An Improved Method for Purification of Sweet Potato Feathery Mottle Virus Directly from Sweet Potato

Cohen, J.; Salomon, R.; Loebenstein, G.

doi:10.1094/phyto-78-809

Cited by 19 publications

(16 citation statements)

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“…The proportion of shorter polypeptides varied with time in partially purified preparations. However, following CsC1 density gradient separation, the virus preparation was stable for at least a year (Cohen et al, 1988). These observations indicated a close association between the virus and a protease that cleaves segments from the coat protein, leaving its core intact.…”

Section: Amino Acid Sequencingmentioning

confidence: 94%

“…When recovered 1. nil plants were pruned the new growth showed symptoms that then disappeared in the upper leaves much as after the initial inoculation. DISCUSSION SPFMV preparations contain four polypeptide products of the coat protein subunit of which three are smaller in size than the full-length coat protein (Moyer & Cali, 1985;Cohen et al, 1988). The proportion of shorter polypeptides varied with time in partially purified preparations.…”

Section: Amino Acid Sequencingmentioning

confidence: 96%

“…Cotyledons of L nil seedlings were mechanically inoculated with extracts from S PFMV-infected L batatas, or 0.05 mg/ml of SPFMV purified from infected L batatas (Cohen et al, 1988).…”

Section: Plant Materialmentioning

confidence: 99%

“…SPFMV was purified from infected L batatas leaves as described by Cohen et al (1988) except that when analysing the effect of protease inhibitors on the viral coat cleavage, a partial purification procedure utilizing polyethylene glycol was used (Alper et al, 1984).…”

Section: Plant Materialmentioning

confidence: 99%

“…Preparations of the coat proteins of potyviruses are often heterogeneous in size and in the relative concentrations of the various polypeptides (Moghal & Francki, 1976;Hiebert et al, 1984;Moyer & Call, 1985;Stein et al, 1986;Cohen et al, 1988) and sweet potato feathery mottle potyvirus (SPFMV) is typical in that coat protein from purified virus particles comprises an Mr 38 000 (38K) polypeptide and several minor smaller polypeptides (Moyer & Call, 1985;Cohen et al, 1988). Although SPFMV-infected sweet potato has some symptomless leaves, Ipomoea nil exhibits more regular symptomless recovery and was therefore selected for this study.…”

Section: Introductionmentioning

confidence: 99%

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Partial Cleavage of Sweet Potato Feathery Mottle Virus Coat Protein Subunit by an Enzyme in Extracts of Infected Symptomless Leaves

Salomon

1989

Journal of General Virology

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SUMMARYThe coat protein of particles of sweet potato feathery mottle potyvirus (SPFMV) extracted from Ipomoea spp. migrated in SDS-PAGE mainly as bands of Mr 38000 (38K), 36K, 32K and 30K. Trypsin treatment of the particles resulted in the appearance of only one 30K polypeptide. The inclusion of protease inhibitors in the extraction procedure did not alter the heterogeneity of SPFMV coat protein. A partially purified fraction of extracts from recovering, symptomless, but not from healthy leaves of L nil had a proteolytic activity similar to that of trypsin. Amino acid sequencing showed that the trypsin-cleaved 30K polypeptide had some sequence homology with other potyvirus coat proteins. The site at which the lpomea extract cleaved the protein was five amino acids nearer the N terminus than the trypsin cleavage site.

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Section: Amino Acid Sequencingmentioning

confidence: 94%

Section: Amino Acid Sequencingmentioning

confidence: 96%

“…Cotyledons of L nil seedlings were mechanically inoculated with extracts from S PFMV-infected L batatas, or 0.05 mg/ml of SPFMV purified from infected L batatas (Cohen et al, 1988).…”

Section: Plant Materialmentioning

confidence: 99%

Section: Plant Materialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Partial Cleavage of Sweet Potato Feathery Mottle Virus Coat Protein Subunit by an Enzyme in Extracts of Infected Symptomless Leaves

Salomon

1989

Journal of General Virology

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Synergistic Interactions of a Potyvirus and a Phloem-Limited Crinivirus in Sweet Potato Plants

Karyeija¹,

Kreuze²,

et al. 2000

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When infecting alone, Sweet potato feathery mottle virus (SPFMV, genus Potyvirus) and Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus) cause no or only mild symptoms (slight stunting and purpling), respectively, in the sweet potato (Ipomoea batatas L. ). In the SPFMV-resistant cv. Tanzania, SPFMV is also present at extremely low titers, though plants are systemically infected. However, infection with both viruses results in the development of sweet potato virus disease (SPVD) characterized by severe symptoms in leaves and stunting of the plants. Data from this study showed that SPCSV remains confined to phloem and at a similar or slightly lower titer in the SPVD-affected plants, whereas the amounts of SPFMV RNA and CP antigen increase 600-fold. SPFMV was not confined to phloem, and the movement from the inoculated leaf to the upper leaves occurred at a similar rate, regardless of whether or not the plants were infected with SPCSV. Hence, resistance to SPFMV in cv. Tanzania was not based on restricted virus movement, neither did SPCSV significantly enhance the phloem loading or unloading of SPFMV. It is also noteworthy that SPVD is an unusual synergistic interaction in that the potyvirus component is not the cause of synergism but is the beneficiary. It is hypothesized that SPCSV is able to enhance the multiplication of SPFMV in tissues other than where it occurs itself, perhaps by interfering with systemic phloem-dependent signaling required in a resistance mechanism directed against SPFMV.

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Virus and Phytoplasma Diseases

Loebenstein

Thottappilly²,

Fuentes

et al. 2009

The Sweetpotato

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An Improved Method for Purification of Sweet Potato Feathery Mottle Virus Directly from Sweet Potato

Cited by 19 publications

References 0 publications

Partial Cleavage of Sweet Potato Feathery Mottle Virus Coat Protein Subunit by an Enzyme in Extracts of Infected Symptomless Leaves

Partial Cleavage of Sweet Potato Feathery Mottle Virus Coat Protein Subunit by an Enzyme in Extracts of Infected Symptomless Leaves

Synergistic Interactions of a Potyvirus and a Phloem-Limited Crinivirus in Sweet Potato Plants

Virus and Phytoplasma Diseases

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