Polystyrene-based granulated support Bio-Beads® SM-2 was employed for the separation of peptide-pnitroanilides from Na 2 SO 4 and Na 2 SO 5 mixture (Oxone®) after oxidation of peptide-p-aminoanilides.Unsubstituted phenyl-containing N-terminal protection groups (such as carbobenzoxy or tested earlier benzoyl) and Phe residues ensured an e cient adsorption of peptide-p-nitroanilides onto Bio-Beads® SM-2. Neither such aromatic groups as tosyl, 9-uorenyl(methoxycarbonyl), p-nitroanilide nor indolyl or phydroxyphenyl groups of Trp and Tyr residues provide peptide-p-nitroanilides with the ability to adsorb onto the polystyrene-based support. Hence, peptides and their derivatives should contain at least one unsubstituted phenyl group to ensure an e cient separation and puri cation via adsorption chromatography on Bio-Beads® SM-2. This chromatographic support is well suitable for desalting or separation of phenyl group-containing peptides, especially in multiple parallel preparation and highthroughput screening experiments.