An improved method for the cyclotron production of 13N-labelled ammonia

Vaalburg, W; Kamphuis, Janine; Molen, H.D. Beerling-van der; Reiffers, S.; Rijskamp, A.; Woldring, Mg

doi:10.1016/0020-708x(75)90169-6

Cited by 105 publications

(34 citation statements)

References 2 publications

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“…The chemical purification and conversion procedure was carried out in a lead-shielded fumehood at the UBC Botany Department and was as follows (in modification of H.J. Kronzucker et al: Compartmental analysis of NH~ in spruce Vaalburg et al 1975, Meeks et al 1978, and Wang et al 1993a): Approximately 15 cm 3 of irradiated target solution were taken up by a 20-cm 3 syringe and passed twice through a SEP-PAC Alumina-N cartridge (Waters Associates, Milford, Mass., USA) to remove the radiocontaminant I SF. To remove residual volume, 5 cm 3 of 2.5 gM Ca(NO3) 2 was used to rinse the cartridge.…”

Section: Methodsmentioning

confidence: 99%

Compartmentation and flux characteristics of ammonium in spruce

Kronzucker

Siddiqi

Glass

1995

Planta

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Section: Methodsmentioning

confidence: 99%

Compartmentation and flux characteristics of ammonium in spruce

Kronzucker

Siddiqi

Glass

1995

Planta

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“…Coni aminants in the %o3-sample (mainly "F) were removed by passing the samples twice through a SEP-PAC Alumina-N cartridge (Waters Associates). Reduction of I3NO3-to ':'NH3 was achieved by using Devarda's alloy at 7OoC in a water bath (Vaalburg et al, 1975;Meeks et al, 1978); I3NH3 was separated from remaining species by distillation and tra pped in acid solution as 13NH4+.…”

Section: Production Of 13nh4+mentioning

confidence: 99%

Ammonium Uptake by Rice Roots (I. Fluxes and Subcellular Distribution of 13NH4+)

et al. 1993

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“…The irradiated water contains predominantly 1 3~O ; with some 13NO;, with yields of up to 100 mCi ml-l. I3N0; and 13NO; were converted to 1 3 N~: with de Varda's alloy (Gelbard et al 1975, Vaalberg et al 1975. Irradiated water was transferred from the target by remote application of pressure into a vessel containing 0.5 g of de Varda's alloy and 0.5 g of NaOH.…”

Section: Methodsmentioning

confidence: 99%

“…'3N-ammonium was produced by the 160(p,a)13N reaction (Cooper et al 1985) bombarding 1 m1 of 1 6 0 water with 17 MeV protons using the BNL Division of Chemistry Japan Steel Works 41 inch BC1710 cyclotron (Vaalberg et al 1975). The irradiated water contains predominantly 1 3~O ; with some 13NO;, with yields of up to 100 mCi ml-l. I3N0; and 13NO; were converted to 1 3 N~: with de Varda's alloy (Gelbard et al 1975, Vaalberg et al 1975.…”

Section: Methodsmentioning

confidence: 99%

Use of 13N as tracer for bacterial and algal uptake of ammonium from sea-water

Fuhrman¹,

Horrigan²,

Capone³

1988

Mar. Ecol. Prog. Ser.

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The relative importance of phytoplankton and heterotrophlc bacteria in the ut~lization of ammonium in a temperate coastal pelaglc environment (Long Island Sound. New York, USA) was examined uslng the short-lived radioisotope, I3N Uptake of ' 3~~: Into different slze fractions under simulated in situ temperature and light condihons was compared to size fractionations of bacterial abundance, chlorophyll, and uptake of tritlated thymidme and 14C-b~carbonate In January and Apnl, little ' 3~ entered the bactenal slze fract~on, but when bacteria were more metabolically a c t~v e in May and July, about '/3 of the total I3N uptake appeared in the size fraction conta~ning most of the bactena but httle of the chlorophyll (0 2 to 0 6 ,pm) Exposure to up to 8 mC1 I-' of I3N radioactivity d~d not ~n h~b~t bacterial or phytoplankton activity, and hlled controls showed virtually no uptake Ammonium uptake rates measured by I3N were comparable to those measured by the stable isotope 15N in 2 of 3 experiments The size fract~onation results were consistent wlth estimates of bactenal and phytoplankton demand tor nitrogen for growth Turnover rates for the dissolved a m m o n~u m pool ranged from 0 6 % h-' in April to 116 O/ O h-' in July In separate experiments .r\~th mlxed seawater cultures of bactena, the half-saturation constant (K,) for ammonium uptake was 1 0 1 uM, and ammonium uptake was reduced more by additions (100 to 1000 nM) of glutamine than by glutamate or othel ainlno a c~d s suggesting that glutamlne is directly ~nvolved in a m m o n~u m uptake In these bacteria

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An improved method for the cyclotron production of 13N-labelled ammonia

Cited by 105 publications

References 2 publications

Compartmentation and flux characteristics of ammonium in spruce

Compartmentation and flux characteristics of ammonium in spruce

Ammonium Uptake by Rice Roots (I. Fluxes and Subcellular Distribution of 13NH4+)

Use of 13N as tracer for bacterial and algal uptake of ammonium from sea-water

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