An improved non-cryogenic transport and storage preservative facilitating DNA extraction from 'difficult' plants collected at remote sites

Thomson, John A.

doi:10.7751/telopea20024013

Cited by 29 publications

(37 citation statements)

References 13 publications

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“…Tissue was frozen in liquid nitrogen and stored at )70°C, dried using silica gel (Chase and Hills, 1991) or preserved in a CTAB/salt solution (Thomson, 2002). DNA was extracted using a modified CTAB protocol (Doyle and Doyle, 1990).…”

Section: Methodsmentioning

confidence: 99%

Using matK sequence data to unravel the phylogeny of Casuarinaceae

Steane

Wilson

Hill

2003

Molecular Phylogenetics and Evolution

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Casuarinaceae are a Gondwanic family with a unique combination of morphological characters not comparable to any other family. Until recently, the 96 species in the family were classified in a single genus, Casuarina s.l. A recent morphological revision of the family resulted in the splitting of Casuarina s.l. into four genera-Allocasuarina, Casuarina s.s., Ceuthostoma, and Gymnostoma. This study uses matK sequence data from 76 species of Casuarinaceae and eight outgroup taxa to examine the phylogenetic structure within the Casuarinaceae. The study demonstrates the monophyly of the four genera and examines the relationships within the family; it tests the validity of the infra-generic subdivision of Allocasuarina; it discovers geography-based infra-generic subdivisions within Gymnostoma and Casuarina; and, finally, provides a molecular framework on which to trace the evolution of xeromorphy in the Casuarinaceae. Crown

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Section: Methodsmentioning

confidence: 99%

Using matK sequence data to unravel the phylogeny of Casuarinaceae

Steane

Wilson

Hill

2003

Molecular Phylogenetics and Evolution

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“…Leaf material was dried with silica gel to rapidly desiccate the material and reduce DNA degradation before extraction (Chase & Hills 1991) or with the alternative preservative solution of saturated CTAB to reduce degradative changes affecting the quality of DNA (Thomson 2002). A few samples were taken from herbarium specimens and some DNA was available in the Botany Molecular Laboratory, Trinity College, Dublin and the Royal Botanic Gardens, Kew, England, U.K.…”

Section: Taxonomic Samplementioning

confidence: 99%

Phylogenetics of Panicoideae (Poaceae) based on chloroplast and nuclear DNA sequences

Teerawatananon¹,

Jacobs²,

Hodkinson³

2011

Telopea

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Phylogenetic relationships among major subfamilies in Poaceae and among major tribes within Panicoideae were evaluated using parsimony and Bayesian analyses of chloroplast trnL-F and atpβ-rbcL DNA sequences and a nuclear ribosomal DNA sequence, ITS1-ITS2. The PanicoideaeAristidoideae-Chloridoideae-Micrairoideae-Arundinoideae-Danthonioideae (PACMAD) clade was well resolved. A close relationship between Aristidoideae and Chloridoideae was found. The monophyly of Micrairoideae was resolved but the relationships of three tribes (Eriachneae, Isachneae, Micraireae) within Micrairoideae were unclear, only Eriachne and Isachne were monophyletic. Panicoideae sensu stricto were supported as monophyletic and sister to a clade of Danthoniopsis and Tristachya. Within Panicoideae, only a clade of Andropogoneae + Arundinella + Garnotia was supported. None of the analyses supported the monophyletic status of Paniceae. Within Paniceae, the bristle clade (excluding Cenchrus) + Alexfloydia, and the forest shade clade sensu Giussani et al. (2001), were found, but their circumscription remains ambiguous. A sister relationship between the endemic and rare Australian grasses Homopholis and Walwhalleya was also resolved. Arundinelleae were found to be polyphyletic. This study supported the separation of Arundinella and Garnotia from the remaining Arundinelleae and the inclusion of both genera in their own subtribes (Arundinellinae Honda sensu stricto and Garnotiinae Pilger) within the Andropogoneae. Arundinelleae should be abandoned as a taxonomic tribe within the Centothecoid + Panicoid clade. Within Andropogoneae, five out of a total of 11 subtribes (Chionachninae, Coicinae, Dimeriinae, Germainiinae, and Tripsacinae) were monophyletic. This was the first time that Dimeriinae and Germainiinae have been included in a molecular study. † Deceased November 26 2009. Telopea 13(1-2): 2011Teerawatananon, Jacobs and Hodkinson

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“…Material was silica dried (Chase and Hillis 1991) or conserved in CTAB (saturated brine containing cetyl trimethylammonium bromide/disinfectant; Rogstad 1992, modified by Thomson 2002) and mostly collected specifically for this project by Kerri Clarke, Karen Wilson, and Jeremy Bruhl as cited in Table 2.…”

Section: Plant Samplesmentioning

confidence: 99%

Phylogeny of Abildgaardieae (Cyperaceae) Inferred from ITS and trnL–F Data

Ghamkhar¹,

Marchant²,

Wilson³

et al. 2007

aliso

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Within the tribe Abildgaardieae, the relationships between Fimbristylis and its relatives have not been certain, and the limits of Fimbristylis have been unclear, with Bulbostylis and Abildgaardia variously combined with it and each other. The relationships and limits of tribes Abildgaardieae and Arthrostylideae and their genera were evaluated across 49 representative species using parsimony and maximum likelihood analyses of ITS (nuclear ribosomal) and trnL-F (plastid) DNA sequence data separately and combined. The evolutionary reconstructions derived from sequences of cpDNA and nrDNA disagree about the position of tribe Arthrostylideae relative to Abildgaardieae; Arthrostylis and Actinoschoenus are either nested within Abildgaardieae (trnL-F data) or very closely related to this tribe (ITS data). The reconstructions also disagree about the monophyly of genus Abildgaardia (excluding A. vaginata). Crosslandia and A. vaginata form a clade that is nested within Fimbristylis. Bulbostylis is monophyletic and clearly separated from Fimbristylis. Further sampling of taxa and characters is needed to resolve and/or strengthen support for some of these ''deep'' and fine-scale relationships.

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An improved non-cryogenic transport and storage preservative facilitating DNA extraction from 'difficult' plants collected at remote sites

Cited by 29 publications

References 13 publications

Using matK sequence data to unravel the phylogeny of Casuarinaceae

Using matK sequence data to unravel the phylogeny of Casuarinaceae

Phylogenetics of Panicoideae (Poaceae) based on chloroplast and nuclear DNA sequences

Phylogeny of Abildgaardieae (Cyperaceae) Inferred from ITS and trnL–F Data

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