An improved protocol for dilution of cryoprotectants from vitrified human blastocysts

Cho, Hyon-Jin; Son, Weon‐Young; Yoon, San-Hyun; Lee, Sung‐Hee; Lim, Jin‐Ho

doi:10.1093/humrep/17.9.2419

Cited by 94 publications

(52 citation statements)

References 16 publications

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“…cryodamage [26][27][28]. The volume of the blastocoel prior to vitrification negatively affects the outcome measures, [29] specifically re-expansion, cell proliferation, and DNA integrity [30].…”

Section: Introductionmentioning

confidence: 99%

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Darwish¹,

Magdi²

2016

J Assist Reprod Genet

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Purpose Blastocysts contain a large amount of fluid in the blastocoel, which may pose a risk for ice crystal formation during vitrification. This study aimed to evaluate the effectiveness of laser-induced artificial shrinkage of blastocoel before vitrification on clinical outcome. Methods Patients were divided into two groups: a control group with untreated, expanded blastocysts (n = 115) and a study group with blastocoel artificially eliminated by a laser pulse prior to vitrification (n = 309). Blastocyst survival, clinical pregnancy, and implantation rates were compared. Result(s) The survival rate was significantly higher in the study group compared with the control group (97.3 and 74.9 %, respectively; p > 0.01). The clinical pregnancy and implantation rates of the study group were significantly higher (p < 0.01) than that of the control group (clinical pregnancy, 67.2 vs. 41.1 %; implantation, 39.1 vs. 24.5 %. Conclusion(s) This study demonstrated that the removal of blastocoel fluid before vitrification by laser pulse of in vitroproduced human blastocysts significantly improves blastocyst survival, clinical pregnancy, and implantation rates.

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Section: Introductionmentioning

confidence: 99%

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Darwish¹,

Magdi²

2016

J Assist Reprod Genet

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“…Thereafter, the minimum-volume cooling (MVC) vitrification procedure characterized by extremely high cooling rates was found to be more effective in improving the viability of post-warm blastocysts. Several cryodevices, such as Cryotop [7], microgrid [8], hemi-straw [9], Cryoloop [10], and Cryotip [11], have been used to accelerate the cooling rate for cryopreservation of human blastocysts. It is unlikely that the ovarian-uterine conditions during stimulated cycle equally support the implantation of transferred blastocysts when compared with those in non-stimulated cycles.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

Iwayama¹,

Hochi

Yamashita³

2010

J Assist Reprod Genet

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Purpose This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts. Methods Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts. Results Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate. Conclusion Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates.

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“…The re-expansion grade after warming has been used to indicate blastocyst survivability and quality after vitrification [21][22][23][24][25][26] and the clinical pregnancy rate of rapidly re-expanding blastocysts (within 2-4 h) was more than double the rate of slowly re-expanding blastocysts [21]. In this study, blastocyst degeneration was not observed after TE biopsy and only euploid blastocysts were selected for embryo transfer.…”

Section: Discussionmentioning

confidence: 94%

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

Lee

2016

Fertility and Sterility

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An improved protocol for dilution of cryoprotectants from vitrified human blastocysts

Abstract: The present results indicated that a 6-step thawing method was more effective than a 2-step thawing method for thawing vitrified human blastocysts.

Cited by 94 publications

References 16 publications

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

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