An Improved Method for the Preparation of Type I Collagen From Skin

Pacak, Christina A.; MacKay, Allison A.; Cowan, Douglas B.

doi:10.3791/51011

Cited by 12 publications

(9 citation statements)

References 13 publications

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“…Collagen can be collected from various biological sources including bovine skin, rat tail and human placenta (Elsdale and Bard, 1972; Pacak et al, 2014). Collagen-based hydrogels have been widely used for the construction of 3D tumor models (Chen et al, 2012; Ingber, 2008; Szot et al, 2011; Yip and Cho, 2013).…”

Section: Matrix-assisted Assembly Of 3d Tumor Modelsmentioning

confidence: 99%

Three-dimensional in vitro tumor models for cancer research and drug evaluation

Farach-Carson

Jia

2014

Biotechnology Advances

398

294

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Cancer occurs when cells acquire genomic instability and inflammation, produce abnormal levels of epigenetic factors/proteins and tumor suppressors, reprogram the energy metabolism and evade immune destruction, leading to the disruption of cell cycle/normal growth. An early event in carcinogenesis is loss of polarity and detachment from the natural basement membrane, allowing cells to form distinct three-dimensional (3D) structures that interact with each other and with the surrounding microenvironment. Although valuable information has been accumulated from traditional in vitro studies in which cells are grown on flat and hard plastic surfaces (2D culture), this culture condition does not reflect the essential features of tumor tissues. Further, fundamental understanding of cancer metastasis cannot be obtained readily from 2D studies because they lack the complex and dynamic cell-cell communications and cell-matrix interactions that occur during cancer metastasis. These shortcomings, along with lack of spatial depth and cell connectivity, limit the applicability of 2D cultures to accurate testing of pharmacologically active compounds, free or sequestered in nanoparticles. To recapitulate features of native tumor microenvironments, various biomimetic 3D tumor models have been developed to incorporate cancer and stromal cells, relevant matrix components, and biochemical and biophysical cues, into one spatially and temporally integrated system. In this article, we review recent advances in creating 3D tumor models employing tissue engineering principles. We then evaluate the utilities of these novel models for the testing of anticancer drugs and their delivery systems. We highlight the profound differences in responses from 3D in vitro tumors and conventional monolayer cultures. Overall, strategic integration of biological principles and engineering approaches will both improve understanding of tumor progression and invasion and support discovery of more personalized first line treatments for cancer patients.

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Section: Matrix-assisted Assembly Of 3d Tumor Modelsmentioning

confidence: 99%

Three-dimensional in vitro tumor models for cancer research and drug evaluation

Farach-Carson

Jia

2014

Biotechnology Advances

398

294

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“…Collagen can also be extracted in large quantities, cheaply and in relatively high purity from a wide range of tissue sources (including skin, tendon, etc.) using a simple acid extraction procedure followed by neutralization (73)(74)(75)(76).…”

Section: Advantages Of Collagen For Myocardial Tementioning

confidence: 99%

Natural Biomaterials for Cardiac Tissue Engineering: A Highly Biocompatible Solution

Majid

Fricker

Gregory

et al. 2020

Front. Cardiovasc. Med.

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Cardiovascular diseases (CVD) constitute a major fraction of the current major global diseases and lead to about 30% of the deaths, i.e., 17.9 million deaths per year. CVD include coronary artery disease (CAD), myocardial infarction (MI), arrhythmias, heart failure, heart valve diseases, congenital heart disease, and cardiomyopathy. Cardiac Tissue Engineering (CTE) aims to address these conditions, the overall goal being the efficient regeneration of diseased cardiac tissue using an ideal combination of biomaterials and cells. Various cells have thus far been utilized in pre-clinical studies for CTE. These include adult stem cell populations (mesenchymal stem cells) and pluripotent stem cells (including autologous human induced pluripotent stem cells or allogenic human embryonic stem cells) with the latter undergoing differentiation to form functional cardiac cells. The ideal biomaterial for cardiac tissue engineering needs to have suitable material properties with the ability to support efficient attachment, growth, and differentiation of the cardiac cells, leading to the formation of functional cardiac tissue. In this review, we have focused on the use of biomaterials of natural origin for CTE. Natural biomaterials are generally known to be highly biocompatible and in addition are sustainable in nature. We have focused on those that have been widely explored in CTE and describe the original work and the current state of art. These include fibrinogen (in the context of Engineered Heart Tissue, EHT), collagen, alginate, silk, and Polyhydroxyalkanoates (PHAs). Amongst these, fibrinogen, collagen, alginate, and silk are isolated from natural sources whereas PHAs are produced via bacterial fermentation. Overall, these biomaterials have proven to be highly promising, displaying robust biocompatibility and, when combined with cells, an ability to enhance post-MI cardiac function in pre-clinical models. As such, CTE has great potential for future clinical solutions and hence can lead to a considerable reduction in mortality rates due to CVD.

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“…Collagen is extracted from skin tissue by first removing fat deposits followed by stripping non-collagenous proteins and polysaccharides with sodium acetate, followed by extraction of collagen from the tissue with sodium citrate [29]. Sodium citrate as a mild base serves as a solubilizing agent for collagen molecules, thus the triple-helical and fibrillar structure of collagen remains relatively intact after isolation.…”

Section: Structure Of Protein-based Hydrogelsmentioning

confidence: 99%

Challenges for Natural Hydrogels in Tissue Engineering

Jabbari

2019

Gels

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Protein-based biopolymers derived from natural tissues possess a hierarchical structure in their native state. Strongly solvating, reducing and stabilizing agents, as well as heat, pressure, and enzymes are used to isolate protein-based biopolymers from their natural tissue, solubilize them in aqueous solution and convert them into injectable or preformed hydrogels for applications in tissue engineering and regenerative medicine. This review aims to highlight the need to investigate the nano-/micro-structure of hydrogels derived from the extracellular matrix proteins of natural tissues. Future work should focus on identifying the nature of secondary, tertiary, and higher order structure formation in protein-based hydrogels derived from natural tissues, quantifying their composition, and characterizing their binding pockets with cell surface receptors. These advances promise to lead to wide-spread use of protein-based hydrogels derived from natural tissues as injectable or preformed matrices for cell delivery in tissue engineering and regenerative medicine.

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An Improved Method for the Preparation of Type I Collagen From Skin

Cited by 12 publications

References 13 publications

Three-dimensional in vitro tumor models for cancer research and drug evaluation

Three-dimensional in vitro tumor models for cancer research and drug evaluation

Natural Biomaterials for Cardiac Tissue Engineering: A Highly Biocompatible Solution

Challenges for Natural Hydrogels in Tissue Engineering

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