An improved rat brain-tumor model

Kobayashi, Naoki; Allen, Nicholas; Clendenon, Nancy R.; Ko, Li Wen

doi:10.3171/jns.1980.53.6.0808

Cited by 126 publications

(82 citation statements)

References 6 publications

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“…Before injection into the brain, 10 4 cells were suspended into 1 mL of DMEM with antibiotics (1%). The cell suspension was manually injected according to a method derived from (Kobayashi et al, 1980) using a 1 mL Hamilton syringe through a burr hole (0.8 mm diameter) in the right caudate nucleus (7.5 mm anterior to the zero ear-bars, 3.5 mm lateral to the midline, 3 mm depth from the dura). The syringe was gently removed 1 min after the injection.…”

Section: Tumor Implantationmentioning

confidence: 99%

From Homogeneous to Fractal Normal and Tumorous Microvascular Networks in the Brain

Risser

Plouraboué

Steyer

et al. 2006

J Cereb Blood Flow Metab

103

129

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We studied normal and tumorous three-dimensional (3D) microvascular networks in primate and rat brain. Tissues were prepared following a new preparation technique intended for high-resolution synchrotron tomography of microvascular networks. The resulting 3D images with a spatial resolution of less than the minimum capillary diameter permit a complete description of the entire vascular network for volumes as large as tens of cubic millimeters. The structural properties of the vascular networks were investigated by several multiscale methods such as fractal and powerspectrum analysis. These investigations gave a new coherent picture of normal and pathological complex vascular structures. They showed that normal cortical vascular networks have scaleinvariant fractal properties on a small scale from 1.4 lm up to 40 to 65 lm. Above this threshold, vascular networks can be considered as homogeneous. Tumor vascular networks show similar characteristics, but the validity range of the fractal regime extend to much larger spatial dimensions. These 3D results shed new light on previous two dimensional analyses giving for the first time a direct measurement of vascular modules associated with vessel-tissue surface exchange.

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Section: Tumor Implantationmentioning

confidence: 99%

From Homogeneous to Fractal Normal and Tumorous Microvascular Networks in the Brain

Risser

Plouraboué

Steyer

et al. 2006

J Cereb Blood Flow Metab

103

129

View full text Add to dashboard Cite

show abstract

“…The temperature dependence of the r 1 relaxivity was studied for the 0.965 mmol/L sample by recording an additional profile at 371C±11C. C6 rat glioma cells were unilaterally implanted into the right cerebral hemisphere (Kobayashi et al, 1980). Briefly, rats weighing 160 to 180 g were anesthetized (400 mg/kg chloral hydrate, intraperitoneally) and placed in a stereotaxic head holder.…”

Section: High-field Relaxationmentioning

confidence: 99%

Cerebral Blood Volume Quantification in a C6 Tumor Model Using Gadolinium per (3,6-Anhydro) α-Cyclodextrin as a New Magnetic Resonance Imaging Preclinical Contrast Agent

Lahrech

Perles-Barbacaru

Aous

et al. 2008

J Cereb Blood Flow Metab

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In magnetic resonance imaging (MRI), cerebral blood volume (CBV) quantification is dependent on the MRI sequence and on the properties of the contrast agents (CAs). By using the rapid steadystate T 1 method, we show the potential of gadolinium per (3,6-anhydro) a-cyclodextrin (Gd-ACX), a new MRI paramagnetic CA (inclusion complex of Gd 3 + with per (3,6-anhydro)-a-cyclodextrin), for the CBV quantification in the presence of blood-brain barrier lesions. After biocompatibility and relaxivity experiments, in vivo experiments on rats were performed on a C6 tumor model with 0.05 mmol Gd-ACX/kg ( < 1/10 of the median lethal dose) injected at a 25 mmol/L concentration, inducing neither nephrotoxicity nor hemolysis. On T 1 -weighted images, a signal enhancement of 170% appeared in vessels after injection, but not in the tumor (during the 1 h of observation), in contrast to the 90% signal enhancement obtained with Gd-DOTA (a clinical MRI CA) injected at a T 1 isoefficient dose. This result shows the absence of Gd-ACX extravasation into the tumor tissue and its confinement to the vascular space. Fractional CBV values were found similar to Gd-ACX and Gd-DOTA in healthy brain tissue and in the contralateral hemisphere of tumor-bearing rats, whereas only Gd-ACX was appropriate for CBV quantification in tumor regions.

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“…Its morphology and growth characteristics have been described in detail. [22][23][24] The F98 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (Gibco-BRL, Gaithersberg, MD, USA) supplemented with 10% fetal bovine serum, 2 mmol/L L-glutamine, 0.1 mM non-essential amino acids, 10 units/ml penicillin, and 10 g/ml streptomycin (Gibco-BRL). Transduced F98 glioma cells were maintained in media containing 350 g/ml Geneticin (G418) (Gibco-BRL).…”

Section: Cell Culturementioning

confidence: 99%

In vivo imaging and radioiodine therapy following sodium iodide symporter gene transfer in animal model of intracerebral gliomas

et al. 2002

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An improved rat brain-tumor model

Cited by 126 publications

References 6 publications

From Homogeneous to Fractal Normal and Tumorous Microvascular Networks in the Brain

From Homogeneous to Fractal Normal and Tumorous Microvascular Networks in the Brain

Cerebral Blood Volume Quantification in a C6 Tumor Model Using Gadolinium per (3,6-Anhydro) α-Cyclodextrin as a New Magnetic Resonance Imaging Preclinical Contrast Agent

In vivo imaging and radioiodine therapy following sodium iodide symporter gene transfer in animal model of intracerebral gliomas

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