“…Now there are many extraction methods of genomic DNA from biomaterials. The commonly employed DNA isolation methods involve the use of enzymes such as lysozyme and proteinase K (Lockhart et al, 1989), cetytrime-thylammonium bromice (CTAB) (Ghosh et al, 2009;Moyo et al, 2008;Khanuja et al, 1999;Novaes et al, 2009;Singh et al, 2000) or sodium dodecyl sulfate (SDS) (Kaufman et al, 1999;Dellaporta et al, 1983) treatment and extraction with organic solvents, detergent-induced lysis in conjunction with proteinase K and lysozyme (Perera et al, 1994) or lysis using guanidinium isothiocyanate (GITC)-containing solutions (Boom et al, 1990;Noordhoek et al, 1995;Chakravorty & Tyagi, 2001), among which, guanidinium thiocyanate has been shown to be a powerful agent in the purification of DNA because of its potential to lyse cells and its potential to inactivate nuclease (Boom et al, 1990;Chomczynski et al, 1987;Zeillinger et al, 1993). However, high amounts of gummy polysaccharides, polyphenols and other various secondary metabolites such as alkaloids, flavonoids, terpenes and tannins in the desert plants usually hamper the DNA isolation procedures and reactions such as DNA restriction, amplification and cloning (Moyo et al, 2008;Khanuja et al, 1999;Pang et al, 2011;Zhang K., 2011;Ji & Li, 2011).…”