An Improved Process for the Preparation of Glucose Oxidase Membrane Immobilized by Plasma-Polymerized Film

Response Characteristics of a Glucose Electrode with a Sensing Membrane Prepared by Plasma Polymerization

“…The membrane disk was removed from the electrode when daily work was finished, and the material was again soaked in the buffer solution. Composition and manipulation of the glucose electrode were the same as previously reported (11,12,16).…”

“…The PPPA coating on the substrate membranes was carried out with the same procedure as described in a previous paper (11,12). After preliminary evacuation of the plasma chamber to approximately 0.02 Torr, PA vapor was introduced into the plasma chamber to hold the inner pressure at about 0.1 Torr.…”

“…The immobilized GOD membrane was mounted over an amperometric oxygen electrode to form a glucose electrode that was proportionally responsive to the glucose concentrations of the sample solutions (11). Extensive study has also revealed that the highest intensity of the electrode response within the shortest time was obtained when the MP filter was chosen with a pore size of 2200 Å and the PPPA was deposited with a thickness of 22,000 Å onto the glossy side and 15,000 Å onto the rough side (12). The electrode worked the best for sample solutions at approximately pH 7.0 (12).…”

“…A new technology for plasma polymerization which immobilizes enzyme materials has been also introduced, and we have reported one successful application to the preparation of an immobilized glucose oxidase (GOD) membrane usable for composing a glucose-sensing electrode (11,12). The GOD enzyme was deposited within a porous substrate such as a Millipore (MP) filter, and the substrate was coated with a semipermeable thin film on both sides by the plasma polymerization of propargyl alcohol (2-propyn-1-ol (PA)).…”

“…Extensive study has also revealed that the highest intensity of the electrode response within the shortest time was obtained when the MP filter was chosen with a pore size of 2200 Å and the PPPA was deposited with a thickness of 22,000 Å onto the glossy side and 15,000 Å onto the rough side (12). The electrode worked the best for sample solutions at approximately pH 7.0 (12). It has been found that some behavior of the proposed electrode differs from that of other immobilized enzyme electrodes when the intensity of the electrode response during consecutive measurements, the long-term stability of the enzyme activity, and the effect of interfering components were studied.…”