An Improved Method for Rapid Intubation of the Trachea in Mice

Vandivort, Tyler C.; An, Dowon; Parks, William C.

doi:10.3791/53771

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…Therefore, the drug solution was delivered to the lung more efficiently and with greater certainty than when using positive pressure. Recently, many researchers have reported methods or procedures of non-invasive intratracheal intubation [19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice

Oiso¹,

Akita

Kato³

et al. 2020

Pharmaceutics

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When developing inhaled medicines for respiratory diseases, such as chronic obstructive pulmonary disease, drugs need to be administered by pulmonary delivery to animals in non-clinical tests. Common methods require application of pressure during administration, and it may cause lung injury, so we focused on the inhalation of liquid medicines by mice themselves. This study aimed to evaluate a negative pressure method of pulmonary administration in mice by self-inhalation. First, to confirm the accuracy of delivery of liquid medicines into lungs and the potential for lung injury, Institute of Cancer Research (ICR) mice received methylene blue tetrahydrate or saline by the negative pressure method. We assessed drug distribution and usefulness of this method by administering porcine pancreatic elastase and all-trans-retinoic acid (ATRA) to mice. Consequently, we confirmed good distribution of the dye and no injury such as disruption of blood flow or destruction of alveoli in lungs of mice. Following production of the murine emphysema model, the mean linear intercept (Lm) was calculated as 78 ± 4 μm. Moreover, a significant therapeutic effect of administration of the ATRA was confirmed. These results suggest that this negative pressure method of administration may be useful for pulmonary administration in non-clinical tests.

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Section: Discussionmentioning

confidence: 99%

Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice

Oiso¹,

Akita

Kato³

et al. 2020

Pharmaceutics

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“…After confirming sedation by monitoring the sedation parameters, the chest of mice was cleaned aseptically with povidone-iodine solution and shaved. The anesthetized mice were positioned into the intubation platform having 60-80 degrees to visualize the epiglottis [9] . After stabilizing the mouth of the mouse, the tongue was lifted by the help of forceps, and a fiber optic cable connected to the light source was placed to the throat to visualize the movement of the epiglottis.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

et al. 2021

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Retrograde dyes are often used in basic research to investigate neuronal innervations of an organ. This article describes the experimental data on the application of retrograde dyes on the mouse heart in vivo and on the cardiac or neuronal cultures in vitro. By providing this information, cardiac or inneinnervations can be evaluated in vivo . Therefore, unknown cellular and molecular mechanisms and systemic interactions in the body can be investigated. In particular, we provided practical tips to lower mortality risks following the cardiac surgery and evaluated the staining capacity and fluorescent characteristics of the Di-8-ANEPPQ dye in the cardiac tissue and cell cultures. First, primary cultures of mouse nodose ganglia (NG) neurons and mouse neonatal cardiomyocytes were stained with Di-8-ANEPPQ. The Di-8-ANEPPQ signal from live cultures were visualized using spinning disk confocal microscopy to verify the lipophilic and fluorescent labeling capacity of Di-8-ANEPPQ. Next, the excitation and emission data of Di-8-ANEPPQ were collected between 415 nm and 690 nm using power spectrum module of confocal microscopy. This spectrum analysis could be useful for the researchers who plan to use Di-8-ANEPPQ in combination with other fluorescent dyes to eliminate any florescent overlap. In order to label the heart tissue with tracer dyes Di-8-ANEPPQ or DiI in vivo , the heart was exposed without damaging lungs or other tissues following anesthetization, then the retrograde dye was applied as a paste for DiI or injected to the apex of the heart for Di-8-ANEPPQ and the operation area was sutured. The surgical procedure required intubation to control the respiratory reflex without the need to perform a tracheotomy and yielded high viability. Following labeling the heart in vivo , the heart was dissected, and images of injection area were captured using confocal microscopy. All fluorescent images of Di-8-ANEPPQ labeled cells were analyzed by using the Fiji software. Overall, these data provide applicable data to other investigators to trace the sensory neurons innervating not only the heart but also other organs using Di-8-ANEPPQ. These data support the original research article titled “Evaluation of bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling” that was accepted for publication in Brain Research Journal [1] .

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“…After bleomycin administration, body weights were monitored every third day. According to the previous report, weight loss is an indicator of successful model construction (Vandivort et al, 2016). Mice with a weight loss of less than 5% at day 7 or less than 10% at day 10 post the bleomycin challenge were excluded from further study.…”

Section: Bleomycin-induced Pulmonary Fibrosis In Micementioning

confidence: 99%

Hyperbaric Oxygen Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice

Yuan

Qiao

et al. 2021

Front. Mol. Biosci.

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The prevalence of pulmonary fibrosis is increasing with an aging population and its burden is likely to increase following COVID-19, with large financial and medical implications. As approved therapies in pulmonary fibrosis only slow disease progression, there is a significant unmet medical need. Hyperbaric oxygen (HBO) is the inhaling of pure oxygen, under the pressure of greater than one atmosphere absolute, and it has been reported to improve pulmonary function in patients with pulmonary fibrosis. Our recent study suggested that repetitive HBO exposure may affect biological processes in mice lungs such as response to wounding and extracellular matrix. To extend these findings, a bleomycin-induced pulmonary fibrosis mouse model was used to evaluate the effect of repetitive HBO exposure on pulmonary fibrosis. Building on our previous findings, we provide evidence that HBO exposure attenuates bleomycin-induced pulmonary fibrosis in mice. In vitro, HBO exposure could reverse, at least partially, transforming growth factor (TGF)-β–induced fibroblast activation, and this effect may be mediated by downregulating TGF-β–induced expression of hypoxia inducible factor (HIF)-1α. These findings support HBO as a potentially life-changing therapy for patients with pulmonary fibrosis, although further research is needed to fully evaluate this.

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An Improved Method for Rapid Intubation of the Trachea in Mice

Cited by 15 publications

References 24 publications

Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice

Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice

Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

Hyperbaric Oxygen Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice

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