An improved PCR-based method for faster sex determination in brown trout (Salmo trutta) and Atlantic salmon (Salmo salar)

Quéméré, Erwan; Perrier, Charles; Besnard, Anne‐Laure; Evanno, Guillaume; Baglinière, Jean-Luc; Guiguen, Yann; Launey, Sophie

doi:10.1007/s12686-014-0259-8

Cited by 39 publications

(53 citation statements)

References 6 publications

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“…Circles represent haplotypes, and their sizes are proportional to the number of individuals showing the haplotype. Three of the haplotypes described by Quéméré et al () were found: Haplotypes B and E in Spain (red), and haplotype D in all of the rivers studied. Haplotypes A and C (Quéméré et al, ) were not present in the samples.…”

Section: Resultsmentioning

confidence: 62%

“…Three of the haplotypes described by Quéméré et al () were found: Haplotypes B and E in Spain (red), and haplotype D in all of the rivers studied. Haplotypes A and C (Quéméré et al, ) were not present in the samples. Two haplotypes were described: Haplotype H in Spain and Scotland, and haplotye I in France.…”

Section: Resultsmentioning

confidence: 62%

“…Alignment of the haplotypes for the sdY partial intron sequence, including those described by Quéméré et al (): Haplotypes A and C were not found, haplotype B was found in the Masma River (Spain), haplotype D was found in all of the rivers sampled (Spain, France, Scotland, and Ireland), and haplotype E was found in the Pas River (Spain). Two novel haplotypes were found: Haplotype H in Spain and Scotland (in the rivers Bidasoa and Dee), and haplotype I in France (Nivelle River)…”

Section: Resultsmentioning

confidence: 83%

“…To evaluate the first intron of sdY , a short polymorphic fragment (~200 bp) of genomic DNA extracted from all male salmon, as a marker for measuring male‐mediated introgression, was amplified using primers Salmo‐ sdY ‐F and Salmo‐ sdY ‐R in the conditions described by Quéméré et al (). Amplification products were purified with ExoSAP‐IT® PCR Product Cleanup.…”

Section: Methodsmentioning

confidence: 99%

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A genetic tool for evaluating male‐mediated stock introgression in Atlantic salmon

Gabián

Morán

2019

Aquatic Conservation

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The Atlantic salmon shows strong population structure with reduced gene flow, owing to homing behaviour. Supportive breeding with allochtonous parr or fish‐farm escapees could affect native population genetic structure by the introgression of foreign genes. Mature male parr are potentially powerful vehicles for promoting introgression: they can fertilize eggs in competition with anadromous males. As a consequence, foreign males contribute more to introgression than foreign females. This effect must be taken into account for the correct development of conservation programmes. Foreign males constitute an inadvertent way to disrupt the genetic structure, as the allochtonous parr or fish‐farm escapees can survive in the river during the parr stage. A short polymorphic fragment of the sex‐determining gene (sdY) was used as a marker to assess introgression via males into south European salmon populations. In order to find haplotype variants for the intronic sequence of sdY, samples were investigated from 16 different rivers across the distribution range of the salmon, together with historical samples (1950–1960) from three Spanish rivers. Two novel haplotypes, in addition to the three already described in Atlantic salmon, were found for this locus. Most samples, including historical ones, displayed the common haplotype (D) previously found in salmon all over Europe; however, north European haplotype variants were also detected in salmon inhabiting the southern European rivers that were systematically supplemented with foreign stocks between 1981 and 1994. Analysis of haplotype variation in the sdY intron constitutes a powerful and inexpensive tool, not only to specifically assess male‐mediated introgression into natural populations of Atlantic salmon, but also for monitoring salmon escapees.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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A genetic tool for evaluating male‐mediated stock introgression in Atlantic salmon

Gabián

Morán

2019

Aquatic Conservation

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“…Yet, theories of sex allocation have rarely been tested in highly fecund fishes, due to the difficulty of sexing small embryos and the absence of sex-specific markers. The recent description of the master sex-determining gene sdY in rainbow trout [7] has made it possible for the first time to sex salmonid fishes at an early stage [8]. This presents an unprecedented opportunity in evolutionary ecology because skewed sex ratios are typical of many exploited fish populations [9], and these may vary widely from year to year [10].…”

Section: Introductionmentioning

confidence: 99%

The male handicap: male-biased mortality explains skewed sex ratios in brown trout embryos

2016

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Juvenile sex ratios are often assumed to be equal for many species with genetic sex determination, but this has rarely been tested in fish embryos due to their small size and absence of sex-specific markers. We artificially crossed three populations of brown trout and used a recently developed genetic marker for sexing the offspring of both pure and hybrid crosses. Sex ratios (SR = proportion of males) varied widely one month after hatching ranging from 0.15 to 0.90 (mean = 0.39 ± 0.03). Families with high survival tended to produce balanced or male-biased sex ratios, but SR was significantly female-biased when survival was low, suggesting that males sustain higher mortality during development. No difference in SR was found between pure and hybrid families, but the existence of sire × dam interactions suggests that genetic incompatibility may play a role in determining sex ratios. Our findings have implications for animal breeding and conservation because skewed sex ratios will tend to reduce effective population size and bias selection estimates.

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Development and Application of Sex‐Linked Markers in Salmonidae

Tolosa

Pastén

Iturra

2018

Sex Control in Aquaculture

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An improved PCR-based method for faster sex determination in brown trout (Salmo trutta) and Atlantic salmon (Salmo salar)

Cited by 39 publications

References 6 publications

A genetic tool for evaluating male‐mediated stock introgression in Atlantic salmon

A genetic tool for evaluating male‐mediated stock introgression in Atlantic salmon

The male handicap: male-biased mortality explains skewed sex ratios in brown trout embryos

Development and Application of Sex‐Linked Markers in Salmonidae

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