An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus

Zhang, Lanlan; Luo, Qing; Qin, Fang; Wang, Yaping

doi:10.1016/j.jviromet.2010.06.009

Cited by 50 publications

(33 citation statements)

References 20 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nearly 97% of DEGs were expressed in all four tissues and only 3% of DEGs showed some kind of tissue specificity. This indicates that GCRV likely causes a multi-organ disease rather than a tissue-specific one, although obvious symptoms are only reported in the gills and intestines (Su et al, 2011(Su et al, , 2009Zhang et al, 2010).…”

Section: Analysis Of Tissue Specificity Of Gene Expressionmentioning

confidence: 99%

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

Shi

Huang

et al. 2014

Molecular Immunology

Self Cite

View full text Add to dashboard Cite

Section: Analysis Of Tissue Specificity Of Gene Expressionmentioning

confidence: 99%

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

Shi

Huang

et al. 2014

Molecular Immunology

Self Cite

View full text Add to dashboard Cite

“…However, grass carp reovirus (GCRV), a fatal pathogen to aquatic animals, can provoke severe hemorrhagic disease in fingerling and yearling populations of grass carp, and cause a mortality rate of up to 85% during an outbreak [1][2][3][4]. Due to the high virulence of GCRV, the production of grass carp is severely affected and large economic losses in freshwater aquaculture in China have occurred since the 1980s [5].…”

mentioning

confidence: 99%

Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

Chen

Sun

Liao

et al. 2013

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Grass carp (Ctenopharyngodon idella)is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse naïve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.

show abstract

“…RNA was extracted from SVCV-and GCRV-infected cells. DNA was extracted from CyHV-2-infected cells, and PCRs were carried out according to the references, Stone et al (2003) (SVCV), Zhang et al (2010) (GCRV), and Jeffery et al (2007) (CyHV2). The virus neutralization assay was used for the confirmation of IPNV-SP.…”

Section: Viral Susceptibilitymentioning

confidence: 99%

Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus

Jing

Gao

Zhang

et al. 2015

Fish Physiol Biochem

View full text Add to dashboard Cite

Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15-20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 10(6.8) and 10(5.9) TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future.

show abstract

An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus

Cited by 50 publications

References 20 publications

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus

Contact Info

Product

Resources

About