An improved method for transformation of Actinidia arguta utilized to demonstrate a central role for MYB110 in regulating anthocyanin accumulation in kiwiberry

Herath, Dinum; Wang, Tianchi; Peng, Yongyan; Allan, Andrew C.; Putterill, Joanna; Varkonyi‐Gasic, Erika

doi:10.1007/s11240-020-01915-1

Cited by 8 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most kiwifruit species ( Actinidia spp.) have the ability to synthesize anthocyanins; pigmentation is frequently observed in young leaves, flowers and tissues undergoing stress (Montefiori et al ., 2009; Herath et al ., 2020). While most cultivars have no anthocyanic colour in the fruit, anthocyanins can still be found in the petioles and young leaves of these cultivars (Montefiori et al ., 2009).…”

Section: Introductionmentioning

confidence: 99%

The red flesh of kiwifruit is differentially controlled by specific activation–repression systems

et al. 2022

Self Cite

View full text Add to dashboard Cite

Anthocyanins are visual cues for pollination and seed dispersal. Fruit containing anthocyanins also appeals to consumers due to its appearance and health benefits. In kiwifruit (Actinidia spp.) studies have identified at least two MYB activators of anthocyanin, but their functions in fruit and the mechanisms by which they act are not fully understood.Here, transcriptome and small RNA high-throughput sequencing were used to comprehensively identify contributors to anthocyanin accumulation in kiwifruit.Stable overexpression in vines showed that both 35S::MYB10 and MYB110 can upregulate anthocyanin biosynthesis in Actinidia chinensis fruit, and that MYB10 overexpression resulted in anthocyanin accumulation which was limited to the inner pericarp, suggesting that repressive mechanisms underlie anthocyanin biosynthesis in this species. Furthermore, motifs in the C-terminal region of MYB10/110 were shown to be responsible for the strength of activation of the anthocyanic response. Transient assays showed that both MYB10 and MYB110 were not directly cleaved by miRNAs, but that miR828 and its phased small RNA AcTAS4-D4(−) efficiently targeted MYB110. Other miRNAs were identified, which were differentially expressed between the inner and outer pericarp, and cleavage of SPL13, ARF16, SCL6 and F-box1, all of which are repressors of MYB10, was observed.We conclude that it is the differential expression and subsequent repression of MYB activators that is responsible for variation in anthocyanin accumulation in kiwifruit species.

show abstract

Section: Introductionmentioning

confidence: 99%

The red flesh of kiwifruit is differentially controlled by specific activation–repression systems

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…In kiwifruit, AcMYB10 and AcMYB110 from Actinidia chinensis activate the CHS, DFR, flavonoid-3-galattosyltransferase (F3GT) and LDOX genes (Li et al, 2017a;Herath et al, 2020), but the regulation of the branch points F3ʹH and F3ʹ5ʹH, which determine the production of cyanidin-or delphinidin-based anthocyanins, respectively, has been attributed to MYBC1 and WRKY44, which are also activators of the biosynthetic genes leading to proanthocyanidins (i.e., FLS, LAR, and ANR; Peng et al, 2020). In the proposed model, the MBW complex including MYB110 activates CHS, DFR, and F3GT, but when MYBC1 interacts with bHLH, WD40, and WRKY44, an MBWW complex (Figure 2D) activates the F3ʹH and F3ʹ5ʹH genes, allowing both the anthocyanin and proanthocyanidin synthesis (Peng et al, 2020).…”

Section: Activators and Repressors Of Anthocyanin Biosynthesis In Fruits Vegetables And Other Cerealsmentioning

confidence: 99%

Anthocyanins: From Mechanisms of Regulation in Plants to Health Benefits in Foods

et al. 2021

View full text Add to dashboard Cite

Anthocyanins represent the major red, purple, and blue pigments in many flowers, fruits, vegetables, and cereals. They are also recognized as important health-promoting components in the human diet with protective effects against many chronic diseases, including cardiovascular diseases, obesity, and cancer. Anthocyanin biosynthesis has been studied extensively, and both biosynthetic and key regulatory genes have been isolated in many plant species. Here, we will provide an overview of recent progress in understanding the anthocyanin biosynthetic pathway in plants, focusing on the transcription factors controlling activation or repression of anthocyanin accumulation in cereals and fruits of different plant species, with special emphasis on the differences in molecular mechanisms between monocot and dicot plants. Recently, new insight into the transcriptional regulation of the anthocyanin biosynthesis, including positive and negative feedback control as well as epigenetic and post-translational regulation of MYB-bHLH-WD40 complexes, has been gained. We will consider how knowledge of regulatory mechanisms has helped to produce anthocyanin-enriched foods through conventional breeding and metabolic engineering. Additionally, we will briefly discuss the biological activities of anthocyanins as components of the human diet and recent findings demonstrating the important health benefits of anthocyanin-rich foods against chronic diseases.

show abstract

“…In A. kolomikta, shoot elongation was obtained only when an endosperm-derived callus with adventitious shoot primordia were cultured on ZEA (Asakura and Hoshino 2017). The successful use of ZEA for shoot regeneration was reported for Actinidia tissue after transformation or cryopreservation treatments (Wang et al 2006;Mathew et al 2018;Mathew et al 2019;Herath et al 2020;Pathirana et al 2021). A novel derivative of the family of aromatic cytokinins, mT, has revealed promising results in plant organ culture studies (Jameson 2017), also for Actinidia taxon (Saeiahagh et al, 2019;Debenham and Pathirana 2021).…”

Section: Callus Induction and Plant Regenerationmentioning

confidence: 99%

“…Moreover, anthocyanins from the callus extract can show antioxidant and protective actions (Nazir et al 2019). The report of Herath et al (2020) pointed out that the anthocyanin accumulation could be desirable in Actinidia cultivation.…”

Section: Callus Induction and Plant Regenerationmentioning

confidence: 99%

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

Abdullah

Śliwińska

Góralski

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, results of plant regeneration and acclimatization from the endosperm-derived calli of four cultivars of Actinidia arguta has been presented. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli. On AEM, the source of explant did not significantly affect the rate of callus induction for any of the tested cultivars; no organogenic events were observed. In contrast, on CIM both the source of explants and the cultivar origin caused significant differences in callus formation and subsequent organogenic events. Histological and ultrastructural analyses revealed the adventitious nature of shoot bud formation on these media. The most efficient elongation of shoot buds was achieved after transferring organogenic calli with adventitious shoot buds to a medium supplemented with zeatin or meta-topolin. Robust root induction with minimal basal callus formation occurred on the medium with indole-3-acetic acid. Flow cytometric analysis revealed that the nuclear DNA content in the leaves of some regenerants was approximately 50 % higher (4.5 pg/2C) than that in leaves from the tetraploid seedlings (3.1 pg/2C),which confirmed that those regenerants originated from the endosperm. The regeneration of such hexaploid plants was more efficient when endosperm from fresh seeds served as an explant; therefore, fresh rather than dry seeds are recommended for endosperm-derived plant production. The hexaploid plants of A. arguta can serve as an important source of breeding material.

show abstract

An improved method for transformation of Actinidia arguta utilized to demonstrate a central role for MYB110 in regulating anthocyanin accumulation in kiwiberry

Cited by 8 publications

References 26 publications

The red flesh of kiwifruit is differentially controlled by specific activation–repression systems

The red flesh of kiwifruit is differentially controlled by specific activation–repression systems

Anthocyanins: From Mechanisms of Regulation in Plants to Health Benefits in Foods

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

Contact Info

Product

Resources

About