An Improved Procedure for the Preparation of Human Serum Albumin from Placental Extracts

The Purification of Human Placental Plasminogen (Profibrinolysin)

“…Human placental fractions were obtained from normal human placentas and intrapartum blood by the cold ethanol techniques [9,10] developed in this laboratory for the routine pre paration of serum albumin and immunoglobulin G.…”

“…The concentration of heme was determined by using the absorption at 403 va.fi on a Beckman DU spectrophotometer cor rected for light-scattering. Thus, for a 1% protein solution, mg heme/ ml = (A403 0.01) X 0.008 where A403 m^and 0.01 was the light scattering correction for a 1% protein solution [9].…”

“…Freezing Precipitate II at -20°C caused the formation of a relatively Ppt. II [9,10] insoluble complex between plasminogen and other proteins and thus made assay and purification difficult.…”

The Fractionation of Heparinized Blood

“…(a) method 6 of Cohn, et al [4] ; (b) method 9 of Oncley, et al [12], and (c) zinc-ethanol procedures developed in this laboratory for frac tionation of placental extracts [7,17,18].…”

“…In a further experiment, a pool of heparinized plasma was processed using the zinc-ethanol system [7,17,18] developed and currently in use at the Michigan Department of Health for the preparation of both gamma globulin and albumin from placental extracts and intrapartum blood. By this method, the gamma globulin and albumin were both recovered in highly purified form (98.4 and 99 %, respectively, by electrophoretic analysis).…”