An improved method for rapid analysis of the fatty acids of glycerolipids

Ichihara, Kazuo; Shibahara, Akira; Yamamoto, Kazuhito; Nakayama, Takao

doi:10.1007/bf02522986

Cited by 106 publications

(118 citation statements)

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“…N 40°51′37,59″ Lon. E 15°38′49,43″) on a Luvi-vertic Phaeozem [29], loam soil, in the period JuneDecember 2013. A field randomized block design with three replications was established to test two strategies of Nitrogen fertilization:…”

Section: Plants and Growth Conditionsmentioning

confidence: 99%

Nutritional quality of seeds and leaf metabolites of Chia (Salvia hispanica L.) from Southern Italy

Amato

Caruso

Guzzo

et al. 2015

Eur Food Res Technol

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Chia (Salvia hispanica L.) is an expanding functional food crop from Central America, but little is known about effects of new growing environments and agronomical treatments on seed quality. The quality of seeds produced in Europe has never been reported and there’s a lack of information on secondary metabolites of leaves, an emerging product of Chia. In this work, the nutritional quality and oxidative stability of Chia seeds produced in Southern Italy (Basilicata region) have been evaluated, taking into account also different nitrogen fertilization strategies. The quality of seeds produced in Basilicata has been also compared with that of commercially available seeds from traditional and new production areas. Oil extracted from Italian Chia seeds was not quantitatively significantly different, but more rich in chlorophyll, carotenoids and α-linolenic acid than in commercial seeds. Oxidative stability, polyphenols and antioxidant activity were also influenced by seed source. Fertilization system affected free acidity, chlorophyll and carotenoids, whereas it reduced p-Anisidine value, phenols and oxidative stability. Moreover, the metabolic profile of Chia leaves has been reported for the first time; in particular, several flavonoids and hydroxycinnamic acids, and the two uncommon flavonoids acetyl vitexin and acetyl orientin were found

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Section: Plants and Growth Conditionsmentioning

confidence: 99%

Nutritional quality of seeds and leaf metabolites of Chia (Salvia hispanica L.) from Southern Italy

Amato

Caruso

Guzzo

et al. 2015

Eur Food Res Technol

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“…The fatty acid (FA) composition of EM ghost was determined by gas liquid chromatography (GLC, Agilent Technologies India Pvt. Ltd., Mumbai) techniques after converting the lipid into their corresponding fatty acid methyl esters (FAME) according to the method as previously described [21].…”

Section: Analysis Of Fatty Acid Composition Of Em Ghostmentioning

confidence: 99%

“…Ltd., Mumbai) techniques after converting the oils (triglycerides) into their corresponding fatty acid methyl esters (FAME) as per Ichihara et al [21]. The amount of cis-trans isomers present in the oil was determined by dissolving the oil in cyclohexane and measuring the absorbance over wavelength range of 200-300 nm by a UV-Vis Spectrophotometer (Shimadzu corporation, Japan) [22].…”

Section: Analysis Of Fatty Acid Compositionsmentioning

confidence: 99%

Comparative study of hypocholesterolemic and hypolipidemic effects of conjugated linolenic acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity

Saha¹,

Chakraborty

Ghosh³

et al. 2011

Eur J Nutr

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“…The fatty acid methyl esters of experimental diets and whole body fish samples were prepared using the method described by Ichiara et al (1996) with a minor modification-through transmethylation using 2 M KOH in methanol and n-heptane. The fatty acids were analysed by a GC Clarus 500 with autosampler (Perkin Elmer, USA) equipped with a flame ionization detector and a fused silica capillary SGE column (30 m × 0.32 mm, ID × 0.25 µm, BP20 0.25 UM, USA).…”

Section: Sample Collection and Analytical Methodsmentioning

confidence: 99%