNPs were located within a 0.79 Mb region, which co-located with the QTL intervals identified in RIL populations, and hence scanned for identifying candidate genes. This region contained several defense-related candidate R genes, five of which were selected for quantitative expression analysis. One of these genes, SIN_1019016 was found to show significantly higher expression in the resistant parent compared to that in the susceptible parents and selected RILs. Paired-end sequencing of the gene SIN_1019016 in parental cultivars revealed two synonymous SNPs between Goenbaek and Osan in exon 2 of coding DNA sequence. These results suggested SIN_1019016 as one of the candidate gene conferring PB resistance in sesame. The findings from this study will be useful in the marker-assisted selection as well as the functional analysis of PB resistance candidate gene(s) in sesame.

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Current Research Trends and Prospects for Yield and Quality Improvement in Sesame, an Important Oilseed Crop

et al. 2022

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Climate change is shifting agricultural production, which could impact the economic and cultural contexts of the oilseed industry, including sesame. Environmental threats (biotic and abiotic stresses) affect sesame production and thus yield (especially oil content). However, few studies have investigated the genetic enhancement, quality improvement, or the underlying mechanisms of stress tolerance in sesame. This study reveals the challenges faced by farmers/researchers growing sesame crops and the potential genetic and genomic resources for addressing the threats, including: (1) developing sesame varieties that tolerate phyllody, root rot disease, and waterlogging; (2) investigating beneficial agro-morphological traits, such as determinate growth, prostrate habit, and delayed response to seed shattering; (3) using wild relatives of sesame for wide hybridization; and (4) advancing existing strategies to maintain sesame production under changing climatic conditions. Future research programs need to add technologies and develop the best research strategies for economic and sustainable development.

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An Improved Screening Method for Evaluation of Resistance to Phytophthora Blight in Sesame Cultivars and Accessions

Cited by 7 publications

References 4 publications

A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

An Integrated Approach of QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame (Sesamum indicum L.)

Current Research Trends and Prospects for Yield and Quality Improvement in Sesame, an Important Oilseed Crop

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