An improved microculture method for diagnosis of cutaneous leishmaniasis

Pagheh, Abdol Sattar; Fakhar, Mahdi; Mesgarian, Fatemeh; Gholami, Shirzad; Ahmadpour, Ehsan

doi:10.1007/s12639-013-0316-3

Cited by 16 publications

(7 citation statements)

References 20 publications

(39 reference statements)

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“…Cutaneous leishmaniasis individuals were considered for enrolment if they presented with active skin lesions of recent onset of infection. Samples were obtained by lesion scrapping using a vaccinostyle, under sterile conditions; moreover, the laboratory confirmation of CL patients was done through direct microscopy on Giemsa staining lesion smears, lesion aspirate microculture and PCR‐RFLP/nested PCR . The personal information of each CL confirmed patient was recorded (sex, age, lesion duration, type and number, and drug consumption) (Table ).…”

Section: Methodsmentioning

confidence: 99%

Arginase activity of Leishmania isolated from patients with cutaneous leishmaniasis

Badirzadeh

Taheri

Abedi-Astaneh

et al. 2017

Parasite Immunology

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Cutaneous leishmaniasis (CL) is one of the most important vector-borne parasitic diseases, highly endemic in Iran, and its prevalence is increasing all over the country. Arginase (ARG) activity in isolated Leishmania parasites from CL patients is yet to be explored. This study aimed to compare the ARG activity of isolated Leishmania promastigotes from CL patients with a standard strain of Leishmania major and its influences on the disease pathogenesis. We recruited 16 confirmed CL patients from Qom Province, in central Iran; after detection of Leishmania species using PCR-RFLP, we assessed the levels of ARG in the isolated promastigotes and determined the parasites' growth rate. Only L. major was identified from CL patients. The level of ARG activity in the isolated Leishmania promastigotes from CL patients was significantly higher than that obtained from the standard strain of L. major. No significant correlations between ARG activity and lesion size, number or duration were observed; in contrast, a significant negative correlation was seen between ARG level and Leishmania' growth rate. The obtained results suggest that increased ARG expression and activity in the isolated Leishmania promastigotes might contribute to the higher parasite infectivity and play a major role in the pathogenicity of the CL.

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Section: Methodsmentioning

confidence: 99%

Arginase activity of Leishmania isolated from patients with cutaneous leishmaniasis

Badirzadeh

Taheri

Abedi-Astaneh

et al. 2017

Parasite Immunology

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“…In a recent study, the performance of micro-culture was assessed on 273 subjects who fulfilled the criteria for CL. Sensitivity and specificity for micro-culture were 98.4 % (95 % CI = 96.1–99.1 %) and 100.0 %, respectively [ 14 ]. Additional advantages of this method are simplicity and the fact that diagnostic samples are retrieved by a needle-free method.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Cutaneous Leishmaniasis: Recent Developments in Diagnosis and Management

2015

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This review focuses on recent developments in the diagnosis, treatment, management, and strategies for the prevention and control of cutaneous leishmaniasis (CL) caused by both Old and New World Leishmania species. CL is caused by the vector-borne protozoan parasite Leishmania and is transmitted via infected female sandflies. The disease is endemic in more than 98 countries and an estimated 350 million people are at risk. The overall prevalence is 12 million cases and the annual incidence is 2–2.5 million. The World Health Organization considers CL a severely neglected disease and a category 1 emerging and uncontrolled disease. The management of CL differs from region to region and is primarily based on local experience-based evidence. Most CL patients can be treated with topical treatments, but some Leishmania species can cause mucocutaneous involvement requiring a systemic therapeutic approach. Moreover, Leishmania species can vary in their sensitivity to available therapeutic options. This makes species determination critical for the choice of treatment and the clinical outcome of CL. Identification of the infecting parasite used to be laborious, but now the Leishmania species can be identified relatively easy with new DNA techniques that enable a more rational therapy choice. Current treatment guidelines for CL are based on poorly designed and reported trials. There is a lack of evidence for potentially beneficial treatments, a desperate need for large well-conducted studies, and standardization of future trials. Moreover, intensified research programs to improve vector control, diagnostics, and the therapeutic arsenal to contain further incidence and morbidity are needed.

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“…A drawback of micro-culture is that this technique does not allow for further species determination [27].…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological presentation of cutaneous leishmaniasis and its diagnostic algorithm

Saini¹,

A²

2017

APJTD

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An improved microculture method for diagnosis of cutaneous leishmaniasis

Cited by 16 publications

References 20 publications

Arginase activity of Leishmania isolated from patients with cutaneous leishmaniasis

Arginase activity of Leishmania isolated from patients with cutaneous leishmaniasis

Cutaneous Leishmaniasis: Recent Developments in Diagnosis and Management

Clinicopathological presentation of cutaneous leishmaniasis and its diagnostic algorithm

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