An improved method to obtain novel mutants in Cucurbita pepo by pollen viability

Vicente-Dólera, Nelly; Pinillos, V.; Moya, M.; Río-Celestino, Mercedes Del; Pomares-Viciana, Teresa; Román, Belén; Gómez, Pedro Sánchez

doi:10.1016/j.scienta.2014.01.045

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The development of a method for selecting M1 lines with higher fertility used in the first steps of the generation of this current platform [20] was successful. Despite the mutagen's toxicity, the analysis of M2 and M3 lines performed in this study demonstrate that this TILLING platform consists of fertile lines with mutations affecting most phenological stages of development.…”

Section: Discussionmentioning

confidence: 99%

“…The TILLING platform validated here is the first developed in Cucurbita pepo , probably because the low fertility that the mutants of this species have shown in previous attempts, even using different varieties [32] , has hampered the perpetuation of the populations. The development of a method for selecting M1 lines with higher fertility used in the first steps of the generation of this current platform [20] was successful. Despite the mutagen's toxicity, the analysis of M2 and M3 lines performed in this study demonstrate that this TILLING platform consists of fertile lines with mutations affecting most phenological stages of development.…”

Section: Discussionmentioning

confidence: 99%

“…Four to nine seeds of each of the 1464 M2 families from a mutant population previously generated with three different EMS doses: 80 mM, 65 mM and 40 mM [20] were sown and grown in greenhouses following standard local commercial practices for both plant nutrition and pest and disease control. Germination and sterility rates of the M2 families were evaluated and controlled self-pollinations were attempted on each plant.…”

Section: Methodsmentioning

confidence: 99%

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First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

Vicente-Dólera

Troadec²,

Moya

et al. 2014

PLoS ONE

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Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

Vicente-Dólera

Troadec²,

Moya

et al. 2014

PLoS ONE

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“…Cavili F 1 produces light green skinned fruits and Parador F 1 produced golden yellow skinned fruits, with differences in flavor and textures between both kinds of fruits. Secondly, lines derived from a mutant population previously generated with three different ethyl methanesulfonate doses . This material represents a wide diversity available to zucchini breeding programmes.…”

Section: Methodsmentioning

confidence: 99%

Characterisation and prediction of carbohydrate content in zucchini fruit using near infrared spectroscopy

et al. 2017

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The coefficients of determination in the external validation (R VAL) ranged from 0.66 to 0.85. The standard deviation (SD) to standard error of prediction ratio (RPD) and SD to range (RER) were variable for different quality compounds and showed values that were characteristic of equations suitable for screening purposes. From the study of the MPLS loadings of the first three terms of the different equations for sugars and starch, it can be concluded that some major cell components such as pigments, cellulose, organic acids highly participated in modelling the equations for carbohydrates. © 2017 Society of Chemical Industry.

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Biomarkers for selection of parthenocarpic zucchini cultivars

Pomares-Viciana¹,

Rio²,

Cabrera³

et al. 2017

Acta Hortic.

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An improved method to obtain novel mutants in Cucurbita pepo by pollen viability

Cited by 4 publications

References 23 publications

First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

Characterisation and prediction of carbohydrate content in zucchini fruit using near infrared spectroscopy

Biomarkers for selection of parthenocarpic zucchini cultivars

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