An improved method of mouse liver micronucleus analysis: an application to age-related genetic alteration and polyploidy study

Uryvaeva, I. V.; Delone, G. V.

doi:10.1016/0165-1161(95)90032-2

Cited by 26 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A positive UDS result was seen in a similar study by Ikegwuonu and Mehendale [1991] but in a follow-up investigation reported in the same article, they concluded that the increases were due to technical problems with the use of hydroxyurea in the assay. In tests measuring chromosomal alterations, CT yielded negative results in several studies [Curtis and Tilley, 1968;Sawada et al, 1991;Uryvaeva and Delone, 1995]. An induction of micronuclei was reported in two studies in which the frequency of micronuclei was measured during the wave of cell proliferation that followed extensive liver toxicity [Van Goethem et al, 1993.…”

Section: Overview Of the Genotoxicity And Mutagenicity Studies On Ctmentioning

confidence: 96%

Evaluating genotoxicity data to identify a mode of action and its application in estimating cancer risk at low doses: A case study involving carbon tetrachloride

Eastmond

2008

Environ and Mol Mutagen

View full text Add to dashboard Cite

In the new USEPA cancer risk assessment guidelines, mode of action (MoA) information, combined with a determination of whether or not a chemical is mutagenic, plays an important role in determining whether a linear or nonlinear approach should be used to estimate cancer risks at low doses. In this article, carbon tetrachloride (CT) is used as an example to illustrate how mixed genotoxicity data can be evaluated and used to identify a likely MoA. CT is essentially negative in inducing gene mutations in Salmonella, but is consistently positive in inducing recombination and aneuploidy in fungi. Negative or equivocal results were seen in most in vitro and in vivo studies in mammals, including mutation studies in transgenic mice. However, DNA adducts, primarily those derived from oxidation- and lipid-peroxidation-derived products as well as DNA double-strand breaks and micronucleated cells, have been seen repeatedly in the liver of CT-treated animals. On the basis of the weight of evidence, CT should not be considered a directly mutagenic agent. Mutagenic as well as other genotoxic effects, as they occur, will most likely be generated through indirect mechanisms resulting from oxidative and lipid peroxidative damage and/or damage occurring during necrosis or apoptosis. As key events in this process are expected to occur in a nonlinear fashion, the expected relationship between CT dose and carcinogenic response in the liver is likely to be nonlinear with a steep dose response. This conclusion is consistent with rodent cancer bioassay results in which steep nonlinear dose responses have been seen.

show abstract

Section: Overview Of the Genotoxicity And Mutagenicity Studies On Ctmentioning

confidence: 96%

Evaluating genotoxicity data to identify a mode of action and its application in estimating cancer risk at low doses: A case study involving carbon tetrachloride

Eastmond

2008

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Our findings are in good agreement with other investigations conducted on mammals, which noted that the MN frequency correlates with increased variability in chromosome number. Uryvaeva and Delone (1995) showed that liver cells from mouse carrying MN were the higher ploides rather than the population in general and Ciranni et al (1995) also found a significant presence of types of MN that are probably aneuploid related. Elhajouji et al (1998) reported about increasing frequency of MN in tetraploid lymphocytes.…”

Section: Original Papermentioning

confidence: 99%

“…As different investigators relate MN appearance with polyploidy and aneuploidy in mammalian cells caused by different genotoxic compounds or treatment (Ciranni et al, 1995;Uryvaeva and Delone, 1995;Elhajouji et al, 1998;Miller et al, 1998;Gonzalez-Cid et al, 1999), the aim of our study was to establish MN appearance in triploid rainbow trout. To the best of the author's knowledge this is the first study of this kind conducted on triploid fish.…”

mentioning

confidence: 99%

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

Strunjak‐Perović¹,

Čož‐Rakovac²,

Popović³

2003

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of the study was to observe the influence of different ploidy levels in fish on micronucleus occurrence. Twenty minutes a�er fertilization, one group of rainbow trout eggs was exposed to water temperatures of 26°C in duration of 20 minutes to induce triploidy. Second group was kept in water temperature of 10°C, which is optimal for development of rainbow trout. The frequency of micronucleated erythrocytes was determined in the peripheral circulation of rainbow trout 67 days (following absorption of the yolk -swim-up stage) and 128 days (fry stage) post fertilization. There was a significant difference (P < 0.001) between frequency of micronucleated erythrocytes of diploid (1.10 ± 0.96‰) and triploid (2.41 ± 1.28‰) fish at swim-up stage. Increased mean values of micronucleus in diploid (1.80 ± 1.57‰) and triploid (5.92 ± 3.80‰) fry were also recorded.

show abstract

“…All postmitotic micronucleated thyroid cells become polyploid. Presumably, this phenomenon is a specific feature of the studied cells: in the hepatocyte population micronuclei are observed mainly in the tetra-and octoploid cells, though can be sometimes seen in diploid elements [12]. In diploid lymphocyte cultures prestimulated to division, mitosis in aberrant cells is completed, and hence, in order to label the pool of proliferating lymphocytes, they are artificially transformed into polyploid ones by means of cytochalasin B (cytotomy blocker) [9].…”

Section: Resultsmentioning

confidence: 98%

Ploidy of micronucleated thyrocytes induced by methylnitrosourea injection

Павлов

Gansburgskii

2005

Bull Exp Biol Med

View full text Add to dashboard Cite

Ploidy of thyroid parenchyma was studied in adult rats in the control and on day 9 after hemithyroidectomy; operated animals received 3 injections of N-nitroso-N-methylurea. The population of follicular thyrocytes is mainly diploid; total count of polyploid cells increased from 4.4% in the control to 8.5% in experimental rats. All thyrocytes containing micronuclei were tetraploid. No diploid micronucleated elements were detected. This suggests that genetically damaged thyrocytes divide by the mechanism of acytokinetic (polyploidizing) mitosis.

show abstract

An improved method of mouse liver micronucleus analysis: an application to age-related genetic alteration and polyploidy study

Cited by 26 publications

References 28 publications

Evaluating genotoxicity data to identify a mode of action and its application in estimating cancer risk at low doses: A case study involving carbon tetrachloride

Evaluating genotoxicity data to identify a mode of action and its application in estimating cancer risk at low doses: A case study involving carbon tetrachloride

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

Ploidy of micronucleated thyrocytes induced by methylnitrosourea injection

Contact Info

Product

Resources

About