An improved orthotopic rat bladder tumor model using DiI-loaded fluorescent AY-27 cells

Vandepitte, Joachim; Maes, J.M.; Cleynenbreugel, Ben Van; Poppel, Hein Van; Lerut, Evelyne; Agostinis, Patrizia; Witte, Peter de

doi:10.4161/cbt.9.12.11638

Cited by 11 publications

(11 citation statements)

References 27 publications

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“…Culturing methods and the technique used for tumour implantation were described previously by Vandepitte et al. . Briefly, rats were anesthetised with an i.p.…”

Section: Methodsmentioning

confidence: 99%

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Biodistribution of Evans blue in an orthotopic AY‐27 rat bladder urothelial cell carcinoma model: implication for the improved diagnosis of non‐muscle‐invasive bladder cancer (NMIBC) using dye‐guided white‐light cystoscopy

et al. 2015

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EB is selectively taken up by tumour tissue after intravesical instillations in rats bearing bladder tumours. The lower expression of desmoglein-1 in tumour samples, together with the reduced presence of desmosomes seen with TEM, likely imply that desmosomes play an important role in the ultrastructural differences between healthy rat urothelium and tumour tissue, and secondary to that, to the differential uptake of EB in both tissues. We consider that our findings could be useful for future clinical developments in the field of diagnostics for bladder cancer.

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“…Culturing methods and the technique used for tumour implantation were described previously by Vandepitte et al. . Briefly, rats were anesthetised with an i.p.…”

Section: Methodsmentioning

confidence: 99%

“…To obtain an intact and superficial non‐muscle‐invasive bladder tumour, in our hands malignant cells needed to grow for 1–3 days after cell inoculation . Therefore, all experiments were performed 2 days after the inoculation of AY‐27 cells into the rat bladder.…”

Section: Methodsmentioning

confidence: 99%

Biodistribution of Evans blue in an orthotopic AY‐27 rat bladder urothelial cell carcinoma model: implication for the improved diagnosis of non‐muscle‐invasive bladder cancer (NMIBC) using dye‐guided white‐light cystoscopy

et al. 2015

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“…Different animal models of preclinical BCa have been developed. Most of these models consist of chemically induced BCa, implanting xenografts generated from well‐established human BCa cell lines that have adapted to in vitro growth into immunodeficient mice, or transplanting carcinogen‐induced BCa in syngeneic, immunocompetent mice . Although such models have been useful, they also have limitations.…”

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confidence: 99%

Development and characterization of a bladder cancer xenograft model using patient‐derived tumor tissue

et al. 2013

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Most of the cancer xenograft models are derived from tumor cell lines, but they do not sufficiently represent clinical cancer characteristics. Our objective was to develop xenograft models of bladder cancer derived from human tumor tissue and characterize them molecularly as well as histologically. A total of 65 bladder cancer tissues were transplanted to immunodeficient mice. Passagable six cases with clinico-pathologically heterogeneous bladder cancer were selected and their tumor tissues were collected (012T, 025T, 033T, 043T, 048T, and 052T). Xenografts were removed and processed for the following analyses: (i) histologic examination, (ii) short tandem repeat (STR) genotyping, (iii) mutational analysis, and (iv) array-based comparative genomic hybridization (array-CGH). The original tumor tissues (P 0) and xenografts of passage 2 or higher ( ! P2) were analyzed and compared. As a result, hematoxylin and eosin staining revealed the same histologic architecture and degree of differentiation in the primary and xenograft tumors in all six cases. Xenograft models 043T_P2 and 048T_P2 had completely identical STR profiles to the original samples for all STR loci. The other models had nearly identical STR profiles. On mutational analysis, four out of six xenografts had mutations identical to the original samples for TP53, HRAS, BRAF, and CTNNB1. Array-CGH analysis revealed that all six xenograft models had genomic alterations similar to the original tumor samples. In conclusion, our xenograft bladder cancer model derived from patient tumor tissue is expected to be useful for studying the heterogeneity of the tumor populations in bladder cancer and for evaluating new treatments. (Cancer Sci 2013; 104: 631-638)

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“…In the context of studying neuroinflammation, some of the earliest studies to visualize the immune response in situ were performed using adoptive transfer of exogenously labeled cells. Cell populations collected in vivo were purified and labeled with fluorescent dyes with non-overlapping emission spectra, such as CFSE [ 31 ], SNARF [ 32 ], CMAC [ 33 ] or DiI [ 34 ]. Although it has been useful for short-term trafficking and migration studies, cell division however resulted in the dilution of dyes and made them difficult to track.…”

Section: Fluorescent Labelsmentioning

confidence: 99%

Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents

Rougon

Brasselet

Debarbieux

2016

BPL

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Purpose of review: Highly coordinated cellular interactions occur in the healthy or pathologic adult rodent central nervous system (CNS). Until recently, technical challenges have restricted the analysis of these events to largely static modes of study such as immuno-fluorescence and electron microscopy on fixed tissues. The development of intravital imaging with subcellular resolution is required to probe the dynamics of these events in their natural context, the living brain.Recent findings: This review focuses on the recently developed live non-linear optical imaging modalities, the core principles involved, the identified technical challenges that limit their use and the scope of their applications. We highlight some practical applications for these modalities with a specific attention given to Experimental Autoimmune Encephalomyelitis (EAE), a rodent model of a chronic inflammatory disease of the CNS characterized by the formation of disseminated demyelinating lesions accompanied by axonal degeneration.Summary: We conclude that label-free nonlinear optical imaging combined to two photon imaging will continue to contribute richly to comprehend brain function and pathogenesis and to develop effective therapeutic strategies.

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An improved orthotopic rat bladder tumor model using DiI-loaded fluorescent AY-27 cells

Cited by 11 publications

References 27 publications

Biodistribution of Evans blue in an orthotopic AY‐27 rat bladder urothelial cell carcinoma model: implication for the improved diagnosis of non‐muscle‐invasive bladder cancer (NMIBC) using dye‐guided white‐light cystoscopy

Biodistribution of Evans blue in an orthotopic AY‐27 rat bladder urothelial cell carcinoma model: implication for the improved diagnosis of non‐muscle‐invasive bladder cancer (NMIBC) using dye‐guided white‐light cystoscopy

Development and characterization of a bladder cancer xenograft model using patient‐derived tumor tissue

Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents

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