AN IMPROVED METHOD FOR THE DETERMINATION OF TRYPTOPHANE WITH p-DIMETHYLAMINOBENZALDEHYDE

Graham, Claire E.; Smith, Edward P.; Hier, Stanley W.; Klein, David J.

doi:10.1016/s0021-9258(17)30929-8

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Hydrolysis Procedures J1

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1949

1980

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Cited by 73 publications

(9 citation statements)

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“…Cystine and cysteine were converted to cysteic acid (Schram et al, 1954) which was determined with the amino acid analyzer. Tryptophan was determined by the colorimetric method of Graham et al (1947) based on the reaction of p-(dimethylamino)benzaldehyde with tryptophan in the presence of sodium nitrate and hydrochloric acid.…”

Section: Methodsmentioning

confidence: 99%

Amino acid content of Baltic herring and rainbow trout roe

Kaitaranta¹,

Lamppu²,

Linko³

1980

J. Agric. Food Chem.

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Section: Methodsmentioning

confidence: 99%

Amino acid content of Baltic herring and rainbow trout roe

Kaitaranta¹,

Lamppu²,

Linko³

1980

J. Agric. Food Chem.

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“…The increase in indicated tryptophan content and return to normal color of the tests occurred after the hydrolyzates had stood at 5°for one day (Table I). This behavior appears to be related to the reversal of a reaction involving cystine which produces an interfering substance during the hydrolysis (9). However, the reliability of the chemical values determined on hydrolyzates that had stood one day at 5°C.…”

Section: Hydrolysis Procedures Jmentioning

confidence: 99%

Chemical Determination of Tryptophan in Proteins

Spies¹,

Chambers²

1949

Anal. Chem.

1,014

226

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agent in small portions, tapping the absorber gently after each addition, as suggested by Clark and Stillson (4). Care in selecting, handling, and storing Ascarite is also essential, as some lots of this drying agent were not effective.Time Required for Analysis. Table III gives the average time required for the various operations necessary for this procedure. Six to eight determinations can be made in an 8-hour day. The working time can be lessened somewhat by using weighed absorbers from one determination for a subsequent determination and using a smaller sample [50 to 70 mg. have been suggested by Natelson and co-workers (9, 10)].

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“…Norleucin served as an internal standard. Tryptophan was determined by a colorimetric method of Graham et al (1947) and cystine and cysteine together as cysteic acid by a method of Schram et al (1954). Available lysine was assayed by a method of Carpenter (1960) based on a dinitrophenyl derivative of lysine.…”

Section: Methodsmentioning

confidence: 99%