An improved preparation of ribosomes and polysomes from cardiac muscle

Requirement for Extraction of Polyribosomes from Barley Tissue

“…In contrast, increasing the pH and KCI above levels previously used for polyribosome extraction from barley tissue (27) has reduced the rate of conversion of polyribosomes to monosomes. High ionic strength extraction of ribosomes does not appear to be detrimental to in vitro protein synthesis (2,10,12,20). These modifications in extraction procedure have been useful in removing RNase from the ribosomes and actually inhibiting RNA degrading activity (14).…”

“…Stabilization of assembled ribosomes and retention of their protein synthesis capability has been accomplished in various animal tissues by increase of the ionic strength of the extraction medium (9,10,12,22). The primary explanation given to the ionic strength effect was one of dissociating basic proteins from the ribosomes (10,22).…”

“…The primary explanation given to the ionic strength effect was one of dissociating basic proteins from the ribosomes (10,22). Specifically, this step was considered important in the removal of RNase from ribosomal suspensions.…”

Polysomes of the Sea Urchin Embryo: An Improved Method for Extraction of Integrated Polysomes

“…Recently, it has been reported that EDTA at low concentrations (0.5 -1.0 mM), like bentonite, polyvinylsulfate and rat liver RNase inhibitor, is a potent inhibitor of a particular type of RNase (EARL and MORGAN, 1968;LAGA, BALIGA and MU", 1970;EKER and PIHL, 1971). Use of the reagent, EDTA-2K, was reported to increase the integrity of polysomes (EARL and MORGAN, 1968;LAGA, BALIGA and MUNN, 1970).…”

“…Use of the reagent, EDTA-2K, was reported to increase the integrity of polysomes (EARL and MORGAN, 1968;LAGA, BALIGA and MUNN, 1970).…”